1.1.1.27: L-lactate dehydrogenase

This is an abbreviated version, for detailed information about L-lactate dehydrogenase, go to the full flat file.

Reaction

(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

A4-LDH, AdhE, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, heart LDH, Immunogenic protein p36, L(+)-nLDH, L-(+)-lactate dehydrogenase, L-lactate dehydrogenase B, L-lactic acid dehydrogenase, L-lactic dehydrogenase, L-LDH, lactate dehydrogenase, lactate dehydrogenase A, lactate dehydrogenase B, lactate dehydrogenase NAD-dependent, lactic acid dehydrogenase, lactic dehydrogenase, LctD, LDH, LDH-1, LDH-2, LDH-3, LDH-4, LDH-5, LDH-A, LDH-A4, LDH-m4, LDH1, LDH2, LdhA, LDHB, mLDH, More, muscle LDH, NAD-lactate dehydrogenase, nitric oxideinducible l-lactate dehydrogenase, PfLDH, proteins, specific or class, anoxic stress response, p34, Sa-LDH-1, TeLdhL, Tsac_0416

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.27 L-lactate dehydrogenase

Substrates Products

Substrates Products on EC 1.1.1.27 - L-lactate dehydrogenase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-lactate + 3-acetyl pyridine adenine dinucleotide
pyruvate + reduced 3-acetyl pyridine adenine dinucleotide
show the reaction diagram
-
assay is based on reduction of 3-acetyl pyridine adenine dinucleotide that is specific for PfLDH, which allows the distinction of PfLDH from that of the host erythrocyte
-
-
?
(S)-lactate + 3-acetylpyridine adenine dinucleotide
pyruvate + reduced 3-acetylpyridine adenine dinucleotide
show the reaction diagram
(S)-lactate + NAD(P)+
pyruvate + NAD(P)H + H+
show the reaction diagram
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
(S)-lactate + NADP+
pyruvate + NADPH + H+
show the reaction diagram
wild-type enzyme is specific for NAD+. Mutant enzyme F16Q/I37K/D38SC81S/N85R utilizes NADP+ better than wild-type enzyme, prefers NADP+ to NAD+. Mutant F16Q/C81S/N85R utilizes NAD+ better than wild-type enzyme, weakly active with NADP+
-
-
?
2-oxobutyrate + NADH
2-hydroxybutyrate + NAD+
show the reaction diagram
2-oxoglutarate + NADH + H+
2-hydroxyglutarate + NAD+
show the reaction diagram
2-oxopentanoate + NADH
2-hydroxypentanoate + NAD+
show the reaction diagram
-
125% of the activity with pyruvate
-
-
?
2-oxovalerate + NADH
2-hydroxyvalerate + NAD+
show the reaction diagram
-
-
-
-
r
3-fluoropyruvate + NADH
?
show the reaction diagram
3-methyl-2-oxobutanoate + NADH
2-hydroxy-3-methylbutanoate + NAD+
show the reaction diagram
-
28.5% of the activity with pyruvate
-
-
?
3-methyl-2-oxopentanoate + NADH
2-hydroxy-3-methylpentanoate + NAD+
show the reaction diagram
-
5.3% of the activity with pyruvate
-
-
?
4-methyl-2-oxopentanoate + NADH
2-hydroxy-4-methylpentanoate + NAD+
show the reaction diagram
bromopyruvate + NADH
5-bromo-2-hydroxypropanoate + NAD+
show the reaction diagram
-
-
-
-
?
glyoxylate + NADH
glycolate + NAD+
show the reaction diagram
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
oxamate + NADH
?
show the reaction diagram
-
two distinct active site LDH/NADH-oxamate complex conformations, a major populated structure wherein all significant hydrogen-bonding patterns are formed at the active site between protein and bound ligand necessary for the catalytically productive Michaelis complex and, a minor structure in a configuration of the active site that is unfavorable to carry out catalyzed chemistry. This latter structure likely simulates a dead-end complex in the reaction mixture. The evolution of the encounter complex between LDH/NADH and oxamate collapses via a branched reaction pathway to form the major and minor bound species. Once the encounter complex is formed between LDH/NADH and substrate, the ternary protein-ligand complex appears to fold to form a compact productive complex in an all or nothing like fashion with all the important molecular interactions coming together at the same time
-
-
?
phenylpyruvate + NADH
2-hydroxy-3-phenylpropanoate + NAD+
show the reaction diagram
phenylpyruvate + NADH
phenyllactate + NAD+
show the reaction diagram
pyruvate + NADH
?
show the reaction diagram
-
-
-
-
?
pyruvate + NADH
L-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
lactate + NAD+
show the reaction diagram
pyruvate + NADPH + H+
(S)-lactate + NADP+
show the reaction diagram
pyruvate ethyl ester + NADH
2-hydroxypropanoate ethyl ester
show the reaction diagram
-
-
-
-
?
pyruvate methyl ester + NADH
2-hydroxypropanoate methyl ester
show the reaction diagram
-
-
-
-
?
additional information
?
-