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Literature summary for 1.1.1.27 extracted from

  • Lo, J.; Zheng, T.; Hon, S.; Olson, D.G.; Lynd, L.R.
    The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum (2015), J. Bacteriol., 197, 1386-1393.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
R157L mutation appears to deregulate activation by fructose 1,6-bisphosphate, leading to constitutive activation of lactate dehydrogenase Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum I3VSF1
-
-
Thermoanaerobacterium saccharolyticum DSM 8691 I3VSF1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.11
-
wild-type, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate Thermoanaerobacterium saccharolyticum
0.58
-
mutant R157L, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate Thermoanaerobacterium saccharolyticum
0.63
-
mutant R157L, pH 7.3, 55°C Thermoanaerobacterium saccharolyticum
0.65
-
wild-type, pH 7.3, 55°C Thermoanaerobacterium saccharolyticum
1.27
-
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C, presence of fructose-1,6-bisphosphate Thermoanaerobacterium saccharolyticum
1.44
-
mutant R157L, lacking alcohol/aldehyde dehydrogenase gene AdhE, pH 7.3, 55°C Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + NADH + H+
-
Thermoanaerobacterium saccharolyticum (S)-lactate + NAD+
-
?
pyruvate + NADH + H+
-
Thermoanaerobacterium saccharolyticum DSM 8691 (S)-lactate + NAD+
-
?

Synonyms

Synonyms Comment Organism
AdhE
-
Thermoanaerobacterium saccharolyticum
AdhE bifunctional alcohol and aldehyde dehydrogenase gene Thermoanaerobacterium saccharolyticum
Tsac_0416
-
Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Thermoanaerobacterium saccharolyticum