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Literature summary for 1.1.1.27 extracted from

  • Goetz, F.; Schleifer, K.H.
    Purification and properties of a fructose-1,6-diphosphate activated L-lactate dehydrogenase from Staphylococcus epidermidis (1975), Arch. Microbiol., 105, 303-312.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Staphylococcus epidermidis
fructose 1,6-diphosphate concentration required for 50% maximal activity is about 0.15 mM Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive with NADH Staphylococcus epidermidis
AMP
-
Staphylococcus epidermidis
ATP
-
Staphylococcus epidermidis
GTP
-
Staphylococcus epidermidis
oxamate
-
Staphylococcus epidermidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Staphylococcus epidermidis
130000
-
sucrose density gradient centrifugation Staphylococcus epidermidis

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
ATCC 14990
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2220
-
-
Staphylococcus epidermidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+ poor reaction Staphylococcus epidermidis pyruvate + NADH + H+
-
r
pyruvate + NADH + H+
-
Staphylococcus epidermidis (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE Staphylococcus epidermidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
39
-
-
Staphylococcus epidermidis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
2 min, 6% loss of activity in presence of fructose 1,6-diphosphate, NADH and pyruvate, complete loss of activity without protectant Staphylococcus epidermidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
reduction of pyruvate Staphylococcus epidermidis

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.2 pH 5.2: about 35% of maximal activity, pH 7.2: about 50% of maximal activity Staphylococcus epidermidis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
highest stability at Staphylococcus epidermidis

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Staphylococcus epidermidis
NADH coenzyme Staphylococcus epidermidis