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Literature summary for 1.1.1.27 extracted from

  • Zhang, Y.; Gao, X.
    Expression, purification, crystallization and preliminary X-ray crystallographic analysis of L-lactate dehydrogenase and its H171C mutant from Bacillus subtilis (2012), Acta Crystallogr. Sect. F, 68, 63-65.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified His-tagged wild-type and mutant H171C enzymes, hanging drop vapor diffusion method, 0.002 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 8.5, with 1 mM fructose 1,6-bisphosphate and NAD+, is mixed with 0.002 ml of reservoir solution containing 16% w/v PEG MME 2000, 0.06 M sodium/potassium phosphate, 1.8% v/v glycerol pH 5.5, or 14% w/v PEG 4000, 0.1 M sodium/potassium phosphate, 2% v/v glycerol, pH 7.0, equilibration against 1 ml reservoir solution, X-ray diffraction structure determinaation and analysis at 2.2-2.5 A resolution Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
H171C site-directed mutagenesis Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Bacillus subtilis
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Bacillus subtilis pyruvate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
LDH
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis

General Information

General Information Comment Organism
metabolism L-lactate dehydrogenase is an important enzyme involved in the last step of glycolysis that catalyzes the reversible conversion of pyruvate to L-lactate with the simultaneous oxidation of NADH to NAD+ Bacillus subtilis