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Literature summary for 1.1.1.27 extracted from

  • Skory, C.; Mertens, J.; Rich, J.
    Inhibition of Rhizopus lactate dehydrogenase by fructose 1,6-bisphosphate (2009), Enzyme Microb. Technol., 44, 242-247.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information presence of an amino terminal fusion with a small ubiquitin-related modifier, SUMO, increases the oxidative activity per micromol protein by more than 100fold, while having little effect on the reductive LDH activity Rhizopus delemar
additional information presence of an amino terminal fusion with a small ubiquitin-related modifier, SUMO, increases the oxidative activity per micromol protein by more than 100fold, while having little effect on the reductive LDH activity Rhizopus oryzae ATCC 9363

Cloned(Commentary)

Cloned (Comment) Organism
into pET-SUMO and expressed as 6× His Tag in Escherichia coli BL21 Rhizopus delemar
into pET-SUMO and expressed as 6× His Tag in Escherichia coli BL21 Rhizopus oryzae ATCC 9363

Inhibitors

Inhibitors Comment Organism Structure
fructose 1,6-bisphosphate 10 mM significantly inhibits LDHB by 23% in a non-competitive manner. Level of inhibition seems to be even more pronounced at pH 6.2, compared to the optimal pH 6.8. At the more acidic pH and in the presence of 10 mM, LDHB shows a 42% decrease in activity Rhizopus delemar
fructose 1,6-bisphosphate 10 mM significantly inhibits LDHA abd LDHB (by 86%) in a non-competitive manner. Level of inhibition seems to be even more pronounced at pH 6.2, compared to the optimal pH 6.8. At the more acidic pH and in the presence of 10 mM, there is almost a 97% decrease in activity for LDHB Rhizopus oryzae ATCC 9363

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
pyruvate LDHB, in the presence of 0.25 mM NADH Rhizopus oryzae ATCC 9363
1.9
-
pyruvate LDHB, in the presence of 0.2 mM NADH Rhizopus oryzae ATCC 9363
2.8
-
pyruvate LDHB, in the presence of 0.15 mM NADH Rhizopus oryzae ATCC 9363
4.8
-
pyruvate LDHB, in the presence of 0.125 mM NADH Rhizopus oryzae ATCC 9363
5
-
pyruvate LDHB, in the presence of 0.25 mM NADH Rhizopus delemar
7.7
-
pyruvate LDHB, in the presence of 0.2 mM NADH Rhizopus delemar
8.4
-
pyruvate LDHB, in the presence of 0.15 mM NADH Rhizopus delemar
15
-
pyruvate LDHB, in the presence of 0.125 mM NADH Rhizopus delemar
47.4
-
Lactate LDHB, in the presence of 3.75 mM NAD+ Rhizopus oryzae ATCC 9363
105.1
-
Lactate LDHB, in the presence of 3.75 mM NAD+ Rhizopus delemar

Organism

Organism UniProt Comment Textmining
Rhizopus delemar
-
-
-
Rhizopus delemar RA 99-880
-
-
-
Rhizopus oryzae ATCC 9363
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on cobalt affinity resin Rhizopus delemar
on cobalt affinity resin Rhizopus oryzae ATCC 9363

Storage Stability

Storage Stability Organism
4°C, glycerol and DDT containing buffer Rhizopus delemar
4°C, glycerol and DDT containing buffer Rhizopus oryzae ATCC 9363

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+
-
Rhizopus delemar lactate + NAD+
-
?
pyruvate + NADH + H+
-
Rhizopus oryzae ATCC 9363 lactate + NAD+
-
?
pyruvate + NADH + H+
-
Rhizopus delemar RA 99-880 lactate + NAD+
-
?

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
Rhizopus delemar
lactate dehydrogenase
-
Rhizopus oryzae ATCC 9363
LdhA
-
Rhizopus oryzae ATCC 9363
LDHB
-
Rhizopus delemar
LDHB
-
Rhizopus oryzae ATCC 9363

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
142
-
pyruvate LDHB, in the presence of 0.125 mM NADH Rhizopus delemar
154
-
pyruvate LDHB, in the presence of 0.15 mM NADH Rhizopus delemar
197
-
pyruvate LDHB, in the presence of 0.125 mM NADH Rhizopus oryzae ATCC 9363
244
-
pyruvate LDHB, in the presence of 0.15 mM NADH Rhizopus oryzae ATCC 9363
298
-
pyruvate LDHB, in the presence of 0.2 mM NADH Rhizopus delemar
318
-
pyruvate LDHB, in the presence of 0.2 mM NADH Rhizopus oryzae ATCC 9363
320
-
pyruvate RLDHB, in the presence of 0.25 mM NADH Rhizopus oryzae ATCC 9363
350
-
pyruvate LDHB, in the presence of 0.25 mM NADH Rhizopus delemar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Rhizopus delemar
6.8
-
-
Rhizopus oryzae ATCC 9363

pH Range

pH Minimum pH Maximum Comment Organism
6.4 6.9 LDHB, in Bis-Tris-propane buffer Rhizopus delemar
6.4 6.9 LDHB, in Bis-Tris-propane buffer Rhizopus oryzae ATCC 9363

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2
-
fructose 1,6-bisphosphate LDHA Rhizopus oryzae ATCC 9363
3.2
-
fructose 1,6-bisphosphate LDHB Rhizopus oryzae ATCC 9363
28.8
-
fructose 1,6-bisphosphate LDHB Rhizopus delemar