Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of an amino terminal fusion with a small ubiquitin-related modifier, SUMO, increases the oxidative activity per micromol protein by more than 100fold, while having little effect on the reductive LDH activity | Rhizopus delemar | |
additional information | presence of an amino terminal fusion with a small ubiquitin-related modifier, SUMO, increases the oxidative activity per micromol protein by more than 100fold, while having little effect on the reductive LDH activity | Rhizopus oryzae ATCC 9363 |
Cloned (Comment) | Organism |
---|---|
into pET-SUMO and expressed as 6× His Tag in Escherichia coli BL21 | Rhizopus delemar |
into pET-SUMO and expressed as 6× His Tag in Escherichia coli BL21 | Rhizopus oryzae ATCC 9363 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-bisphosphate | 10 mM significantly inhibits LDHB by 23% in a non-competitive manner. Level of inhibition seems to be even more pronounced at pH 6.2, compared to the optimal pH 6.8. At the more acidic pH and in the presence of 10 mM, LDHB shows a 42% decrease in activity | Rhizopus delemar | |
fructose 1,6-bisphosphate | 10 mM significantly inhibits LDHA abd LDHB (by 86%) in a non-competitive manner. Level of inhibition seems to be even more pronounced at pH 6.2, compared to the optimal pH 6.8. At the more acidic pH and in the presence of 10 mM, there is almost a 97% decrease in activity for LDHB | Rhizopus oryzae ATCC 9363 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
pyruvate | LDHB, in the presence of 0.25 mM NADH | Rhizopus oryzae ATCC 9363 | |
1.9 | - |
pyruvate | LDHB, in the presence of 0.2 mM NADH | Rhizopus oryzae ATCC 9363 | |
2.8 | - |
pyruvate | LDHB, in the presence of 0.15 mM NADH | Rhizopus oryzae ATCC 9363 | |
4.8 | - |
pyruvate | LDHB, in the presence of 0.125 mM NADH | Rhizopus oryzae ATCC 9363 | |
5 | - |
pyruvate | LDHB, in the presence of 0.25 mM NADH | Rhizopus delemar | |
7.7 | - |
pyruvate | LDHB, in the presence of 0.2 mM NADH | Rhizopus delemar | |
8.4 | - |
pyruvate | LDHB, in the presence of 0.15 mM NADH | Rhizopus delemar | |
15 | - |
pyruvate | LDHB, in the presence of 0.125 mM NADH | Rhizopus delemar | |
47.4 | - |
Lactate | LDHB, in the presence of 3.75 mM NAD+ | Rhizopus oryzae ATCC 9363 | |
105.1 | - |
Lactate | LDHB, in the presence of 3.75 mM NAD+ | Rhizopus delemar |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus delemar | - |
- |
- |
Rhizopus delemar RA 99-880 | - |
- |
- |
Rhizopus oryzae ATCC 9363 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on cobalt affinity resin | Rhizopus delemar |
on cobalt affinity resin | Rhizopus oryzae ATCC 9363 |
Storage Stability | Organism |
---|---|
4°C, glycerol and DDT containing buffer | Rhizopus delemar |
4°C, glycerol and DDT containing buffer | Rhizopus oryzae ATCC 9363 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Rhizopus delemar | lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Rhizopus oryzae ATCC 9363 | lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Rhizopus delemar RA 99-880 | lactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Rhizopus delemar |
lactate dehydrogenase | - |
Rhizopus oryzae ATCC 9363 |
LdhA | - |
Rhizopus oryzae ATCC 9363 |
LDHB | - |
Rhizopus delemar |
LDHB | - |
Rhizopus oryzae ATCC 9363 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
142 | - |
pyruvate | LDHB, in the presence of 0.125 mM NADH | Rhizopus delemar | |
154 | - |
pyruvate | LDHB, in the presence of 0.15 mM NADH | Rhizopus delemar | |
197 | - |
pyruvate | LDHB, in the presence of 0.125 mM NADH | Rhizopus oryzae ATCC 9363 | |
244 | - |
pyruvate | LDHB, in the presence of 0.15 mM NADH | Rhizopus oryzae ATCC 9363 | |
298 | - |
pyruvate | LDHB, in the presence of 0.2 mM NADH | Rhizopus delemar | |
318 | - |
pyruvate | LDHB, in the presence of 0.2 mM NADH | Rhizopus oryzae ATCC 9363 | |
320 | - |
pyruvate | RLDHB, in the presence of 0.25 mM NADH | Rhizopus oryzae ATCC 9363 | |
350 | - |
pyruvate | LDHB, in the presence of 0.25 mM NADH | Rhizopus delemar |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Rhizopus delemar |
6.8 | - |
- |
Rhizopus oryzae ATCC 9363 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.9 | LDHB, in Bis-Tris-propane buffer | Rhizopus delemar |
6.4 | 6.9 | LDHB, in Bis-Tris-propane buffer | Rhizopus oryzae ATCC 9363 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
fructose 1,6-bisphosphate | LDHA | Rhizopus oryzae ATCC 9363 | |
3.2 | - |
fructose 1,6-bisphosphate | LDHB | Rhizopus oryzae ATCC 9363 | |
28.8 | - |
fructose 1,6-bisphosphate | LDHB | Rhizopus delemar |