Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.27 extracted from

  • Zhou, Q.; Shao, W.L.
    Molecular genetic characterization of the thermostable L-lactate dehydrogenase gene (ldhL) of Thermoanaerobacter ethanolicus JW200 and biochemical characterization of the enzyme (2010), Biochemistry (Moscow), 75, 526-530.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 1,6-bisphosphate allosteric Thermoanaerobacter ethanolicus
Triton X-100 23% activation at 0.1% Thermoanaerobacter ethanolicus
Urea 9% activation at 0.1% Thermoanaerobacter ethanolicus

Cloned(Commentary)

Cloned (Comment) Organism
gene ldhL, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli Thermoanaerobacter ethanolicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10% inhibition at 1 mM Thermoanaerobacter ethanolicus
Ag+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
Cu2+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
DTT 19% inhibition at 1 mM Thermoanaerobacter ethanolicus
Fe2+ 74% inhibition at 1 mM Thermoanaerobacter ethanolicus
Fe3+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
NAD+ 7% inhibition at 0.5 mM Thermoanaerobacter ethanolicus
SDS complete inhibition at 0.1% Thermoanaerobacter ethanolicus
Zn2+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, the enzyme is allosteric in presence of D-fructose 1,6-bisphosphate, overview Thermoanaerobacter ethanolicus
0.18
-
pyruvate pH 5.8, 60°C, recombinant enzyme Thermoanaerobacter ethanolicus
60
-
L-lactate pH 7.0, 70°C, recombinant enzyme Thermoanaerobacter ethanolicus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not or poorly affected by 1 mM of EDTA, ATP, Mg2+, Co2+, Ca2+, Mn2+, and Ni2+ Thermoanaerobacter ethanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + NAD+ Thermoanaerobacter ethanolicus
-
pyruvate + NADH + H+
-
r
L-lactate + NAD+ Thermoanaerobacter ethanolicus JW 200
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus B1A4R6 gene ldhL
-
Thermoanaerobacter ethanolicus JW 200 B1A4R6 gene ldhL
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + NAD+
-
Thermoanaerobacter ethanolicus pyruvate + NADH + H+
-
r
L-lactate + NAD+
-
Thermoanaerobacter ethanolicus JW 200 pyruvate + NADH + H+
-
r

Subunits

Subunits Comment Organism
More TeLdhL contains a GXGXXG motif common to most NAD-linked dehydrogenases close to the N-terminus, and a putative signal peptide with cleavage site between residue 26 and 27, deletion of the putative signal peptide sequence leads to nonexpression of TeLdhL Thermoanaerobacter ethanolicus

Synonyms

Synonyms Comment Organism
L-(+)-lactate dehydrogenase
-
Thermoanaerobacter ethanolicus
L-LDH
-
Thermoanaerobacter ethanolicus
TeLdhL
-
Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
pyruvate reduction, recombinant enzyme Thermoanaerobacter ethanolicus
70
-
lactate oxidation, recombinant enzyme Thermoanaerobacter ethanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified recombinant enzyme, 1-5 h, pH 5.8-8.2, completely stable Thermoanaerobacter ethanolicus
80
-
purified recombinant enzyme, half-life is 1.5 h Thermoanaerobacter ethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
pyruvate reduction, recombinant enzyme Thermoanaerobacter ethanolicus
7
-
lactate oxidation, recombinant enzyme Thermoanaerobacter ethanolicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
incubation of TeLdhL at lower pH results in a rapid inactivation of the enzyme Thermoanaerobacter ethanolicus
5.8 8.2 purified recombinant enzyme, 1-5 h, 70°C, completely stable Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermoanaerobacter ethanolicus
NADH
-
Thermoanaerobacter ethanolicus