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Literature summary for 1.1.1.27 extracted from

  • Li, X.; Jiang, B.; Pan, B.; Mu, W.; Zhang, T.
    Purification and partial characterization of Lactobacillus species SK007 lactate dehydrogenase (LDH) catalyzing phenylpyruvic acid (PPA) conversion into phenyllactic acid (PLA) (2008), J. Agric. Food Chem., 56, 2392-2399.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme might be useful in the production of phenyllactate Lactobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
pyruvate pH 6.5, 30°C, purified enzyme Lactobacillus sp.
1.76
-
phenylpyruvate pH 6.5, 30°C, purified enzyme Lactobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
2 * 39000, SDS-PAGE Lactobacillus sp.
78000
-
gel filtration Lactobacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Lactobacillus sp.
-
pyruvate + NADH + H+
-
r
(S)-lactate + NAD+ Lactobacillus sp. SK007
-
pyruvate + NADH + H+
-
r
phenylpyruvate + NADH Lactobacillus sp.
-
phenyllactate + NAD+
-
r
phenylpyruvate + NADH Lactobacillus sp. SK007
-
phenyllactate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Lactobacillus sp.
-
-
-
Lactobacillus sp. SK007
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 34.3fold to about 98% purity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Lactobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
203.3
-
purified native enzyme, substrate pyruvate Lactobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Lactobacillus sp. pyruvate + NADH + H+
-
r
(S)-lactate + NAD+
-
Lactobacillus sp. SK007 pyruvate + NADH + H+
-
r
phenylpyruvate + NADH
-
Lactobacillus sp. phenyllactate + NAD+
-
r
phenylpyruvate + NADH
-
Lactobacillus sp. SK007 phenyllactate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Lactobacillus sp.

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
Lactobacillus sp.
LDH
-
Lactobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
with substrate phenylpyruvate Lactobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 90% of maximal activity at 50°C and 50% at 60°C Lactobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
with substrate phenylpyruvate Lactobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 9 activity range, the enzyme is inactive below pH 3.0 and above pH 9.0 Lactobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 rather stable from pH 4.0 to pH 8.0, but rapid decline in activity below pH 4.0 and above pH 8.0 Lactobacillus sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lactobacillus sp.
NADH
-
Lactobacillus sp.