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Literature summary for 1.1.1.27 extracted from

  • Williams, R.A.; Andrews, P.
    Purification of the fructose 1,6-bisphosphate-dependent lactate dehydrogenase from Streptococcus uberis and an investigation of its existence in different forms (1986), Biochem. J., 236, 721-727.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate dependent on Streptococcus uberis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
NADH
-
Streptococcus uberis
0.23
-
NAD+
-
Streptococcus uberis
1.75
-
pyruvate
-
Streptococcus uberis
109
-
(S)-lactate
-
Streptococcus uberis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
4 * 35900, SDS-PAGE Streptococcus uberis
144000
-
equilibrium sedimentation Streptococcus uberis

Organism

Organism UniProt Comment Textmining
Streptococcus uberis
-
fructose 1,6-diphosphate dependent enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus uberis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1422
-
-
Streptococcus uberis

Storage Stability

Storage Stability Organism
-5°C, precipitated from phosphate buffer with (NH4)2SO4 and storage in the supernatant solution, stable for several weeks Streptococcus uberis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Streptococcus uberis pyruvate + NADH + H+
-
r
pyruvate + NADH + H+
-
Streptococcus uberis (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 35900, SDS-PAGE Streptococcus uberis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Streptococcus uberis

pH Range

pH Minimum pH Maximum Comment Organism
4.3 8 no activity below pH 4.3 and above pH 8.0 Streptococcus uberis

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Streptococcus uberis
NADH coenzyme Streptococcus uberis