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Literature summary for 1.1.1.27 extracted from

  • Marchat, L.; Loiseau, P.M.; Petek, F.
    Purification and characterization of lactate dehydrogenase isoenzymes 1 and 2 from Molinema dessetae (Nematoda:Filarioidea) (1996), Parasitol. Res., 82, 672-680.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Molinema dessetae
iodoacetate
-
Molinema dessetae
oxalate noncompetitive with pyruvate, competitive with lactate Molinema dessetae
oxamate competitive with pyruvate, noncompetitive with lactate Molinema dessetae
p-chloromercuribenzoate
-
Molinema dessetae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
NAD+
-
Molinema dessetae
0.25
-
NADH
-
Molinema dessetae
0.34
-
pyruvate
-
Molinema dessetae
2.5
-
(S)-lactate
-
Molinema dessetae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
2 * 58000, isoenzyme LDH1 and LDH2, SDS-PAGE Molinema dessetae
113000
-
gel filtration Molinema dessetae

Organism

Organism UniProt Comment Textmining
Molinema dessetae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two major isoenzymes LDH1 and LDH2 Molinema dessetae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.27
-
-
Molinema dessetae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+ pyruvate reduction is the favored reaction Molinema dessetae pyruvate + NADH + H+
-
r
pyruvate + NADH + H+ pyruvate reduction is the favored reaction Molinema dessetae (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 58000, isoenzyme LDH1 and LDH2, SDS-PAGE Molinema dessetae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 min, 2% loss of activity Molinema dessetae
55
-
stable below Molinema dessetae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
reduction of pyruvate Molinema dessetae
8.6
-
oxidation of lactate Molinema dessetae

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: 85% of maximal activity, pH 8.0: 45% of maximal activity, reduction of pyruvate Molinema dessetae
8 9.6 pH 8.0: 60% of maximal activity, pH 9.6: 45% of maximal activity, oxidation of lactate Molinema dessetae

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Molinema dessetae
NADH coenzyme Molinema dessetae