Application | Comment | Organism |
---|---|---|
drug development | Sa-LDH-1 can become a potential drug target for antibiotics against Staphylococcus aureus | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
gene ldh-1, expression of N-termially His6-tagged enzyme in Escherichia coli BL21 | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged Sa-LDH-1, sitting drop vapour diffusion method, 0.001 ml protein solution, containing 5 mg/ml protein in 20 mM Tris-HCl, 200 mM NaCl, pH 7.5, is mixed with mixed with an equal volume of reservoir solution, containing ,0.2 M calcium chloride dihydrate, 0.1 M Na HEPES, pH 7.5, 28%(v/v) PEG 400, and equilibrated against 0.1 ml reservoir solution, 3 days, X-ray diffraction structure determination and analysis at 2.4 A resolution | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34600 | - |
x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation | Staphylococcus aureus |
39000 | - |
x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Staphylococcus aureus | - |
pyruvate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene ldh-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-termially His6-tagged LDH-1 from Escherichia coli BL21 by nickel affinity chromatography and gel filtration to homogeneity | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | - |
Staphylococcus aureus | pyruvate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, recombinant His6-tagged LDH-1, SDS-PAGE, x * 34600, about, sequence calculation | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
More | lactate dehydrogenase is a member of the 2-hydroxyacid oxidoreductase family | Staphylococcus aureus |
nitric oxideinducible l-lactate dehydrogenase | - |
Staphylococcus aureus |
Sa-LDH-1 | - |
Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the stereospecific conversion of lactate to pyruvate and converts NAD+ to NADH, which is an important way of regenerating NAD+, enabling the continuation of glycolysis | Staphylococcus aureus |