Cloned (Comment) | Organism |
---|---|
gene ldhA | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transposon mutagenesis of a reporter strain carrying a chromosomal ldhA promoter-lacZ fusion, PldhA-lacZ, using Tn5-based minitransposon system reveals that ldhA disruption drastically decreases expression of PldhA-lacZ. ldhA Promoter activity is reduced in the ldhA mutant. PldhA-lacZ expression in the ldhA mutant is restored by deletion of lldR, suggesting that LldR acts as a repressor of ldhA in the absence of L-lactate and the LldR-mediated repression is not relieved in the ldhA mutant due to its inability to produce L-lactate. lldR deletion does not affect PldhA-lacZ expression in the wild-type background during growth on either glucose, acetate, or L-lactate. However, it upregulates PldhA-lacZ expression in the sugR mutant background during growth on acetate. The binding sites of LldR and SugR are located around the -35 and -10 regions of the ldhA promoter, respectively | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Corynebacterium glutamicum | - |
pyruvate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene ldhA encoding L-lactate dehydrogenase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | - |
Corynebacterium glutamicum | pyruvate + NADH | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum | |
NADH | - |
Corynebacterium glutamicum |
Organism | Comment | Expression |
---|---|---|
Corynebacterium glutamicum | in the absence of sugar, SugR binds to the ldhA promoter region, thereby repressing ldhA expression. LldR also binds to the ldhA promoter region, thus negatively regulating ldhA expression. LldR is an L-lactate-responsive transcriptional repressor of L-lactate utilization genes | down |
General Information | Comment | Organism |
---|---|---|
additional information | ldhA expression is primarily repressed by SugR in the absence of sugar. In the presence of sugar, SugR-mediated repression of ldhA is alleviated, and ldhA expression is additionally enhanced by LldR inactivation in response to L-lactate produced by LdhA | Corynebacterium glutamicum |
physiological function | LADH-A is a key enzyme that couples L-lactate production to reoxidation of NADH formed during glycolysis | Corynebacterium glutamicum |