Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | not dependent on fructose 1,6-diphosphate | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Fluoropyruvate | - |
Cupriavidus necator | |
Ag+ | - |
Cupriavidus necator | |
Cd2+ | partial | Cupriavidus necator | |
Cu2+ | - |
Cupriavidus necator | |
Hg2+ | - |
Cupriavidus necator | |
NAD+ | - |
Cupriavidus necator | |
Ni2+ | partial | Cupriavidus necator | |
oxalate | - |
Cupriavidus necator | |
oxaloacetate | - |
Cupriavidus necator | |
oxamate | - |
Cupriavidus necator | |
p-chloromercuribenzoate | - |
Cupriavidus necator | |
phosphoenolpyruvate | - |
Cupriavidus necator | |
Zn2+ | - |
Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Cupriavidus necator |
65000 | 67000 | gel filtration | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
824.6 | - |
- |
Cupriavidus necator |
Storage Stability | Organism |
---|---|
-20°C, stable for months | Cupriavidus necator |
stabilized for storage at 4°C by addition of 0.2-1 M potassium chloride or 3.2 M ammonium sulfate | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Cupriavidus necator | pyruvate + NADH + H+ | - |
r | |
2-oxobutyrate + NADH | 107% of the activity with pyruvate | Cupriavidus necator | 2-hydroxybutyrate + NAD+ | - |
? | |
2-oxoglutarate + NADH + H+ | 79.6% of the activity with pyruvate | Cupriavidus necator | 2-hydroxyglutarate + NAD+ | - |
? | |
2-oxopentanoate + NADH | 125% of the activity with pyruvate | Cupriavidus necator | 2-hydroxypentanoate + NAD+ | - |
? | |
3-fluoropyruvate + NADH | 33.6% of the activity with pyruvate | Cupriavidus necator | ? | - |
? | |
3-methyl-2-oxobutanoate + NADH | 28.5% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-3-methylbutanoate + NAD+ | - |
? | |
3-methyl-2-oxopentanoate + NADH | 5.3% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-3-methylpentanoate + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NADH | 39% of the activity with pyruvate | Cupriavidus necator | 2-hydroxy-4-methylpentanoate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Cupriavidus necator | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 45 | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of pyruvate | Cupriavidus necator |
9.8 | - |
oxidation of lactate | Cupriavidus necator |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.3 | 8 | pH 5.3: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, reduction of pyruvate | Cupriavidus necator |
8.5 | 10.7 | pH 8.5: about 60% of maximal activity, pH 10.7: about 70% of maximal activity, oxidation of lactate | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | coenzyme | Cupriavidus necator | |
NADH | coenzyme | Cupriavidus necator |