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Literature summary for 1.1.1.27 extracted from

  • Crow, V.L.; Pritchard, G.G.
    Fructose 1,6-diphosphate-activated L-lactate dehydrogenase from Streptococcus lactis: kinetic properties and factors affecting activation (1977), J. Bacteriol., 131, 82-91.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
Lactococcus lactis
pyruvate above 20 mM Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
NADH pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate Lactococcus lactis
1.5
-
pyruvate pH 6.9, 90 mM Tris-maleate buffer, pH 6.9, 0.5 mM fructose 1,6-diphosphate Lactococcus lactis
2.4
-
NAD+ in presence of 1 mM fructose 1,6-diphosphate Lactococcus lactis
8
-
NADH without fructose 1,6-diphosphate Lactococcus lactis
100
-
(S)-lactate in presence of 1 mM fructose 1,6-diphosphate Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ no effect Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactococcus lactis the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions ?
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
109
-
-
Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+ inactive towards D-lactate Lactococcus lactis pyruvate + NADH + H+
-
r
additional information the enzyme is unlikely to catalyze lactate oxidation at an appreciable rate under physiological conditions Lactococcus lactis ?
-
?
pyruvate + NADH + H+
-
Lactococcus lactis (S)-lactate + NAD+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
in presence of fructose 1,6-diphosphate Lactococcus lactis
8.2
-
in absence of fructose 1,6-diphosphate Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Lactococcus lactis
NADH coenzyme Lactococcus lactis