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Literature summary for 1.1.1.27 extracted from

  • Bryant, F.O.
    Characterization of the fructose 1,6-bisphosphate-activated, L(+)-lactate dehydrogenase from Thermoanaerobacter ethanolicus (1991), J. Enzyme Inhib., 5, 235-248.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Thermoanaerobacter ethanolicus
fructose 1,6-diphosphate at pH 7.5 lactate dehydrogenase activity is absent without fructose 1,6-diphosphate, from pH 5.0 to pH 7.0 lactate dehydrogenase is present without fructose 1,6-diphosphate but is greater in the presence of the activator Thermoanaerobacter ethanolicus

Inhibitors

Inhibitors Comment Organism Structure
NADPH
-
Thermoanaerobacter ethanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
NADH
-
Thermoanaerobacter ethanolicus
0.25
-
pyruvate with 1.0 mM fructose 1,6-diphosphate Thermoanaerobacter ethanolicus
2.2
-
pyruvate in absence of fructose 1,6-diphosphate Thermoanaerobacter ethanolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
4 * 33500, SDS-PAGE Thermoanaerobacter ethanolicus
134000
-
gel filtration Thermoanaerobacter ethanolicus

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter ethanolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermoanaerobacter ethanolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
589
-
-
Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH
-
Thermoanaerobacter ethanolicus 2-hydroxybutyrate + NAD+
-
?
2-oxoglutarate + NADH + H+
-
Thermoanaerobacter ethanolicus 2-hydroxyglutarate + NAD+
-
?
pyruvate + NADH + H+
-
Thermoanaerobacter ethanolicus (S)-lactate + NAD+
-
?
pyruvate + NADPH + H+ 34% of the activity with NADH Thermoanaerobacter ethanolicus (S)-lactate + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33500, SDS-PAGE Thermoanaerobacter ethanolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
900 min, stable Thermoanaerobacter ethanolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Thermoanaerobacter ethanolicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 about 55% of maximal activity at pH 5.0 and at pH 7.0 Thermoanaerobacter ethanolicus

Cofactor

Cofactor Comment Organism Structure
NADH coenzyme Thermoanaerobacter ethanolicus
NADPH coenzyme Thermoanaerobacter ethanolicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.375
-
NADPH
-
Thermoanaerobacter ethanolicus