Cloned (Comment) | Organism |
---|---|
into pQE-30 Xa vector and expressed in Escherichia coli SG13009 cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | the enzyme exhibits characteristic reduced substrate inhibition and enhanced kcat | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the recombinant protein is exclusively associated with inclusion bodies | Plasmodium falciparum | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity yielding 18 mg of protein/litre culture | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
453.8 | - |
- |
Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Plasmodium falciparum |
LDH | - |
Plasmodium falciparum |
Organism | Comment | Expression |
---|---|---|
Plasmodium falciparum | the enzyme is induced at 37°C by 0.5 mM beta-D-thiogalactoside concentration being associated with inclusion bodies. By reducing cell growth temperature to 15°C and sopropyl beta-D-thiogalactoside concentration to 0.25 mM, it is possible to get approximately 82% of expressed protein in soluble form | up |