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Literature summary for 1.1.1.27 extracted from

  • Cripps, R.E.; Eley, K.; Leak, D.J.; Rudd, B.; Taylor, M.; Todd, M.; Boakes, S.; Martin, S.; Atkinson, T.
    Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production (2009), Metab. Eng., 11, 398-408.
    View publication on PubMed

Application

Application Comment Organism
synthesis metabolic engineering of Geobacillus thermoglucosidasius to divert the fermentative carbon flux from a mixed acid pathway, to one in which ethanol becomes the major product, involving elimination of the lactate dehydrogenase and pyruvate formate lyase pathways by disruption of the ldh and pflB genes, respectively, and upregulation of expression of pyruvate dehydrogenase. Strains with all three modifications form ethanol efficiently and rapidly at temperatures in excess of 60°C in yields in excess of 90% of theoretical. The strains also efficiently ferment cellobiose and a mixed hexose and pentose feed Parageobacillus thermoglucosidasius

Cloned(Commentary)

Cloned (Comment) Organism
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Parageobacillus thermoglucosidasius

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius
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Parageobacillus thermoglucosidasius NCIMB 11955
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Synonyms

Synonyms Comment Organism
LDH
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Parageobacillus thermoglucosidasius

General Information

General Information Comment Organism
physiological function Inactivation of the lactate dehydrogenase gene results in a metabolic shift predominantly towards ethanol production. Formic and acetic acids are still produced, the latter in lower amounts than with the wild-type, but, unlike the wild-type strain, significant quantities of pyruvic acid accumulate Parageobacillus thermoglucosidasius