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Literature summary for 1.1.1.27 extracted from

  • Al-Jassabi, S.
    Purification and kinetic properties of skeletal muscle lactate dehydrogenase from the lizard Agama stellio stellio (2002), Biochemistry, 67, 786-789.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ both directions Agama stellio stellio
Cu2+ both directions Agama stellio stellio
glutamate both directions Agama stellio stellio
Mg2+ both directions Agama stellio stellio
Mn2+ both directions Agama stellio stellio
oxalate both directions Agama stellio stellio

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NAD+ pH 7.5, 25°C Agama stellio stellio
0.02
-
pyruvate pH 7.5, 25°C Agama stellio stellio
0.04
-
NADH pH 7.5, 25°C Agama stellio stellio
8.1
-
(S)-lactate pH 7.5, 25°C Agama stellio stellio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE Agama stellio stellio

Organism

Organism UniProt Comment Textmining
Agama stellio stellio
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme LDH-5 Agama stellio stellio

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Agama stellio stellio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
-
Agama stellio stellio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+
-
Agama stellio stellio (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Agama stellio stellio

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
30 min, stable Agama stellio stellio
90
-
rapid inactivation Agama stellio stellio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
with pyruvate as substrate Agama stellio stellio
9.2
-
with lactate as substrate Agama stellio stellio

pH Range

pH Minimum pH Maximum Comment Organism
5.4 10.8 pH 5.4: about 60% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with pyruvate Agama stellio stellio
6.6 10.8 pH 5.4: about 55% of maximal activity, pH 10.8: about 55% of maximal activity, reaction with lactate Agama stellio stellio

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Agama stellio stellio
NADH
-
Agama stellio stellio