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Literature summary for 1.1.1.27 extracted from

  • Dong, J.B.; Liu, X.; Li, L.F.; Wu, S.; Su, X.D.
    Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 65, 1053-1055.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development Sa-LDH-1 can become a potential drug target for antibiotics against Staphylococcus aureus Staphylococcus aureus

Cloned(Commentary)

Cloned (Comment) Organism
gene ldh-1, expression of N-termially His6-tagged enzyme in Escherichia coli BL21 Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged Sa-LDH-1, sitting drop vapour diffusion method, 0.001 ml protein solution, containing 5 mg/ml protein in 20 mM Tris-HCl, 200 mM NaCl, pH 7.5, is mixed with mixed with an equal volume of reservoir solution, containing ,0.2 M calcium chloride dihydrate, 0.1 M Na HEPES, pH 7.5, 28%(v/v) PEG 400, and equilibrated against 0.1 ml reservoir solution, 3 days, X-ray diffraction structure determination and analysis at 2.4 A resolution Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34600
-
x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus
39000
-
x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + NAD+ Staphylococcus aureus
-
pyruvate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
gene ldh-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-termially His6-tagged LDH-1 from Escherichia coli BL21 by nickel affinity chromatography and gel filtration to homogeneity Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + NAD+
-
Staphylococcus aureus pyruvate + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation Staphylococcus aureus

Synonyms

Synonyms Comment Organism
More lactate dehydrogenase is a member of the 2-hydroxyacid oxidoreductase family Staphylococcus aureus
nitric oxideinducible l-lactate dehydrogenase
-
Staphylococcus aureus
Sa-LDH-1
-
Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Staphylococcus aureus

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the stereospecific conversion of lactate to pyruvate and converts NAD+ to NADH, which is an important way of regenerating NAD+, enabling the continuation of glycolysis Staphylococcus aureus