Cloned (Comment) | Organism |
---|---|
expressed in Leuconostoc citreum using a shuttle vector pLeuCM, change of the pyruvate carbon flux in Leuconostoc citreum from D-lactate into L-lactate by heterologous expression of L-lactate dehydrogenase | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
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Lactiplantibacillus plantarum | P56512 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH | in the cell lysate of the host strain, L-LDH activity is hardly detectable during cultivation. As a consequence of transformation, the ratio between D- and L-isomers is changed due to the increment of L-lactate and the decrement of D-lactate, but there are no significant differences in total lactate concentration between the host and transformant cells. In the transformant harboring pLC18lld, L-LDH activity is detected during the entire cultivation period. L-LDH activity increases rapidly to the maximum level (0.18 U/mg protein) between the early- and mid-exponential growth phases (0-8 h), and decreases thereafter during the stationary phase | Lactiplantibacillus plantarum | L-lactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-LDH | - |
Lactiplantibacillus plantarum |