Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.27 extracted from

  • Chang, G.G.; Huang, S.M.; Chiou, S.H.
    Kinetic mechanism of the endogenous lactate dehydrogenase activity of duck epsilon-crystallin (1991), Arch. Biochem. Biophys., 284, 285-291.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-Aminopyridine adenine dinucleotide competitive versus NAD+ and noncompetitive versus L-lactate Anas platyrhynchos
Alpha-NAD+ noncompetitive inhibitor versus beta-NAD+ Anas platyrhynchos
D-lactate dead-end inhibitor, competitive inhibitor versus L-lactate Anas platyrhynchos
L-lactate substrate inhibition is uncompetitive Anas platyrhynchos
nicotinic acid adenine dinucleotide competitive versus NAD+ and noncompetitive versus L-lactate Anas platyrhynchos
oxamate dead-end inhibitor, competitive inhibitor versus pyruvate Anas platyrhynchos
pyruvate substrate inhibition is uncompetitive Anas platyrhynchos
Tartronate dead-end inhibitor, competitive inhibitor versus L-lactate Anas platyrhynchos
Thionicotinamide adenine dinucleotide competitive versus NAD+ and noncompetitive versus L-lactate Anas platyrhynchos

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos
-
epsilon-crystallin
-
Gallus gallus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-lactate + NAD+ = pyruvate + NADH + H+ mechanism Anas platyrhynchos

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Gallus gallus
-
muscle
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 3-acetylpyridine adenine dinucleotide
-
Gallus gallus pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
r
(S)-lactate + 3-acetylpyridine adenine dinucleotide
-
Anas platyrhynchos pyruvate + reduced 3-acetylpyridine adenine dinucleotide
-
r
(S)-lactate + epsilon-NAD+
-
Anas platyrhynchos pyruvate + epsilon-NADH + H+
-
r
(S)-lactate + nicotinamide hypoxanthine dinucleotide
-
Gallus gallus pyruvate + reduced nicotinamide hypoxanthine dinucleotide
-
r
(S)-lactate + nicotinamide hypoxanthine dinucleotide
-
Anas platyrhynchos pyruvate + reduced nicotinamide hypoxanthine dinucleotide
-
r
3-fluoropyruvate + NADH
-
Gallus gallus ?
-
?
3-fluoropyruvate + NADH
-
Anas platyrhynchos ?
-
?
bromopyruvate + NADH
-
Anas platyrhynchos 3-bromo-2-hydroxypropanoate + NAD+
-
?
pyruvate + NADH + H+
-
Gallus gallus (S)-lactate + NAD+
-
?
pyruvate + NADH + H+
-
Anas platyrhynchos (S)-lactate + NAD+
-
r
pyruvate ethyl ester + NADH
-
Anas platyrhynchos 2-hydroxypropanoate ethyl ester
-
?
pyruvate methyl ester + NADH
-
Anas platyrhynchos 2-hydroxypropanoate methyl ester
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
epsilon-NAD+
-
Anas platyrhynchos
1.1
-
nicotinamide hypoxanthine dinucleotide
-
Anas platyrhynchos
1.1
-
nicotinamide hypoxanthine dinucleotide enzyme from muscle Gallus gallus
1.4
-
3-acetylpyridine adenine dinucleotide
-
Anas platyrhynchos
3.3
-
NAD+ enzyme from muscle Gallus gallus
5.3
-
epsilon-NAD+
-
Anas platyrhynchos
5.5
-
nicotinamide hypoxanthine dinucleotide
-
Anas platyrhynchos
6.2
-
epsilon-NAD+ enzyme from heart Anas platyrhynchos
6.9
-
NAD+
-
Anas platyrhynchos
16
-
3-acetylpyridine adenine dinucleotide enzyme form heart and muscle Gallus gallus
18.8
-
nicotinamide hypoxanthine dinucleotide enzyme from heart Anas platyrhynchos
60
-
NAD+ enzyme from heart Gallus gallus
98
-
Fluoropyruvate
-
Gallus gallus
176
-
pyruvate ethyl ester
-
Anas platyrhynchos
204
-
pyruvate
-
Anas platyrhynchos
227
-
pyruvate methyl ester
-
Anas platyrhynchos
269
-
Bromopyruvate
-
Anas platyrhynchos

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine adenine dinucleotide alternative coenzyme Anas platyrhynchos
NADH coenzyme Gallus gallus
nicotinamide hypoxanthine dinucleotide alternative coenzyme Anas platyrhynchos
thio-NAD+ alternative coenzyme Anas platyrhynchos