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Literature summary for 1.1.1.27 extracted from

  • Mendiola, P.; De Costa, J.
    The effects of temperature and pH on the kinetic properties of heart muscle lactate dehydrogenase from anuran amphibians (1991), Comp. Biochem. Physiol. B, 98, 529-534.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
pyruvate 20°C, pH 7 Epidalea calamita
0.018
-
pyruvate 15°C, pH 7 Epidalea calamita
0.025
-
pyruvate 15°C, pH 7.4 Epidalea calamita
0.027
-
pyruvate 15°C, pH 7 Pelophylax perezi
0.033
-
pyruvate 20°C, pH 7 Pelophylax perezi
0.039
-
pyruvate 20°C, pH 7.4 Epidalea calamita
0.044
-
pyruvate 15°C, pH 7.4 Pelophylax perezi
0.054
-
pyruvate 20°C, pH 7.4 Pelophylax perezi
0.057
-
pyruvate 15°C, pH 8 Epidalea calamita
0.061
-
pyruvate 30°C, pH 7 Pelophylax perezi
0.061
-
pyruvate 30°C, pH 7 Epidalea calamita
0.078
-
pyruvate 20°C, pH 8 Epidalea calamita
0.086
-
pyruvate 30°C, pH 7.4 Epidalea calamita
0.11
-
pyruvate 15°C, pH 8 Pelophylax perezi
0.117
-
pyruvate 30°C, pH 7.4 Pelophylax perezi
0.145
-
pyruvate 30°C, pH 8 Epidalea calamita
0.171
-
pyruvate 20°C, pH 8 Pelophylax perezi
0.312
-
pyruvate 30°C, pH 7.4 Pelophylax perezi
0.515
-
(S)-lactate 15°C, pH 8 Epidalea calamita
0.517
-
(S)-lactate 20°C, pH 8 Pelophylax perezi
0.537
-
(S)-lactate 20°C, pH 7.4 Pelophylax perezi
0.541
-
(S)-lactate 15°C, pH 8 Pelophylax perezi
0.567
-
(S)-lactate 15°C, pH 7.4 Epidalea calamita
0.616
-
(S)-lactate 20°C, pH 8 Epidalea calamita
0.62
-
(S)-lactate 15°C, pH 7.4 Pelophylax perezi
0.641
-
(S)-lactate 20°C, pH 7.4 Epidalea calamita
0.735
-
(S)-lactate 15°C, pH 7 Pelophylax perezi
0.803
-
(S)-lactate 20°C, pH 7 Epidalea calamita
0.822
-
(S)-lactate 30°C, pH 7 Pelophylax perezi
0.862
-
(S)-lactate 15°C, pH 7 Epidalea calamita
0.909
-
(S)-lactate 30°C, pH 7 Epidalea calamita
0.94
-
(S)-lactate 30°C, pH 7.4 Epidalea calamita
0.966
-
(S)-lactate 20°C, pH 7 Pelophylax perezi
1.016
-
(S)-lactate 30°C, pH 7.4 Pelophylax perezi
1.191
-
(S)-lactate 30°C, pH 8 Epidalea calamita
1.33
-
(S)-lactate 30°C, pH 7 Pelophylax perezi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + NAD+ Pelophylax perezi the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation pyruvate + NADH
-
r
L-lactate + NAD+ Epidalea calamita the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation pyruvate + NADH
-
r
pyruvate + NADH + H+ Pelophylax perezi
-
L-lactate + NAD+
-
r
pyruvate + NADH + H+ Epidalea calamita
-
L-lactate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Epidalea calamita
-
-
-
Pelophylax perezi
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Epidalea calamita
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Pelophylax perezi pyruvate + NADH + H+
-
r
(S)-lactate + NAD+
-
Epidalea calamita pyruvate + NADH + H+
-
r
L-lactate + NAD+ the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation Pelophylax perezi pyruvate + NADH
-
r
L-lactate + NAD+ the enzyme plays two important roles in heart metabolism, it catalyzes pyruvate reduction, mainly at the beginning of effort, or during hypoxia, and also catalyzes the oxidation of lactate released into the blood by other tissues, such as skeletal muscle, which would be used as substrate fuel by the heart mainly during steady-state exercise or during recuperation Epidalea calamita pyruvate + NADH
-
r
pyruvate + NADH + H+
-
Pelophylax perezi (S)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Epidalea calamita (S)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Pelophylax perezi L-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Epidalea calamita L-lactate + NAD+
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Pelophylax perezi
NAD+ coenzyme Epidalea calamita
NADH coenzyme Pelophylax perezi
NADH coenzyme Epidalea calamita