Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1,6-bisphosphate | best at 4-9 mM | Weizmannia coagulans | |
additional information | the enzyme also shows some activity in the absence of D-fructose-1,6-bisphosphate, with a pH optimum of pH 4.0 | Weizmannia coagulans |
Application | Comment | Organism |
---|---|---|
synthesis | L-nLDH is an efficient catalyst that can be used in the enantioselective reduction of alpha-keto acids to alpha-hydroxy acids | Weizmannia coagulans |
Cloned (Comment) | Organism |
---|---|
gene ldhL, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3) | Weizmannia coagulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Weizmannia coagulans | |
Cu2+ | - |
Weizmannia coagulans | |
Ni2+ | strong inhibition | Weizmannia coagulans | |
Zn2+ | - |
Weizmannia coagulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.91 | - |
pyruvate | recombinant enzyme, pH 6.5, 55°C | Weizmannia coagulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Weizmannia coagulans | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Weizmannia coagulans | |
Ca2+ | activates, most effective divalent metal ion | Weizmannia coagulans | |
Mg2+ | activates | Weizmannia coagulans | |
Mn2+ | activates | Weizmannia coagulans | |
additional information | Ca2+, Ba2+, and Mg2+ ions are as effective as Mn2+ at pH 6.5 in enzyme activation | Weizmannia coagulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Weizmannia coagulans | - |
pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | Weizmannia coagulans NL01 | - |
pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | Weizmannia coagulans | - |
(S)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | Weizmannia coagulans NL01 | - |
(S)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weizmannia coagulans | W8CXQ9 | - |
- |
Weizmannia coagulans NL01 | W8CXQ9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli BL21(DE3) by metal affinity chromatography dialysis, and ultrafiltration | Weizmannia coagulans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
351 | - |
purified recombinant enzyme, pH 11.5, 55°C, lactate oxidation | Weizmannia coagulans |
2323 | - |
purified recombinant enzyme, pH 6.5, 55°C, pyruvate reduction | Weizmannia coagulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Weizmannia coagulans | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Weizmannia coagulans NL01 | pyruvate + NADH + H+ | - |
r | |
2-oxobutyrate + NADH + H+ | low activity | Weizmannia coagulans | 2-hydroxybutyrate + NAD+ | - |
? | |
2-oxobutyrate + NADH + H+ | low activity | Weizmannia coagulans NL01 | 2-hydroxybutyrate + NAD+ | - |
? | |
ethyl pyruvate + NADH + H+ | - |
Weizmannia coagulans | ? + NAD+ | - |
? | |
ethyl pyruvate + NADH + H+ | - |
Weizmannia coagulans NL01 | ? + NAD+ | - |
? | |
methyl pyruvate + NADH + H+ | - |
Weizmannia coagulans | ? + NAD+ | - |
? | |
additional information | L-nLDH shows higher specificity towards pyruvate esters, such as methyl pyruvate and ethyl pyruvate. Very poor activity with 4-methyl-2-oxovalerate | Weizmannia coagulans | ? | - |
? | |
additional information | L-nLDH shows higher specificity towards pyruvate esters, such as methyl pyruvate and ethyl pyruvate. Very poor activity with 4-methyl-2-oxovalerate | Weizmannia coagulans NL01 | ? | - |
? | |
phenylpyruvate + NADH + H+ | low activity | Weizmannia coagulans | L-phenyllactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Weizmannia coagulans | (S)-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | - |
Weizmannia coagulans NL01 | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000-36000, recombinant enzyme, SDS-PAGE | Weizmannia coagulans |
Synonyms | Comment | Organism |
---|---|---|
L-nLDH | - |
Weizmannia coagulans |
ldhL | - |
Weizmannia coagulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme, pyruvate reduction and lactate oxidation | Weizmannia coagulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, half-time for inactivation is about 4 h and no residual activity is determined after 9 h, rapid loss of activity above | Weizmannia coagulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme also shows some activity in the absence of D-fructose-1,6-bisphosphate (FDP), with a pH optimum of pH 4.0. The addition of FDP results in a shift of pH optimum in pyruvate reduction, overview | Weizmannia coagulans |
6.5 | - |
recombinant enzyme, pyruvate reduction | Weizmannia coagulans |
11.5 | - |
recombinant enzyme, lactate oxidation | Weizmannia coagulans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | activity range, inactive at pH 4.0 and pH 10.0 | Weizmannia coagulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Weizmannia coagulans | |
NADH | - |
Weizmannia coagulans |
General Information | Comment | Organism |
---|---|---|
evolution | there are two types of L-nLDHs, non-allosteric L-nLDHs and allosteric L-nLDHs | Weizmannia coagulans |
metabolism | NAD-dependent L-lactate dehydrogenases (L-nLDHs) catalyze the last step of anaerobic glycosis, the reduction of pyruvate to L-lactate, concomitantly oxidizing NADH into NAD+ | Weizmannia coagulans |
additional information | the key catalytic residues Arg109, Asp168, Arg171, and His 195, are conserved in Bacillus coagulans strain NL01 L-nLDH | Weizmannia coagulans |
physiological function | the stereospecific L-LDH is a fructose 1,6-diphosphate-activated NAD-dependent lactate dehydrogenase, L-nLDH | Weizmannia coagulans |