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Literature summary for 1.1.1.27 extracted from

  • Savijoki, K.; Palva, A.
    Molecular genetic characterization of the L-lactate dehydrogenase gene (ldhL) of Lactobacillus helveticus and biochemical characterization of the enzyme (1997), Appl. Environ. Microbiol., 63, 2850-2856.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Lactobacillus helveticus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
Co2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
Cu2+ 0.1 mM and 1.0 mM, weak inhibition Lactobacillus helveticus
HEPES buffer
-
Lactobacillus helveticus
MES buffer
-
Lactobacillus helveticus
p-hydroxymercuribenzoate
-
Lactobacillus helveticus
Tris-HCl
-
Lactobacillus helveticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
pyruvate
-
Lactobacillus helveticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, almost 2fold stimulation Lactobacillus helveticus
Zn2+ 1 mM, almost 2fold stimulation Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000 138000 gel filtration Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2119
-
recombinant enzyme Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH 3% of the activity with pyruvate Lactobacillus helveticus 2-hydroxybutyrate + NAD+
-
?
phenylpyruvate + NADH 28% of the activity with pyruvate Lactobacillus helveticus 2-hydroxy-3-phenylpropanoate + NAD+
-
?
pyruvate + NADH + H+
-
Lactobacillus helveticus (S)-lactate + NAD+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
10 min, 90% loss of activity Lactobacillus helveticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
643
-
pyruvate
-
Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Lactobacillus helveticus

Cofactor

Cofactor Comment Organism Structure
NADH coenzyme Lactobacillus helveticus