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Literature summary for 1.1.1.27 extracted from

  • Jiang, T.; Xu, Y.; Sun, X.; Zheng, Z.; Ouyang, J.
    Kinetic characterization of recombinant Bacillus coagulans FDP-activated L-lactate dehydrogenase expressed in Escherichia coli and its substrate specificity (2014), Protein Expr. Purif., 95, 219-225.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose-1,6-bisphosphate best at 4-9 mM Weizmannia coagulans
additional information the enzyme also shows some activity in the absence of D-fructose-1,6-bisphosphate, with a pH optimum of pH 4.0 Weizmannia coagulans

Application

Application Comment Organism
synthesis L-nLDH is an efficient catalyst that can be used in the enantioselective reduction of alpha-keto acids to alpha-hydroxy acids Weizmannia coagulans

Cloned(Commentary)

Cloned (Comment) Organism
gene ldhL, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3) Weizmannia coagulans

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Weizmannia coagulans
Cu2+
-
Weizmannia coagulans
Ni2+ strong inhibition Weizmannia coagulans
Zn2+
-
Weizmannia coagulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.91
-
pyruvate recombinant enzyme, pH 6.5, 55°C Weizmannia coagulans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Weizmannia coagulans 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Weizmannia coagulans
Ca2+ activates, most effective divalent metal ion Weizmannia coagulans
Mg2+ activates Weizmannia coagulans
Mn2+ activates Weizmannia coagulans
additional information Ca2+, Ba2+, and Mg2+ ions are as effective as Mn2+ at pH 6.5 in enzyme activation Weizmannia coagulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Weizmannia coagulans
-
pyruvate + NADH + H+
-
r
(S)-lactate + NAD+ Weizmannia coagulans NL01
-
pyruvate + NADH + H+
-
r
pyruvate + NADH + H+ Weizmannia coagulans
-
(S)-lactate + NAD+
-
r
pyruvate + NADH + H+ Weizmannia coagulans NL01
-
(S)-lactate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Weizmannia coagulans W8CXQ9
-
-
Weizmannia coagulans NL01 W8CXQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli BL21(DE3) by metal affinity chromatography dialysis, and ultrafiltration Weizmannia coagulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
351
-
purified recombinant enzyme, pH 11.5, 55°C, lactate oxidation Weizmannia coagulans
2323
-
purified recombinant enzyme, pH 6.5, 55°C, pyruvate reduction Weizmannia coagulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Weizmannia coagulans pyruvate + NADH + H+
-
r
(S)-lactate + NAD+
-
Weizmannia coagulans NL01 pyruvate + NADH + H+
-
r
2-oxobutyrate + NADH + H+ low activity Weizmannia coagulans 2-hydroxybutyrate + NAD+
-
?
2-oxobutyrate + NADH + H+ low activity Weizmannia coagulans NL01 2-hydroxybutyrate + NAD+
-
?
ethyl pyruvate + NADH + H+
-
Weizmannia coagulans ? + NAD+
-
?
ethyl pyruvate + NADH + H+
-
Weizmannia coagulans NL01 ? + NAD+
-
?
methyl pyruvate + NADH + H+
-
Weizmannia coagulans ? + NAD+
-
?
additional information L-nLDH shows higher specificity towards pyruvate esters, such as methyl pyruvate and ethyl pyruvate. Very poor activity with 4-methyl-2-oxovalerate Weizmannia coagulans ?
-
?
additional information L-nLDH shows higher specificity towards pyruvate esters, such as methyl pyruvate and ethyl pyruvate. Very poor activity with 4-methyl-2-oxovalerate Weizmannia coagulans NL01 ?
-
?
phenylpyruvate + NADH + H+ low activity Weizmannia coagulans L-phenyllactate + NAD+
-
?
pyruvate + NADH + H+
-
Weizmannia coagulans (S)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Weizmannia coagulans NL01 (S)-lactate + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 34000-36000, recombinant enzyme, SDS-PAGE Weizmannia coagulans

Synonyms

Synonyms Comment Organism
L-nLDH
-
Weizmannia coagulans
ldhL
-
Weizmannia coagulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
recombinant enzyme, pyruvate reduction and lactate oxidation Weizmannia coagulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant enzyme, half-time for inactivation is about 4 h and no residual activity is determined after 9 h, rapid loss of activity above Weizmannia coagulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
the enzyme also shows some activity in the absence of D-fructose-1,6-bisphosphate (FDP), with a pH optimum of pH 4.0. The addition of FDP results in a shift of pH optimum in pyruvate reduction, overview Weizmannia coagulans
6.5
-
recombinant enzyme, pyruvate reduction Weizmannia coagulans
11.5
-
recombinant enzyme, lactate oxidation Weizmannia coagulans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5 activity range, inactive at pH 4.0 and pH 10.0 Weizmannia coagulans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Weizmannia coagulans
NADH
-
Weizmannia coagulans

General Information

General Information Comment Organism
evolution there are two types of L-nLDHs, non-allosteric L-nLDHs and allosteric L-nLDHs Weizmannia coagulans
metabolism NAD-dependent L-lactate dehydrogenases (L-nLDHs) catalyze the last step of anaerobic glycosis, the reduction of pyruvate to L-lactate, concomitantly oxidizing NADH into NAD+ Weizmannia coagulans
additional information the key catalytic residues Arg109, Asp168, Arg171, and His 195, are conserved in Bacillus coagulans strain NL01 L-nLDH Weizmannia coagulans
physiological function the stereospecific L-LDH is a fructose 1,6-diphosphate-activated NAD-dependent lactate dehydrogenase, L-nLDH Weizmannia coagulans