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Literature summary for 1.1.1.27 extracted from

  • Taguchi, H.; Machida, M.; Matsuzawa, H.; Ohta, T.
    Allosteric and kinetic properties of L-lactate dehydrogenase from Thermus caldophilus GK24, an extremely thermophilic bacterium (1985), Agric. Biol. Chem., 49, 359-365.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
5-phosphoribosyl 1-diphosphate activates Thermus caldophilus
fructose 1,6-diphosphate L-lactate dehydrogenase form which is activated by fructose 1,6-diphosphate Thermus caldophilus
fructose 1,6-diphosphate maximal activation is about: 0.03 mM at pH 6.0, 0.2 mM at pH 7.1, 2 mM at pH 8.0 and 7 mM at pH 9.0 Thermus caldophilus
fructose 2,6-diphosphate activates Thermus caldophilus
glucose 1,6-diphosphate activates Thermus caldophilus
phosphate slight activation of not activated enzyme, inhibition of fructose 1,6-diphosphate activated enzyme Thermus caldophilus

Inhibitors

Inhibitors Comment Organism Structure
fructose 1,6-diphosphate activation at low concentrations, inhibition at high concentrations Thermus caldophilus
phosphate slight activation of non-activated enzyme, inhibition of fructose 1,6-diphosphate activated enzyme Thermus caldophilus
pyruvate strong substrate inhibition at high concentrations, fructose 1,6-diphosphate activated enzyme Thermus caldophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NAD+
-
Thermus caldophilus
0.065
-
NAD+
-
Thermus caldophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + NADH + H+ Thermus caldophilus
-
L-lactate + NAD+
-
?
pyruvate + NADH + H+ Thermus caldophilus GK24
-
L-lactate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
-
GK24
-
Thermus caldophilus GK24
-
GK24
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Thermus caldophilus pyruvate + NADH + H+
-
r
(S)-lactate + NAD+
-
Thermus caldophilus GK24 pyruvate + NADH + H+
-
r
pyruvate + NADH + H+
-
Thermus caldophilus (S)-lactate + NAD+
-
?
pyruvate + NADH + H+
-
Thermus caldophilus GK24 (S)-lactate + NAD+
-
?
pyruvate + NADH + H+
-
Thermus caldophilus L-lactate + NAD+
-
?
pyruvate + NADH + H+
-
Thermus caldophilus GK24 L-lactate + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+ coenzyme Thermus caldophilus
NADH coenzyme Thermus caldophilus