Protein Variants | Comment | Organism |
---|---|---|
R157L | mutation appears to deregulate activation by fructose 1,6-bisphosphate, leading to constitutive activation of lactate dehydrogenase | Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | I3VSF1 | - |
- |
Thermoanaerobacterium saccharolyticum DSM 8691 | I3VSF1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
wild-type, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate | Thermoanaerobacterium saccharolyticum |
0.58 | - |
mutant R157L, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate | Thermoanaerobacterium saccharolyticum |
0.63 | - |
mutant R157L, pH 7.3, 55°C | Thermoanaerobacterium saccharolyticum |
0.65 | - |
wild-type, pH 7.3, 55°C | Thermoanaerobacterium saccharolyticum |
1.27 | - |
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate | Thermoanaerobacterium saccharolyticum |
1.44 | - |
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum DSM 8691 | (S)-lactate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdhE | - |
Thermoanaerobacterium saccharolyticum |
AdhE | bifunctional alcohol and aldehyde dehydrogenase gene | Thermoanaerobacterium saccharolyticum |
Tsac_0416 | - |
Thermoanaerobacterium saccharolyticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Thermoanaerobacterium saccharolyticum |