1.1.1.27: L-lactate dehydrogenase

This is an abbreviated version, for detailed information about L-lactate dehydrogenase, go to the full flat file.

Reaction

(S)-lactate
+
NAD+
=
pyruvate
+
NADH
+
H+

Synonyms

A4-LDH, AdhE, anaerobic lactate dehydrogenase, BbLDH, dehydrogenase, lactate, eLDHA, eLDHB, Epsilon crystallin, epsilon-crystallin, H4-L-lactate dehydrogenase, heart LDH, Immunogenic protein p36, L(+)-nLDH, L-(+)-lactate dehydrogenase, L-lactate dehydrogenase B, L-lactic acid dehydrogenase, L-lactic dehydrogenase, L-LDH, lactate dehydrogenase, lactate dehydrogenase A, lactate dehydrogenase B, lactate dehydrogenase NAD-dependent, lactic acid dehydrogenase, lactic dehydrogenase, LctD, LDH, LDH-1, LDH-2, LDH-3, LDH-4, LDH-5, LDH-A, LDH-A4, LDH-m4, LDH1, LDH2, LdhA, LDHB, mLDH, More, muscle LDH, NAD-lactate dehydrogenase, nitric oxideinducible l-lactate dehydrogenase, PfLDH, proteins, specific or class, anoxic stress response, p34, Sa-LDH-1, TeLdhL, Tsac_0416

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.27 L-lactate dehydrogenase

Cloned

Cloned on EC 1.1.1.27 - L-lactate dehydrogenase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a chimeric bifunctional enzyme composing of galactose dehydrogenase from Pseudomonas fluorescens and lactate dehydrogenase from Bacillus stearothermophilus is successfully constructed and expressed in Escherichia coli
-
development of a recombinant yeast exhibiting efficient lactate production by substituting the coding region of PDC1 on chromosome XII for that of LDH through homologous recombination
expressed in Leuconostoc citreum using a shuttle vector pLeuCM, change of the pyruvate carbon flux in Leuconostoc citreum from D-lactate into L-lactate by heterologous expression of L-lactate dehydrogenase
expressed in Saccharomyces cerevisiae replacing disrupted pyruvate decarboxylase 1 and alcohol dehydrogenase 1
-
expression in Escherichia coli
expression in Escherichia coli as maltose-binding protein fusion protein. CpLDH1 is probably evolved from the same ancestor of CpMalDH1 by a very recent gene duplication that occurs after Cryptosporidium parvum diverges from other apocomplexans
expression in Escherichia coli HB101 using a pEMBL vector. The gene is strongly expressed in the vector used if the orientation of the insert allows the LDH promoter and the vector's lac promoter to direct transcription in the same direction
expression in Escherichia coli strain DH5alpha
expression in Pichia stipidis strain CBS6054, subcloning in Escherichia coli strain DH5alpha
-
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
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expression of wild-type and mutant enzymes in Escherichia coli strain JM109
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gene AA02769 or lctD, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli DH5alpha, expression as His6-tagged protein in Escherichia coli BL21(DE3)
-
gene ldh-1, expression of N-termially His6-tagged enzyme in Escherichia coli BL21
-
gene ldh-a, DNA and amino acid sequence determination and analysis
gene ldhB, from strain NRRL 395 and 99-880, expression of His6-tagged LdhB in Escherichia coli strain BL21(DE3)
-
gene ldhL, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli
genes ldh and ldhB, sequence determination, analysis, and comparison
into expression plasmid pTUB8Myc-His-X-HX under the control of a modified tubulin promoter. Type I strain of Toxoplasma gondii RHDELTAHX transformed with plasmid. Transgenic parasites overexpressing LDH1; into expression plasmid pTUB8Myc-His-X-HX under the control of a modified tubulin promoter. Type I strain of Toxoplasma gondii RHDELTAHX transformed with plasmid. Transgenic parasites overexpressing LDH2
into pET-SUMO and expressed as 6 His Tag in Escherichia coli BL21
into plasmid pET-30a and expressed with a His tag in Escherichia coli BL21/DE3
-
into pQE-30 Xa vector and expressed in Escherichia coli SG13009 cells
-
LDHA, DNA and amino acid sequence determination and analysis, genetic structure, and sequence comparisons, phylogenetic analysis, overview; LDHB, DNA and amino acid sequence determination and analysis, genetic structure, and sequence comparisons, phylogenetic analysis, overview
N-terminal deletion mutants lacking the first 5 and 10 amino acids of the N-terminus are expressed in Escherichia coli
-
the single gene encoding the enzyme is located on chromosome 13
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