Information on EC 1.1.1.27 - L-lactate dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.27
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RECOMMENDED NAME
GeneOntology No.
L-lactate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-lactate + NAD+ = pyruvate + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Bifidobacterium shunt
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Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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Cysteine and methionine metabolism
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Glycolysis / Gluconeogenesis
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heterolactic fermentation
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Metabolic pathways
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Microbial metabolism in diverse environments
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Propanoate metabolism
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pyruvate fermentation to lactate
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Pyruvate metabolism
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superpathway of glucose and xylose degradation
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L-lactaldehyde degradation
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lactate fermentation
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SYSTEMATIC NAME
IUBMB Comments
(S)-lactate:NAD+ oxidoreductase
Also oxidizes other (S)-2-hydroxymonocarboxylic acids. NADP+ also acts, more slowly, with the animal, but not the bacterial, enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-60-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Agama stellio stellio
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Manually annotated by BRENDA team
a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769
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Manually annotated by BRENDA team
a Gram-negative opportunistic pathogen found exclusively in the mammalian oral cavity in the space between the gums and the teeth known as the gingival crevice, gene AA02769
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Manually annotated by BRENDA team
Galapagos marine iguana, gene ldh-a
UniProt
Manually annotated by BRENDA team
epsilon-crystallin
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
strain 90
SwissProt
Manually annotated by BRENDA team
strain BS35, gene ldh
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Manually annotated by BRENDA team
L. Med
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene ldhA encoding L-lactate dehydrogenase
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
individuals from Man Island and mainland Wellington
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Manually annotated by BRENDA team
common iguana
UniProt
Manually annotated by BRENDA team
Lactobacillus mesenteroides
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Manually annotated by BRENDA team
strain SK007
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Manually annotated by BRENDA team
strain SK007
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Manually annotated by BRENDA team
strain 760
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Manually annotated by BRENDA team
strain MG1363, genes ldh and ldhB
Uniprot
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Molinema dessetae
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Manually annotated by BRENDA team
strain AKR
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Rubus idaeus
leaf
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Manually annotated by BRENDA team
no activity in Taraxacum officinale
leaf
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Manually annotated by BRENDA team
no activity in Zea mays
leaf
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
inhabiting the mantle cavity of host Pinna nobilis
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strains 99-880 and NRRL 395, genes ldhB and ldhA
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Manually annotated by BRENDA team
Rhizopus oryzae NRRL 395
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Streptococcus mitior
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GK24
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-lactate + 3-acetyl pyridine adenine dinucleotide
pyruvate + reduced 3-acetyl pyridine adenine dinucleotide
show the reaction diagram
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assay is based on reduction of 3-acetyl pyridine adenine dinucleotide that is specific for PfLDH, which allows the distinction of PfLDH from that of the host erythrocyte
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?
(S)-lactate + 3-acetylpyridine adenine dinucleotide
pyruvate + reduced 3-acetylpyridine adenine dinucleotide
show the reaction diagram
(S)-lactate + NAD(P)+
pyruvate + NAD(P)H + H+
show the reaction diagram
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
(S)-lactate + NADP+
pyruvate + NADPH + H+
show the reaction diagram
wild-type enzyme is specific for NAD+. Mutant enzyme F16Q/I37K/D38SC81S/N85R utilizes NADP+ better than wild-type enzyme, prefers NADP+ to NAD+. Mutant F16Q/C81S/N85R utilizes NAD+ better than wild-type enzyme, weakly active with NADP+
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?
2-oxobutyrate + NADH
2-hydroxybutyrate + NAD+
show the reaction diagram
2-oxoglutarate + NADH + H+
2-hydroxyglutarate + NAD+
show the reaction diagram
2-oxopentanoate + NADH
2-hydroxypentanoate + NAD+
show the reaction diagram
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125% of the activity with pyruvate
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?
2-oxovalerate + NADH
2-hydroxyvalerate + NAD+
show the reaction diagram
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r
3-fluoropyruvate + NADH
?
show the reaction diagram
3-methyl-2-oxobutanoate + NADH
2-hydroxy-3-methylbutanoate + NAD+
show the reaction diagram
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28.5% of the activity with pyruvate
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?
3-methyl-2-oxopentanoate + NADH
2-hydroxy-3-methylpentanoate + NAD+
show the reaction diagram
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5.3% of the activity with pyruvate
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?
4-methyl-2-oxopentanoate + NADH
2-hydroxy-4-methylpentanoate + NAD+
show the reaction diagram
bromopyruvate + NADH
5-bromo-2-hydroxypropanoate + NAD+
show the reaction diagram
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?
glyoxylate + NADH
glycolate + NAD+
show the reaction diagram
hydroxypyruvate + NADPH + H+
2,3-dihydroxypropanoate + NADP+
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
oxamate + NADH
?
show the reaction diagram
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two distinct active site LDH/NADH-oxamate complex conformations, a major populated structure wherein all significant hydrogen-bonding patterns are formed at the active site between protein and bound ligand necessary for the catalytically productive Michaelis complex and, a minor structure in a configuration of the active site that is unfavorable to carry out catalyzed chemistry. This latter structure likely simulates a dead-end complex in the reaction mixture. The evolution of the encounter complex between LDH/NADH and oxamate collapses via a branched reaction pathway to form the major and minor bound species. Once the encounter complex is formed between LDH/NADH and substrate, the ternary protein-ligand complex appears to fold to form a compact productive complex in an all or nothing like fashion with all the important molecular interactions coming together at the same time
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?
phenylpyruvate + NADH
2-hydroxy-3-phenylpropanoate + NAD+
show the reaction diagram
phenylpyruvate + NADH
phenyllactate + NAD+
show the reaction diagram
pyruvate + NADH
?
show the reaction diagram
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?
pyruvate + NADH
L-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
lactate + NAD+
show the reaction diagram
pyruvate + NADPH + H+
(S)-lactate + NADP+
show the reaction diagram
pyruvate ethyl ester + NADH
2-hydroxypropanoate ethyl ester
show the reaction diagram
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?
pyruvate methyl ester + NADH
2-hydroxypropanoate methyl ester
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-lactate + NAD(P)+
pyruvate + NAD(P)H + H+
show the reaction diagram
(S)-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH
show the reaction diagram
L-lactate + NAD+
pyruvate + NADH + H+
show the reaction diagram
phenylpyruvate + NADH
phenyllactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
(S)-lactate + NAD+
show the reaction diagram
pyruvate + NADH + H+
L-lactate + NAD+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-acetylpyridine adenine dinucleotide
beta-NAD+
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beta-NADH
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FMN
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contains non-covalently bound FMN as prosthetic group
NADP+
NADPH
nicotinamide hypoxanthine dinucleotide
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alternative coenzyme
thio-NAD+
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alternative coenzyme
additional information
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the enzyme also utilizes both NAD(H) and NADP(H)
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