Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as maltose-binding protein fusion protein. CpLDH1 is probably evolved from the same ancestor of CpMalDH1 by a very recent gene duplication that occurs after Cryptosporidium parvum diverges from other apocomplexans | Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
pyruvate | pH 8.0, 25°C | Cryptosporidium parvum | |
0.5 | - |
NADH | pH 8.0, 25°C | Cryptosporidium parvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
monomer, sedimentation velocity | Cryptosporidium parvum |
134000 | - |
dimer, sedimentation velocity | Cryptosporidium parvum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | Q9GT92 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Cryptosporidium parvum | (S)-lactate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 77000, monomer-dimer equilibrium in solution | Cryptosporidium parvum |
monomer | 1 * 77000, monomer-dimer equilibrium in solution | Cryptosporidium parvum |
Synonyms | Comment | Organism |
---|---|---|
LDH | - |
Cryptosporidium parvum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cryptosporidium parvum |