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Literature summary for 1.1.1.27 extracted from

  • Jin, Q.; Jung, J.Y.; Kim, Y.J.; Eom, H.J.; Kim, S.Y.; Kim, T.J.; Han, N.S.
    Production of l-lactate in Leuconostoc citreum via heterologous expression of l-lactate dehydrogenase gene (2009), J. Biotechnol., 144, 160-164.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Leuconostoc citreum using a shuttle vector pLeuCM, change of the pyruvate carbon flux in Leuconostoc citreum from D-lactate into L-lactate by heterologous expression of L-lactate dehydrogenase Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum P56512
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH in the cell lysate of the host strain, L-LDH activity is hardly detectable during cultivation. As a consequence of transformation, the ratio between D- and L-isomers is changed due to the increment of L-lactate and the decrement of D-lactate, but there are no significant differences in total lactate concentration between the host and transformant cells. In the transformant harboring pLC18lld, L-LDH activity is detected during the entire cultivation period. L-LDH activity increases rapidly to the maximum level (0.18 U/mg protein) between the early- and mid-exponential growth phases (0-8 h), and decreases thereafter during the stationary phase Lactiplantibacillus plantarum L-lactate + NAD+
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Synonyms

Synonyms Comment Organism
L-LDH
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Lactiplantibacillus plantarum