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Literature summary for 1.1.1.27 extracted from

  • Romero, S.; Merino, E.; Bolivar, F.; Gosset, G.; Martinez, A.
    Metabolic engineering of Bacillus subtilis for ethanol production: lactate dehydrogenase plays a key role in fermentative metabolism (2007), Appl. Environ. Microbiol., 73, 5190-5198.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ldh Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information construct an ethanologenic Bacillus subtilis strain, the ldh gene is disrupted in strain WB700 by chromosomal insertion of the Zymomonas mobilis pyruvate decarboxylase gene pdc and the alcohol dehydrogenase II gene adhB under the control of the ldh native promoter leading to production of etahnol and butanediol by the recombinant strain. But cell growth and glucose consumption rates in strain BS35 are reduced by 70 and 65%, respectively, in comparison to the progenitor strain. To eliminate butanediol production, the acetolactate synthase gene alsS is inactivated resulting in BS36, additional expression of transhydrogenase encoded by gene udhA from Escherichia coli allows a partial recovery of the cell growth rate and an early onset of ethanol production resulting in strain BS37, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD(P)+ Bacillus subtilis
-
pyruvate + NAD(P)H + H+
-
r
(S)-lactate + NAD(P)+ Bacillus subtilis BS35
-
pyruvate + NAD(P)H + H+
-
r
additional information Bacillus subtilis Bacillus subtilis fermentation pathways, overview ?
-
?
additional information Bacillus subtilis BS35 Bacillus subtilis fermentation pathways, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene ldh
-
Bacillus subtilis BS35
-
gene ldh
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
cofactor specificity in different strains Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD(P)+
-
Bacillus subtilis pyruvate + NAD(P)H + H+
-
r
(S)-lactate + NAD(P)+
-
Bacillus subtilis BS35 pyruvate + NAD(P)H + H+
-
r
additional information Bacillus subtilis fermentation pathways, overview Bacillus subtilis ?
-
?
additional information the enzyme also utilizes NADP(H) Bacillus subtilis ?
-
?
additional information Bacillus subtilis fermentation pathways, overview Bacillus subtilis BS35 ?
-
?
additional information the enzyme also utilizes NADP(H) Bacillus subtilis BS35 ?
-
?

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
Bacillus subtilis
LDH
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme also utilizes both NAD(H) and NADP(H) Bacillus subtilis
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis
NADP+
-
Bacillus subtilis
NADPH
-
Bacillus subtilis