Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.27 extracted from

  • Betsche, T.; Bosach, K.; Gerhardt, B.
    L-Lactate dehydrogenase from leaves of Capsella bursa-pastoris (L.) Med. I. Identification and partial characterization (1979), Planta, 146, 567-574.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
pyruvate
-
Capsella bursa-pastoris
13
-
(S)-lactate
-
Capsella bursa-pastoris

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Capsella bursa-pastoris 5829
-

Organism

Organism UniProt Comment Textmining
Capsella bursa-pastoris
-
L. Med
-
Cardamine hirsuta
-
-
-
Lactuca sativa
-
-
-
no activity in Rubus idaeus
-
leaf
-
no activity in Taraxacum officinale
-
leaf
-
no activity in Zea mays
-
leaf
-
Raphanus sativus
-
-
-
Spinacia oleracea
-
-
-
Thlaspi arvense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Capsella bursa-pastoris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-
leaf
-
Raphanus sativus
-
leaf
-
Lactuca sativa
-
leaf
-
Capsella bursa-pastoris
-
leaf
-
Thlaspi arvense
-
leaf
-
Cardamine hirsuta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Lactuca sativa pyruvate + NADH + H+
-
r
(S)-lactate + NAD+
-
Capsella bursa-pastoris pyruvate + NADH + H+
-
r
pyruvate + NADH + H+
-
Lactuca sativa (S)-lactate + NAD+
-
r
pyruvate + NADH + H+
-
Capsella bursa-pastoris (S)-lactate + NAD+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reduction of pyruvate Capsella bursa-pastoris
9
-
oxidation of lactate Capsella bursa-pastoris

pH Range

pH Minimum pH Maximum Comment Organism
7.7 9.5 pH 7.7: about 60% of maximal activity, pH 9.5: about 30% of maximal activity, reduction of pyruvate Capsella bursa-pastoris

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Lactuca sativa
NAD+ coenzyme Capsella bursa-pastoris
NADH coenzyme Lactuca sativa
NADH coenzyme Capsella bursa-pastoris