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Literature summary for 1.1.1.27 extracted from

  • Ilmen, M.; Koivuranta, K.; Ruohonen, L.; Suominen, P.; Penttila, M.
    Efficient production of L-lactic acid from xylose by Pichia stipitis (2007), Appl. Environ. Microbiol., 73, 117-123.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis development of yeast-based bioprocesses to produce lactate from lignocellulosic raw material Lactobacillus helveticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia stipidis strain CBS6054, subcloning in Escherichia coli strain DH5alpha Lactobacillus helveticus

Protein Variants

Protein Variants Comment Organism
additional information construction of a Pichia stipitis strain CBS6054, that expresses the LDH from Lactobacillus helveticus under the control of the Pichia stipitis fermentative ADH1 promoter, the mutant yeast uses xylose, glucose, or a mixture of the two sugars as the carbon source for lactate production, LDH competes efficiently with the ethanol pathway for pyruvate, overview Lactobacillus helveticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Lactobacillus helveticus
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
gene ldhL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Lactobacillus helveticus pyruvate + NADH + H+
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Lactobacillus helveticus
NADH
-
Lactobacillus helveticus