KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
pyruvate | 20°C, pH 7 | Epidalea calamita | |
0.018 | - |
pyruvate | 15°C, pH 7 | Epidalea calamita | |
0.025 | - |
pyruvate | 15°C, pH 7.4 | Epidalea calamita | |
0.027 | - |
pyruvate | 15°C, pH 7 | Pelophylax perezi | |
0.033 | - |
pyruvate | 20°C, pH 7 | Pelophylax perezi | |
0.039 | - |
pyruvate | 20°C, pH 7.4 | Epidalea calamita | |
0.044 | - |
pyruvate | 15°C, pH 7.4 | Pelophylax perezi | |
0.054 | - |
pyruvate | 20°C, pH 7.4 | Pelophylax perezi | |
0.057 | - |
pyruvate | 15°C, pH 8 | Epidalea calamita | |
0.061 | - |
pyruvate | 30°C, pH 7 | Pelophylax perezi | |
0.061 | - |
pyruvate | 30°C, pH 7 | Epidalea calamita | |
0.078 | - |
pyruvate | 20°C, pH 8 | Epidalea calamita | |
0.086 | - |
pyruvate | 30°C, pH 7.4 | Epidalea calamita | |
0.11 | - |
pyruvate | 15°C, pH 8 | Pelophylax perezi | |
0.117 | - |
pyruvate | 30°C, pH 7.4 | Pelophylax perezi | |
0.145 | - |
pyruvate | 30°C, pH 8 | Epidalea calamita | |
0.171 | - |
pyruvate | 20°C, pH 8 | Pelophylax perezi | |
0.312 | - |
pyruvate | 30°C, pH 7.4 | Pelophylax perezi | |
0.515 | - |
(S)-lactate | 15°C, pH 8 | Epidalea calamita | |
0.517 | - |
(S)-lactate | 20°C, pH 8 | Pelophylax perezi | |
0.537 | - |
(S)-lactate | 20°C, pH 7.4 | Pelophylax perezi | |
0.541 | - |
(S)-lactate | 15°C, pH 8 | Pelophylax perezi | |
0.567 | - |
(S)-lactate | 15°C, pH 7.4 | Epidalea calamita | |
0.616 | - |
(S)-lactate | 20°C, pH 8 | Epidalea calamita | |
0.62 | - |
(S)-lactate | 15°C, pH 7.4 | Pelophylax perezi | |
0.641 | - |
(S)-lactate | 20°C, pH 7.4 | Epidalea calamita | |
0.735 | - |
(S)-lactate | 15°C, pH 7 | Pelophylax perezi | |
0.803 | - |
(S)-lactate | 20°C, pH 7 | Epidalea calamita | |
0.822 | - |
(S)-lactate | 30°C, pH 7 | Pelophylax perezi | |
0.862 | - |
(S)-lactate | 15°C, pH 7 | Epidalea calamita | |
0.909 | - |
(S)-lactate | 30°C, pH 7 | Epidalea calamita | |
0.94 | - |
(S)-lactate | 30°C, pH 7.4 | Epidalea calamita | |
0.966 | - |
(S)-lactate | 20°C, pH 7 | Pelophylax perezi | |
1.016 | - |
(S)-lactate | 30°C, pH 7.4 | Pelophylax perezi | |
1.191 | - |
(S)-lactate | 30°C, pH 8 | Epidalea calamita | |
1.33 | - |
(S)-lactate | 30°C, pH 7 | Pelophylax perezi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lactate + NAD+ | Pelophylax perezi | the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation | pyruvate + NADH | - |
r | |
L-lactate + NAD+ | Epidalea calamita | the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation | pyruvate + NADH | - |
r | |
pyruvate + NADH + H+ | Pelophylax perezi | - |
L-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Epidalea calamita | - |
L-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Epidalea calamita | - |
- |
- |
Pelophylax perezi | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Epidalea calamita | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Pelophylax perezi | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Epidalea calamita | pyruvate + NADH + H+ | - |
r | |
L-lactate + NAD+ | the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation | Pelophylax perezi | pyruvate + NADH | - |
r | |
L-lactate + NAD+ | the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation | Epidalea calamita | pyruvate + NADH | - |
r | |
pyruvate + NADH + H+ | - |
Pelophylax perezi | (S)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Epidalea calamita | (S)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Pelophylax perezi | L-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Epidalea calamita | L-lactate + NAD+ | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Pelophylax perezi | |
NAD+ | coenzyme | Epidalea calamita | |
NADH | coenzyme | Pelophylax perezi | |
NADH | coenzyme | Epidalea calamita |