Activating Compound | Comment | Organism | Structure |
---|---|---|---|
fructose 1,6-diphosphate | L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate | Thermoanaerobacter ethanolicus | |
fructose 1,6-diphosphate | at pH 7.5 lactate dehydrogenase activity is absent without fructose 1,6-diphosphate, from pH 5.0 to pH 7.0 lactate dehydrogenase is present without fructose 1,6-diphosphate but is greater in the presence of the activator | Thermoanaerobacter ethanolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Thermoanaerobacter ethanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
NADH | - |
Thermoanaerobacter ethanolicus | |
0.25 | - |
pyruvate | with 1.0 mM fructose 1,6-diphosphate | Thermoanaerobacter ethanolicus | |
2.2 | - |
pyruvate | in absence of fructose 1,6-diphosphate | Thermoanaerobacter ethanolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
4 * 33500, SDS-PAGE | Thermoanaerobacter ethanolicus |
134000 | - |
gel filtration | Thermoanaerobacter ethanolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter ethanolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter ethanolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
589 | - |
- |
Thermoanaerobacter ethanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH | - |
Thermoanaerobacter ethanolicus | 2-hydroxybutyrate + NAD+ | - |
? | |
2-oxoglutarate + NADH + H+ | - |
Thermoanaerobacter ethanolicus | 2-hydroxyglutarate + NAD+ | - |
? | |
pyruvate + NADH + H+ | - |
Thermoanaerobacter ethanolicus | (S)-lactate + NAD+ | - |
? | |
pyruvate + NADPH + H+ | 34% of the activity with NADH | Thermoanaerobacter ethanolicus | (S)-lactate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33500, SDS-PAGE | Thermoanaerobacter ethanolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
900 min, stable | Thermoanaerobacter ethanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermoanaerobacter ethanolicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | about 55% of maximal activity at pH 5.0 and at pH 7.0 | Thermoanaerobacter ethanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | coenzyme | Thermoanaerobacter ethanolicus | |
NADPH | coenzyme | Thermoanaerobacter ethanolicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.375 | - |
NADPH | - |
Thermoanaerobacter ethanolicus |