Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
pyruvate | octameric enzyme form | Thermotoga maritima | |
0.019 | - |
pyruvate | tetrameric enzyme form | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
8 * 35000, enzyme also exists in an tetrameric enzyme form, SDS-PAGE, meniscus depletion experiments in 4 M guanidinium chloride | Thermotoga maritima |
140000 | - |
tetramer, gel filtration | Thermotoga maritima |
269300 | - |
meniscus depletion experiments | Thermotoga maritima |
280000 | - |
octamer, gel filtration | Thermotoga maritima |
280900 | - |
equilibrium sedimentation | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Thermotoga maritima | (S)-lactate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 35000, enzyme also exists in an tetrameric enzyme form, SDS-PAGE, meniscus depletion experiments in 4 M guanidinium chloride | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | coenzyme | Thermotoga maritima |