1.1.1.27: L-lactate dehydrogenase

This is an abbreviated version, for detailed information about L-lactate dehydrogenase, go to the full flat file.

Reaction

(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

A4-LDH, AdhE, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, heart LDH, Immunogenic protein p36, L(+)-nLDH, L-(+)-lactate dehydrogenase, L-lactate dehydrogenase B, L-lactic acid dehydrogenase, L-lactic dehydrogenase, L-LDH, lactate dehydrogenase, lactate dehydrogenase A, lactate dehydrogenase B, lactate dehydrogenase NAD-dependent, lactic acid dehydrogenase, lactic dehydrogenase, LctD, LDH, LDH-1, LDH-2, LDH-3, LDH-4, LDH-5, LDH-A, LDH-A4, LDH-m4, LDH1, LDH2, LdhA, LDHB, mLDH, More, muscle LDH, NAD-lactate dehydrogenase, nitric oxideinducible l-lactate dehydrogenase, PfLDH, proteins, specific or class, anoxic stress response, p34, Sa-LDH-1, TeLdhL, Tsac_0416

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.27 L-lactate dehydrogenase

pH Stability

pH Stability on EC 1.1.1.27 - L-lactate dehydrogenase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 7
-
1 h, 25C, pH 3.8: 50% loss of activity, pH 7.0: stable, mutant enzyme with a deletion of 10 N-terminal amino acids
654791
4 - 8
-
rather stable from pH 4.0 to pH 8.0, but rapid decline in activity below pH 4.0 and above pH 8.0
687249
5 - 7.5
-
rapid loss of activity below pH 5.0 and above pH 7.5
286436
5.5 - 7
-
1 h, 25C, pH 5.5: 50% loss of activity, pH 7.0: stable, wilde-type enzyme
654791
5.7 - 7
-
1 h, 25C, pH 5.7: 50% loss of activity, pH 7.0: stable, mutant enzyme with a deletion of 5 N-terminal amino acids
654791
5.8 - 8.2
purified recombinant enzyme, 1-5 h, 70C, completely stable
711181
6.5
-
highest stability at
286435
additional information
incubation of TeLdhL at lower pH results in a rapid inactivation of the enzyme
711181