Cloned (Comment) | Organism |
---|---|
- |
Lactobacillus helveticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.1 mM and 1.0 mM, weak inhibition | Lactobacillus helveticus | |
Co2+ | 0.1 mM and 1.0 mM, weak inhibition | Lactobacillus helveticus | |
Cu2+ | 0.1 mM and 1.0 mM, weak inhibition | Lactobacillus helveticus | |
HEPES buffer | - |
Lactobacillus helveticus | |
MES buffer | - |
Lactobacillus helveticus | |
p-hydroxymercuribenzoate | - |
Lactobacillus helveticus | |
Tris-HCl | - |
Lactobacillus helveticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
pyruvate | - |
Lactobacillus helveticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, almost 2fold stimulation | Lactobacillus helveticus | |
Zn2+ | 1 mM, almost 2fold stimulation | Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | 138000 | gel filtration | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus helveticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2119 | - |
recombinant enzyme | Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH | 3% of the activity with pyruvate | Lactobacillus helveticus | 2-hydroxybutyrate + NAD+ | - |
? | |
phenylpyruvate + NADH | 28% of the activity with pyruvate | Lactobacillus helveticus | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Lactobacillus helveticus | (S)-lactate + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, 90% loss of activity | Lactobacillus helveticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
643 | - |
pyruvate | - |
Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Lactobacillus helveticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | coenzyme | Lactobacillus helveticus |