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Literature summary for 1.1.1.27 extracted from

  • Liwak, U.; Ananvoranich, S.
    Toxoplasma gondii: Over-expression of lactate dehydrogenase enhances differentiation under alkaline conditions (2009), Exp. Parasitol., 122, 155-161.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into expression plasmid pTUB8Myc-His-X-HX under the control of a modified tubulin promoter. Type I strain of Toxoplasma gondii RHDELTAHX transformed with plasmid. Transgenic parasites overexpressing LDH1 Toxoplasma gondii
into expression plasmid pTUB8Myc-His-X-HX under the control of a modified tubulin promoter. Type I strain of Toxoplasma gondii RHDELTAHX transformed with plasmid. Transgenic parasites overexpressing LDH2 Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Toxoplasma gondii 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
antibodies Toxoplasma gondii
39900
-
antibodies Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii P90613
-
-
Toxoplasma gondii Q27797
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bradyzoite LDH2 Toxoplasma gondii
-
additional information LDH1 is absent from bradyzoites Toxoplasma gondii
-
additional information LDH2 is absent from tachyzoites Toxoplasma gondii
-
tachyzoite
-
Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH LDH1 has an important physiological function, in addition to being a glycolytic enzyme and differentiation marker. The enzymatic activity, growth, and virulence of tachyzoites are unaffected by the presence of the recombinant protein. Overexpression of LDH1 enhances the parasite's ability to differentiate Toxoplasma gondii L-lactate + NAD+
-
?
pyruvate + NADH LDH2 has an important physiological function, in addition to being a glycolytic enzyme and differentiation marker. The enzymatic activity, growth, and virulence of tachyzoites are unaffected by the presence of the recombinant protein. Overexpression of LDH2 enhances the parasite's ability to differentiate Toxoplasma gondii L-lactate + NAD+
-
?

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
Toxoplasma gondii
LDH1
-
Toxoplasma gondii
LDH2
-
Toxoplasma gondii

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
under alkaline conditions the presence of the recombinant LDH protein increases the differentiation Toxoplasma gondii
additional information
-
under alkaline conditions the presence of the recombinant LDH proteins increases the differentiation Toxoplasma gondii