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Literature summary for 1.1.1.27 extracted from

  • Yin, Y.; Kirsch, J.F.
    Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase (2007), Proc. Natl. Acad. Sci. USA, 104, 17353-17357.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information the malate dehydrogenase, EC 1.1.1.37, mutant I12V/R81Q/M85E/G210A/V214I shows a substrate specificity that is switched from malate dehydrogenase to that of lactate dehydrogenase, overview Escherichia coli
additional information the malate dehydrogenase, EC 1.1.1.37, mutant I12V/R81Q/M85E/G210A/V214I shows a substrate specificity that is switched from malate dehydrogenase to that of lactate dehydrogenase, overview Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
pyruvate pH 6.0, 25°C, recombinant wild-type enzyme Geobacillus stearothermophilus
13.3
-
pyruvate pH 7.5, 25°C, recombinant mutant I12V/R81Q/M85E/G210A/V214I Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Geobacillus stearothermophilus
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Geobacillus stearothermophilus P00344 gene ldh
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Geobacillus stearothermophilus pyruvate + NADH + H+
-
r
(S)-lactate + NAD+ mutant I12V/R81Q/M85E/G210A/V214I, residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity Escherichia coli pyruvate + NADH + H+
-
r
(S)-lactate + NAD+ residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity Geobacillus stearothermophilus pyruvate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
Escherichia coli
lactate dehydrogenase
-
Geobacillus stearothermophilus
LDH
-
Escherichia coli
LDH
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Geobacillus stearothermophilus
30
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46.5
-
pyruvate pH 7.5, 25°C, recombinant mutant I12V/R81Q/M85E/G210A/V214I Escherichia coli
250
-
pyruvate pH 6.0, 25°C, recombinant wild-type enzyme Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Geobacillus stearothermophilus
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NAD+
-
Geobacillus stearothermophilus
NADH
-
Escherichia coli
NADH
-
Geobacillus stearothermophilus