Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.27 extracted from

  • Hillier, A.J.; Jago, G.R.
    L-Lactate dehydrogenase, FDP-activated, from Streptococcus cremoris (1982), Methods Enzymol., 89, 362-367.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Lactococcus cremoris
glucose 1,6-diphosphate activates Lactococcus cremoris
tagatose 1,6-diphosphate stimulates Lactococcus cremoris

General Stability

General Stability Organism
NADH and fructose 1,6-diphosphate partially stabilize the 140000 Da molecular weight form against dissociation in triethanolamine-hydrochloride buffer at pH 8.0 Lactococcus cremoris

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Lactococcus cremoris
oxamate
-
Lactococcus cremoris
p-chloromercuribenzoate
-
Lactococcus cremoris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
NADH in presence of fructose 1,6-diphosphate Lactococcus cremoris
1.15
-
pyruvate in absence of fructose 1,6-diphosphate Lactococcus cremoris
7.7
-
pyruvate in presence of fructose 1,6-diphosphate Lactococcus cremoris
44
-
NADH in absence of fructose 1,6-diphosphate Lactococcus cremoris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
ultracentrifugal studies Lactococcus cremoris

Organism

Organism UniProt Comment Textmining
Lactococcus cremoris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus cremoris

Reaction

Reaction Comment Organism Reaction ID
(S)-lactate + NAD+ = pyruvate + NADH + H+ reduction of pyruvate follows an Theorell-Chance mechanism Lactococcus cremoris

Storage Stability

Storage Stability Organism
-20°C, 50 mM sodium phosphate buffer, pH 7.0, stable for several months Lactococcus cremoris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+
-
Lactococcus cremoris (S)-lactate + NAD+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
protection against heat inactivation by 1 mM fructose 1,6-diphosphate, 1 mM ATP, 1 mM glucose 6-phosphate and increased levels of phosphate Lactococcus cremoris

Cofactor

Cofactor Comment Organism Structure
NADH coenzyme Lactococcus cremoris