Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | activates wild-type enzyme | Geobacillus stearothermophilus | |
D-fructose 1,6-bisphosphate | activates wild-type enzyme | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli HB101 using a pEMBL vector. The gene is strongly expressed in the vector used if the orientation of the insert allows the LDH promoter and the vector's lac promoter to direct transcription in the same direction | Geobacillus stearothermophilus |
expression in Escherichia coli HB101 using a pEMBL vector. The gene is strongly expressed in the vector used if the orientation of the insert allows the LDH promoter and the vector's lac promoter to direct transcription in the same direction | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
S100M | a hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH, named S100M, is more thermostable than Bacillus megaterium LDH, less thermostable than Bacillus stearothermophilus LDH and unlike the two wild-type enzymes, it can not be activated by D-fructose 1,6-bisphosphate | Geobacillus stearothermophilus |
S100M | a hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH, named S100M, is more thermostable than Bacillus megaterium LDH, less thermostable than Bacillus stearothermophilus LDH and unlike the two wild-type enzymes, it can not be activated by D-fructose 1,6-bisphosphate | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | slightly inhibits activity of hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Geobacillus stearothermophilus | |
D-fructose 1,6-bisphosphate | slightly inhibits activity of hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | measured without D-fructose 1,6-bisphosphate, wild-type enzyme | Geobacillus stearothermophilus | |
0.08 | - |
NADH | measured with D-fructose 1,6-bisphosphate, wild-type enzyme | Geobacillus stearothermophilus | |
0.15 | - |
NADH | measured without D-fructose 1,6-bisphosphate, wild-type enzyme | Priestia megaterium | |
0.18 | - |
NADH | measured with D-fructose 1,6-bisphosphate, wild-type enzyme | Priestia megaterium | |
0.19 | - |
NADH | measured with D-fructose 1,6-bisphosphate | Geobacillus stearothermophilus | |
0.19 | - |
NADH | measured with D-fructose 1,6-bisphosphate | Priestia megaterium | |
0.19 | - |
NADH | measured without D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Geobacillus stearothermophilus | |
0.19 | - |
NADH | measured without D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Priestia megaterium | |
0.2 | - |
pyruvate | measured with D-fructose 1,6-bisphosphate, wild-type enzyme | Geobacillus stearothermophilus | |
0.8 | - |
pyruvate | measured with D-fructose 1,6-bisphosphate, wild-type enzyme | Priestia megaterium | |
6.8 | - |
pyruvate | measured with D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Geobacillus stearothermophilus | |
6.8 | - |
pyruvate | measured with D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Priestia megaterium | |
7 | - |
pyruvate | measured without D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Geobacillus stearothermophilus | |
7 | - |
pyruvate | measured without D-fructose 1,6-bisphosphate, hybrid enzyme constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331) | Priestia megaterium | |
13 | - |
pyruvate | measured without D-fructose 1,6-bisphosphate, wild-type enzyme | Geobacillus stearothermophilus | |
34 | - |
pyruvate | measured without D-fructose 1,6-bisphosphate, wild-type enzyme | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Priestia megaterium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Geobacillus stearothermophilus | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Priestia megaterium | (S)-lactate + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
a hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH, named S100M, is more thermostable than Bacillus megaterium LDH, less thermostable than Bacillus stearothermophilus LDH and unlike the two wild-type enzymes, it can not be activated by D-fructose 1,6-bisphosphate | Geobacillus stearothermophilus |
additional information | - |
a hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH, named S100M, is more thermostable than Bacillus megaterium LDH, less thermostable than Bacillus stearothermophilus LDH and unlike the two wild-type enzymes, it can not be activated by D-fructose 1,6-bisphosphate | Priestia megaterium |
48 | - |
30 min, stable up to | Priestia megaterium |
58 | - |
30 min, stable up to A hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH is named S100M | Geobacillus stearothermophilus |
58 | - |
30 min, stable up to A hybrid gene is constructed from fragments of the LDH genes from Bacillus stearothermophilus (coding for aa 15-100) and Bacillus megaterium (coding for aa 101-331). The hybrid LDH is named S100M | Priestia megaterium |
74 | - |
30 min, stable up to | Geobacillus stearothermophilus |