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Literature summary for 1.1.1.27 extracted from

  • Joensson, M.; Saleihan, Z.; Nes, I.F.; Holo, H.
    Construction and characterization of three lactate dehydrogenase-negative Enterococcus faecalis V583 mutants (2009), Appl. Environ. Microbiol., 75, 4901-4903.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of ldh1 and ldh-2 deletion mutants and of a catalytically inactive double knockout mutant of genes ldh-1 and ldh-2 by gene replacement achieved by double-crossover homologous recombination. The ldh-2 mutant shows hardly any differences in growth and metabolism in comparison to the wild-type. The two mutants lacking ldh-1, on the other hand, produce much less lactate than the wild-type but grew to higher cell densities and have higher final pHs, phenotypes, overview Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + NAD+ Enterococcus faecalis the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
genes ldh-1 and ldh-2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + NAD+
-
Enterococcus faecalis pyruvate + NADH + H+
-
r
L-lactate + NAD+ the wild-type V583 strain converts glucose almost exclusively to L-lactate under anaerobic conditions Enterococcus faecalis pyruvate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
LDH-1
-
Enterococcus faecalis
LDH-2
-
Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Enterococcus faecalis
NADH
-
Enterococcus faecalis

General Information

General Information Comment Organism
additional information the ldh-1 mutants detoxifies excess pyruvate by converting it to acetoin Enterococcus faecalis
physiological function although Ldh-2 contributes to lactate production, Ldh-1 plays the major role in energy metabolism in Enterococcus faecalis Enterococcus faecalis