3.2.1.14: chitinase
This is an abbreviated version!
For detailed information about chitinase, go to the full flat file.
Word Map on EC 3.2.1.14
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3.2.1.14
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biocontrol
-
colloidal
-
trichoderma
-
chitin-binding
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fusarium
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pathogenesis-related
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serratia
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n-acetylglucosamine
-
marcescens
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chitosan
-
3-like
-
chi3l1
-
solani
-
freezing
-
gaucher
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glcnac
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beta-1,3-glucanase
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phytopathogenic
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cellulase
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rhizoctonia
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oxysporum
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cinerea
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botrytis
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glucanase
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harzianum
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mycelial
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cuticle
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defense-related
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hysteresis
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rhizosphere
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colletotrichum
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chitooligosaccharides
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molt
-
n-acetyl-d-glucosamine
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alternaria
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flounder
-
circulans
-
chitosanase
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entomopathogenic
-
recrystallization
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sclerotiorum
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transglycosylation
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viride
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beauveria
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bassiana
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pythium
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nucleopolyhedrovirus
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anthracnose
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glucocerebrosidase
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gloeosporioides
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environmental protection
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molecular biology
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analysis
-
synthesis
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pharmacology
-
industry
-
degradation
-
medicine
-
biotechnology
-
agriculture
- 3.2.1.14
-
biocontrol
-
colloidal
- trichoderma
-
chitin-binding
- fusarium
-
pathogenesis-related
- serratia
- n-acetylglucosamine
- marcescens
- chitosan
-
3-like
-
chi3l1
- solani
-
freezing
- gaucher
- glcnac
- beta-1,3-glucanase
-
phytopathogenic
- cellulase
- rhizoctonia
- oxysporum
- cinerea
- botrytis
- glucanase
- harzianum
- mycelial
- cuticle
-
defense-related
-
hysteresis
-
rhizosphere
- colletotrichum
- chitooligosaccharides
-
molt
- n-acetyl-d-glucosamine
- alternaria
- flounder
- circulans
- chitosanase
-
entomopathogenic
-
recrystallization
- sclerotiorum
-
transglycosylation
- viride
- beauveria
- bassiana
- pythium
- nucleopolyhedrovirus
-
anthracnose
- glucocerebrosidase
- gloeosporioides
- environmental protection
- molecular biology
- analysis
- synthesis
- pharmacology
- industry
- degradation
- medicine
- biotechnology
- agriculture
Reaction
Synonyms
1,4-beta-poly-N-acetylglucosaminidase, AcD1ChiA, acid mammalian chitinase, acidic chitinase, acidic mammalian chitinase, active phase-associated protein I, active phase-associated protein II, AD2PF-ChiA, AgChi, alpha-chitinase, AMCase, antifreeze protein, APAP1, APAP2, APAPI, APAPII, AtChiC, B220, bacterial-type chitinase, Bbchit1, BChi14, beta-1,4-poly-N-acetyl glucosamidinase, BjCHI1, BJL200-ChiC1, CF-AG, CF-antigen, CGCHI3, CH5B, ChBDChiC, CHI, Chi-1, CHI-26, Chi-A, Chi-h, Chi1, Chi18A, Chi18B, Chi18C, Chi19, CHI2, Chi2 enzyme, Chi21, Chi22, Chi3, Chi31, Chi35, Chi40, Chi44, Chi46, Chi54, CHI60, Chi67, Chi70, Chi72, Chi78, ChiA, ChiA Nima, ChiA protein, ChiA-HD73, ChiA-Hh59, ChiA-Mt45, ChiA1, ChiA74, ChiB, ChiC, ChiCW, ChiD, ChiE, ChiEN1, ChiF, ChiF1, ChiG, ChiNCTU2, Chit, CHIT 1A, CHIT 1B, CHIT1, Chit1b, Chit33, CHIT42, Chit62, ChitA, ChitIII-1, ChitIII-2, chitinase, chitinase 1, chitinase 18C, chitinase 2, chitinase 60, chitinase 92, chitinase A, chitinase A1, chitinase B, chitinase C, chitinase C1, chitinase Chi255, chitinase F1, chitinase G, chitinase I, chitinase II, chitinase III, chitinase-A, chitinases A, chitinolytic enzyme, chitodextrinase-N-, chitotriosidase, ChiW, CHT1, Cht4, CHT7, ChtII, ChtIII, CiX1, class I chitinase, class II chitinase, class III chitinase, class IV chitinase, class V chitinase, Complement-fixation antigen, CpCHI, CrChi1, CTS1, ECH, ECH1, Ech42, ech46, ELC, endo-chitinase, endochitinase, endochitinase 1, endochitinase-1, endochitinase-2, exochitinase, GAC1, GAC2, GH18 chitinase, group I chitinase, group II chitinase, group III chitinase, hevamine, HoChiA, HoChiB, HoChiC, HschiA1, Is-chiB, LlChi18A, LpChiA, MACase, MF1 antigen, MmChi60, More, MsChi386, MsChi535, NaCHIT1, NaCHIT3, NtChitIV, NtChiV, OfChtI, OsChia1b, OsChia1c, OsChia1cDELTAChBD, OsChia1d, OsChia2a, OsChia2b, OsChia4a, OsChib1a, PF1233, PjChi-1, plant class III chitinase, PLC-A, PLC-B, PLChiA, poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase, poly-beta-glucosaminidase, PrChi-A, Ptchi19p, Ra-ChiC, RCB4, RHBC, sAMC, SCChi-1, SI-CLP, SjChi, SsChi50, stabilin-1 interacting chitinase-like protein, STK_09390, TBC-1, Tfu_0580, Tk-ChiA, TKU028 chitinase, TmChi, TSA1902, UDA, V-CHIA, VpChiA, WIN6, YlCts1p, Ym1
ECTree
3.2.1.14 chitinaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.14 - chitinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(GlcNAc)6 + H2O
(GlcNAc)2 + (GlcNAc)3 + (GlcNAc)4
the enzyme prefers the beta-anomer of chito-oligosaccharides as substrate, cleavage of beta-glycosidic linkages from the non-reducing end primarily by disaccharide units
product analysis
-
r
2-acetamido-2-deoxy-4-O-(2-acetamido-2-deoxy-4-O-methyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-acetamido-4-O-cyclohexylmethyl-2-deoxy-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-deoxy-2-acetamido-4-O-isopropyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl 2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranoside + H2O
?
-
-
-
-
?
4-methylumbelliferyl 2-acetamido-4-O-(2-acetamido-2,4-dideoxy-beta-D-xylo-hexopyranosyl)-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferol + 2-acetamido-4-O-(2-acetamido-2,4-dideoxy-beta-D-xylo-hexopyranosyl)-2-deoxy-beta-D-glucopyranoside
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferone + beta-D-N,N',N''-triacetylchitotriose
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-D-N,N'-diacetylchitobioside
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O
4-methylumbelliferol + beta-N-acetyl-D-glucosamine
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
4-methylumbelliferyl N,N',N''-beta-D-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-beta-D-triacetylchitotriose
substrate is prefered over shorter GlcNAc-substrates
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
4-methylumbelliferyl N,N'-diacetylchitotriose + H2O
?
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
4-methylumbelliferyl-D-N,N'-diacetylchito-bioside + H2O
4-methylumbelliferone + D-N,N'-diacetylchito-bioside
-
-
-
-
?
4-nitrophenyl beta-1,4-N,N'-diacetyl-chitobiose + H2O
4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose
-
-
-
?
4-nitrophenyl beta-D- N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide
-
535% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
-
-
-
-
?
4-nitrophenyl beta-D-GlcNAc + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
0.8% of the activity with 4-nitrophenyl-(beta-D-GlcNAc)3
-
-
?
4-nitrophenyl beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetyl-beta-D-glucosamine
-
700% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide + beta-D-N,N'-diacetylchitobioside + N-acetylglucosamine
-
-
-
?
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + 3 H2O
4-nitrophenol + 2 N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenol + beta-N,N',N'',N'''-tetraacetylchitotetraose
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-N,N',N''-triacetylchitotriose
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + beta-N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
4-nitrophenyl N,N',N''-beta-D-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-beta-D-triacetylchitotriose + N,N'-diacetyl-beta-D-chitobiose + 4-nitrophenyl N-acetyl-beta-D-glucosaminide
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-nitrophenyl N-acetyl-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetylchitobioside + H2O
4-nitrophenol + N-acetylchitobiose
-
-
-
-
?
4-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-nitrophenol + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
-
?
alpha-chitin + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
alpha-type chitosan + H2O
?
-
98% deacetylated, 18% of the activity with colloidal chitin
-
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
beta-D-N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
-
-
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + 2 H2O
2 beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + N-acetyl-beta-D-glucosamine
-
-
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
86% activity compared to colloidal chitin
-
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
beta-D-N,N'-diacetylchitobiose + ?
-
-
-
?
beta-D-N,N',N''-triacetylchitotriose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
beta-type chitin + H2O
?
-
best substrate, 148% of the activity with colloidal chitin
-
-
?
beta-type chitosan + H2O
?
-
98% deacetylated, 26% of the activity with colloidal chitin
-
-
?
chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine
chitin + H2O
N,N-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
?
chitin flakes + H2O
N-acetyl-beta-D-glucosamine + ?
-
10.8% activity compared to colloidal chitin
-
-
?
chitin oligosaccharides + H2O
N-acetyl glucosamine
glycol chitin assay method, (GlcNAc)1-6
-
-
?
chitin powder + H2O
N-acetyl-beta-D-glucosamine + ?
-
32.5% activity compared to colloidal chitin
-
-
?
chitohexaose + H2O
2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose
reaction via trimer formation
-
-
?
chitohexaose + H2O
chitotetraose + chitobiose
-
the enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)6
-
-
?
chitopentaose + H2O
N-acetylglucosamine + 2 N,N'-diacetylchitobiose
reaction via trimer formation
-
-
?
chitosan + H2O
glucosamine + chitobiose + chitooligomers with a chain length longer than 6
-
-
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + ?
-
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
colloidal chitin + H2O
chitobiose + D-glucosamine
from crab shell
-
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetyl D-glucosamine
-
-
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + chitobiose + chitotriose
-
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + tetra-N-acetyl-beta-D-glucosamine
100% activity
-
-
?
crab shell chitin + H2O
?
the enzyme cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly
-
-
?
crystalline alpha-chitin + H2O
GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)-GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta
-
-
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNACbeta(1-4)GlcNAcbeta
-
-
some (GlcNAc)3 is produced through transglycosylation reaction
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAc
-
i.e. (GlcNAc)6
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
i.e. (GlcNAc)6
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
-
-
product distribution is (GlcNAc)3 >(GlcNAc)4 > (GlcNAc)2 for the wild type enzyme. Some (GlcNAc)5 is produced, without formation of GlcNAc, suggesting that it is not directly produced from the initial substrate (GlcNAc)6 but produced through transglycosylation reaction
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
glycol chitin + H2O
N,N'-diacetylchitobiose + GlcNAc
-
exochitinase activity that yields p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose, and then when the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is accumulated, the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is further cleaved into N,N'-diacetylchitobiose and GlcNAc
-
-
?
glycol chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
-
-
-
?
hexa-N-acetyl-chitohexaose + H2O
2 tri-N-acetyl-chitotriose
highest activity
-
-
?
N,N',N'''-triacetyl-beta-D-chitotriose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
-
a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant. BcChi-A catalyzes an anomer inversion reaction
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
the enzyme hydrolyzes the third and fourth glycosidic linkages from the non-reducing end of N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose
-
-
?
N,N',N'',N''', N''''-pentaacetylchitopentaose + H2O
5 N-acetyl-D-glucosamine
-
-
-
?
N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose + H2O
?
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N''',N''''-pentaacetylchitopentaose
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
-
wild-type enzyme: the alpha/beta ratios of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose are 26.8 and 2.95, respectively
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity
-
?
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
N,N',N'',N''',N'''',N'''''-pentacetyl-beta-D-chitopentaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
(GlcNAc)2 + (GlcNAc)3
-
-
a mixture of the alpha- and beta-anomers, alpha-anomers are more dominant
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
2 N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
4 N-acetyl-beta-D-glucosamine
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N-acetylglucosamine + N,N'-diacetylchitobiose
reaction via trimer formation
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + ?
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose
-
-
transglycosilation activity
-
?
N,N',N'',N'''-tetracetyl-beta-D-chitotetraose + H2O
N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N''-triacetyl-beta-D-chitotriose + H2O
N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
N,N',N''-triacetylchitotriose + 2 H2O
3 beta-N-acetyl-D-glucosamine
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetylglucosamine
-
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + GlcNAc
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-cellobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O
D-galactopyranose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
D-glucopyranose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-lactopyranoside + H2O
D-lactopyranose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-maltoside + H2O
D-maltose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O
D-mannopyranose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
N,N',N''-triacetylchitotriose + p-nitrophenol
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc
-
-
-
?
p-nitrophenyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
p-nitrophenyl-N,N',N''-triacetylchitotriose + H2O
p-nitrophenol + N,N'-diacetylchitobiose + GlcNAc
-
-
-
?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
partially deacetylated chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
tri-N-acetyl-chitotriose + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
substrate for isoform Chi78
-
-
?
3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O
?
-
-
-
-
?
3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
-
product analysis
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
product analysis
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
-
-
-
-
?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferone + chitobiose
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
-
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
-
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-(GlcNAc) + H2O
4-methylumbelliferone + N-acetyl-D-glucosamine
-
-
-
?
4-methylumbelliferyl-(GlcNAc) + H2O
4-methylumbelliferone + N-acetyl-D-glucosamine
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate
-
-
?
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
Niallia circulans No. 4.1
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
substrate concentration of 500 microM in citrate buffer
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
-
chit37 and chit42
-
-
?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose
-
-
?
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
-
-
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotriose and 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 1 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
hydrolysing activity against 4-methylumbelliferyl-N,N'-diacetylchitobiose is three times greater than of 4-methylumbelliferyl-N,N',N''-triacetylchitotrioseand 4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose, chitinase 2 is an endochitinases that liberates N,N'-diacetylchitobiose as a main product
-
-
?
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
-
activity with 4-methylumbelliferyl-N,N'-diacetylchitobiose is higher than activity with 4-methylumbelliferyl-N,N',N''-triacetylchitotriose
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
-
-
-
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
250% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
198% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
highest activity
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
highest activity
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
185% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
-
150% activity compared to colloidal chitin
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Salmonella enterica typhimurium
-
-
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
highest activity
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
Thermobifida fusca ATCC BAA-629
highest activity
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
-
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
-
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
?
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
-
-
-
?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
?
essential role of the two carboxylic acid residues E172 and D170 in the catalytic mechanism of Chit42
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
?
-
-
-
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
synthetic substrate
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
best substrate
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
best substrate
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
p-nitrophenol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
p-nitrophenol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl tri-N-acetylchitotrioside + H2O
4-nitrophenol + tri-N-acetylchitotriose
-
-
-
-
?
4-nitrophenyl tri-N-acetylchitotrioside + H2O
4-nitrophenol + tri-N-acetylchitotriose
-
-
-
-
?
alpha-chitin + H2O
?
-
strain LMG 11386 shows low enzyme activity
-
-
?
alpha-chitin + H2O
?
2.1% activity compared to colloidal chitin
-
-
?
alpha-crystal chitin + H2O
di-N-acetyl-chitobiose
-
-
-
?
alpha-type chitin + H2O
?
-
99% of the activity with colloidal chitin
-
-
?
beta-1,4-N-acetylglucosamine polymer + H2O
?
-
linear polymer, endo- and exo-hydrolytic activity
-
-
?
beta-1,4-N-acetylglucosamine polymer + H2O
?
-
linear polymer, endo- and exo-hydrolytic activity
-
-
?
beta-chitin + H2O
?
4.3% activity compared to colloidal chitin
-
-
?
beta-chitin + H2O
?
substrate 3 micrometer beta-chitin
-
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
beta-D-N,N',N'',N''', N''''-pentacetylchitopentaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose
-
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
-
-
?
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetracetylchitotetraose
-
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
-
-
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
best substrate
-
-
?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
Paenibacillus barengoltzii CAU904
best substrate
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 beta-D-N,N'-diacetylchitobiose
-
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 beta-D-N,N'-diacetylchitobiose
Paenibacillus barengoltzii CAU904
-
-
-
?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 beta-D-N,N'-diacetylchitobiose
-
-
-
?
carboxymethyl cellulose + H2O
?
-
1.9% of the activity with colloidal chitin
-
-
?
carboxymethyl cellulose + H2O
?
3.5% activity compared to colloidal chitin
-
-
?
carboxymethyl cellulose + H2O
?
Paenibacillus barengoltzii CAU904
3.5% activity compared to colloidal chitin
-
-
?
carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O
?
Salmonella enterica typhimurium SL1344
-
-
-
-
?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
?
-
-
-
-
?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
?
-
-
-
?
carboxymethylated-chitin-remazol brilliant violet + H2O
?
-
isoenzymes I and II
-
-
?
cell wall + H2O
chitin oligosaccharides
-
from Rhizopus delemar
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
PLC-B hydrolyses Micrococcus lysodeikticus cell wall
-
-
?
cell wall + H2O
chitin oligosaccharides
-
from yeast
N-acetetylglucosamine and diacetylchitobiose
?
cell wall + H2O
chitin oligosaccharides
-
fungal, Phytium and Rhizoctonia solani
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
fungal, Phytium and Rhizoctonia solani
N-acetylglucosamine only
?
cell wall + H2O
chitin oligosaccharides
-
chit42, cell wall from Botrytis cinerea
-
-
?
chitin + H2O
?
-
high activity, the enzyme of strain 16-3 is active with colloidal chitin and mycelia of Trichoderma virens, Mucor hiemalis strain Bu 10, and Trichoderma harzianum strain Mn 75, overview
-
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
-
?
chitin + H2O
?
-
swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin
-
-
?
chitin + H2O
?
-
swollen chitin, partially deacetylated chitin and lower activity towards colloidal chitin
-
-
?
chitin + H2O
?
-
colloidal , regenerated, or partially N-acetylated chitin substrates
-
-
?
chitin + H2O
?
-
optimal substrate concentration is 0.6 mg and 0.4 mg enzyme per reaction mixture
-
-
?
chitin + H2O
?
-
colloidal , regenerated, or partially N-acetylated chitin substrates
-
-
?
chitin + H2O
?
-
the enzyme plays a role in pathogen defense and serves as biocontrol agent against the adult Japanese pine sawyer, Monochamus alternatus, oral ingestion of the enzyme by the insect causes high mortality, the enzyme degrades peritrophic membrane chitin in the beetle, but does not affect the midgut epithelium
-
-
?
chitin + H2O
?
-
colloidal and patially deacetylated chitin PC-100
-
-
?
chitin + H2O
?
-
colloidal and patially deacetylated chitin PC-100
-
-
?
chitin + H2O
?
cell wall chitin of Septoria tritici, Chi46 enzyme can also efficiently degrade cell walls of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici, and Phytophthora sojae
-
-
?
chitin + H2O
?
active toward soluble and insoluble chitin preparations
-
-
?
chitin + H2O
?
active toward soluble and insoluble chitin preparations
-
-
?
chitin + H2O
?
-
substrate of CHITI1 and AMCase, the polysaccharide is contained in many human parasites
-
-
?
chitin + H2O
?
a flexible loop controlling the enzymatic activity and specificity in the glycosyl hydrolase family 19 endochitinase from barley seeds
-
-
?
chitin + H2O
?
-
the enzymes of both strains are active with colloidal chitin and mycelia of Trichoderma virens, Mucor hiemalis strain Bu 10, and Trichoderma harzianum strain Mn 75, overview
-
-
?
chitin + H2O
?
-
isolated from crab shell, 18% of the activity with colloidal chitin for isoform Chi-56, 27% for isoform Chi-64
-
-
?
chitin + H2O
?
chitinases hydrolyse the beta-1,4-linkages in chitin
-
-
?
chitin + H2O
?
-
chitinase catalyzes the hydrolysis of a beta-1,4 glycosidic bond in chitin which is a linear polysaccharide of N-acetyl-beta-D-glucosamine
-
-
?
chitin + H2O
?
-
the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C
-
-
?
chitin + H2O
?
Niallia circulans WL-12
-
the chitin-binding domain CBD exhibits high specificity for and stable binding to chitin at pH 5.0-8.0 and 15-37°C
-
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
-
?
chitin + H2O
?
-
fungal cell-wall chitin is the preferred substrate, the enzyme is also active with CM-, DDD-, colloidal chitin, and chitin from cattle fish, substrate specificity, overview
-
-
?
chitin + H2O
?
chitin from fungal cell wall, the chitinase exhibits different antifungal activities against the four fungi, which is directly correlated to the surface microstructure and the proportion of chitin in the fungal cell wall, antifungal mechanism of action of chitinase against four plant pathogenic fungi, i.e. Rhizopus stolonifer, Botrytis squamosa, Pythium aphanidermatum, and Aspergillus niger, overview
-
-
?
chitin + H2O
?
-
chitinase is a glycoside hydrolase that catalyzes the degradation of chitin
-
-
?
chitin + H2O
?
the enzyme acts from non-reducing ends. Substrate binding structure, overview. The reducing sugar at subsite -1 is in an energetically unfavoured boat conformation, a state that possibly exists just before the completion of catalysis. A hydrophobic plane composed of four surface-exposed aromatic residues is adjacent to the entrance to the substrate-binding cleft
-
-
?
chitin + H2O
?
-
the enzyme shows a high affinity of the enzyme for chitin
-
-
?
chitin + H2O
?
-
the enzyme shows a high affinity of the enzyme for chitin
-
-
?
chitin + H2O
?
-
recombinant enzyme hydrolyzes colloidal and crystalline chitins
-
-
?
chitin + H2O
?
-
the chitinase is a hyperthermophilic glycosidase that effectively hydrolyzes both alpha and beta crystalline chitins, carbohydrate recognition by the chitin-binding domain and substrate binding structure, overview
-
-
?
chitin + H2O
?
-
the surface resiudes Trp274, Trp308 and Trp326 flanked by acidic residues Glu279 and Asp281 are involved in chitin binding
-
-
?
chitin + H2O
?
-
crystalline beta-chitin, molecular weight analysis of oligomer products, overview
-
-
?
chitin + H2O
?
-
crystalline beta-chitin, molecular weight analysis of oligomer products, overview
-
-
?
chitin + H2O
?
-
coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin
-
-
?
chitin + H2O
?
-
the enzyme is capable of degrading a colored colloidal chitin substrate (carboxymethyl-chitin-remazol-brilliant violet) and a small, presumably amorphous, subfraction of alpha-chitin and beta-chitin, but is not capable of degrading crystalline chitin completely
-
-
?
chitin + H2O
?
-
the enzyme is capable of degrading a colored colloidal chitin substrate (carboxymethyl-chitin-remazol-brilliant violet) and a small, presumably amorphous, subfraction of alpha-chitin and beta-chitin, but is not capable of degrading crystalline chitin completely
-
-
?
chitin + H2O
?
-
coloidal chitin and glycol chitin prepared from commercial powdered chitin, prawn shell chitin, crystalline beta-chitin, soluble chitin
-
-
?
chitin + H2O
?
-
colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin
-
-
?
chitin + H2O
?
-
colloidal chitin: 30% of the activity with ethylene glycol chitin, powdered chitin: 4.5% of the activity with ethylene glycol chitin
-
-
?
chitin + H2O
?
-
low activity, the enzyme is equally active with colloidal chitin and mycelia of Trichoderma virens, overview
-
-
?
chitin + H2O
?
-
low activity, the enzyme prefers mycelia from Trichoderma harzianum strain Mn 75 as substrate, followed by colloidal chitin, overview
-
-
?
chitin + H2O
?
-
low activity with crystallin chitin, high activity with colloidal chitin
-
-
?
chitin + H2O
chitotriose + ?
-
colloidal and swollen chitin
a major product from colloidal chitin
-
?
chitin + H2O
chitotriose + ?
-
colloidal and swollen chitin
a major product from colloidal chitin
-
?
chitin + H2O
GlcNAc2 + ?
-
colloidal and ethylene glycol chitin, exo-type cleavage mode
main product from insoluble colloidal chitin
-
?
chitin + H2O
GlcNAc2 + ?
-
colloidal and ethylene glycol chitin, exo-type cleavage mode
main product from insoluble colloidal chitin
-
?
chitin + H2O
N,N'-diacetylchitobiose + ?
-
colloidal
predominant product
-
?
chitin + H2O
N,N'-diacetylchitobiose + ?
-
colloidal
predominant product
-
?
chitin + H2O
N,N'-diacetylchitobiose + ?
insoluble, powdered chitin and colloidal chitin, and chitin nanofiber
final product
-
?
chitin + H2O
N,N'-diacetylchitobiose + ?
chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine
-
-
?
chitin + H2O
N,N'-diacetylchitobiose + ?
chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine
-
-
?
chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine
-
isoform ChiA is the most effective enzyme toward insoluble chitin, isoform ChiC1 is the most effective enzyme toward soluble chitin, ChiA, ChiB and ChiC1 hydrolyze water-soluble chitin very efficiently but powdered chitin is a very poor substrate
(GlcNAc)2 is almost exclusively generated from ChiA, ChiB and ChiC1, ChiA produced as minor products GlcNAc and (GlcNAc)3, ChiB produced as minor product only (GlcNAc)3, ChiC1 produced as minor product only GlcNAc
-
?
chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine
-
isoform ChiA is the most effective enzyme toward insoluble chitin, isoform ChiC1 is the most effective enzyme toward soluble chitin, ChiA, ChiB and ChiC1 hydrolyze water-soluble chitin very efficiently but powdered chitin is a very poor substrate
(GlcNAc)2 is almost exclusively generated from ChiA, ChiB and ChiC1, ChiA produced as minor products GlcNAc and (GlcNAc)3, ChiB produced as minor product only (GlcNAc)3, ChiC1 produced as minor product only GlcNAc
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from shrimp shell, deproteinized
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chitobiose, chitotriose and N-acetylglucosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chitobiose, chitotriose and N-acetylglucosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
nascent more effectively than preformed chitin
diacetylchitobiose only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chitobiose and chitotriose main products
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chitobiose and chitotriose main products
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
crab chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
Calvatia cyanthiformis
-
from Limulus polyphemus, deproteinized
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
basic chitinase produces chitobiose and chitotriose, acidic chitinase produces chitobiose and chitotetraose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
chitobiose main product, chitotetraose and chitotriose also
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from ground crab shell
chitobiose, chitotriose and N-acetylglucosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from Limulus polyphemus, deproteinized
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen chitin
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
oligochitosaccharides, chitobiose being predominant
ir
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
nascent more effectively than preformed chitin
N-N'-diacetylchitobiose main product, also chitotriose and chitotetraose, with preformed chitin as substrate also N-acetylglucosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
purified and colloidal chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
isoenzymes A and B chitotetraose and chitotriose main products, also chitobiose and higher chitooligomers, isoenzymes C1-C4 main product chitobiose, also N-acetylglucosamine and some chitotriose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from ground crab shell
chitobiose and chitotriose, some chitotetraose also
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin: trace amounts of product, regenerated chitin: slowly, powdered chitin: rapidly
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[3H]chitin, isoenzymes I and II show lysozyme activity
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
nascent more effectively than preformed chitin
chitobiose and a small amount of chitotriose only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
swollen with hydrochloric acid
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from ground crab shell
with high ratio of chitinase to chitin N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
with high ratio of chitinase to chitin N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
diacetylchitobiose only, some trisaccharides in early stages
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and regenerated chitin, chitin from suqid and powdered prawn shell
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and regenerated chitin, chitin from suqid and powdered prawn shell
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
N-acetylhexosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and powdered chitin
mainly chitobiose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
-
N-acetylhexosamine
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and powdered chitin
mainly chitobiose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and glycol chitin, powdered chitin very slowly
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from ground crab shell
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
chit37 and chit42 main product chitobiose, some N-acetylglucosamine and chitotriose also, chit33 main product chitotetraose, chitobiose, chitotetraose, n-acetylglucosamine also present
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
[acetyl-3H]chitin
chitobiose, biotriose and biotetraose from nascent chitin, higher oligosaccharides from preformed chitin
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
from ground crab shell
N-acetylglucosamine only
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
also chitotriose, chitotetraose, chitopentaose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal chitin
also chitotriose, chitotetraose, chitopentaose
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
-
colloidal and powdered chitin
chitobiose, chitotriose and N-acetylglucosamine
?
chitin + H2O
N-acetylglucosamine + N-acetylchitobiose
-
processive chitinase with endo-type activity
-
-
?
chitin + H2O
N-acetylglucosamine + N-acetylchitobiose
Niallia circulans No. 4.1
-
processive chitinase with endo-type activity
-
-
?
chitin azure + H2O
?
-
98% activity compared to colloidal chitin
-
-
?
chitin azure + H2O
?
Hydrogenophilus hirschii KB-DZ44
-
98% activity compared to colloidal chitin
-
-
?
chitin azure + H2O
?
-
97% activity compared to colloidal chitin
-
-
?
chitin azure + H2O
?
-
97% activity compared to colloidal chitin
-
-
?
chitohexaose + H2O
chitobiose + ?
-
-
enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose
-
?
chitohexaose + H2O
chitobiose + ?
-
-
enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose
-
?
chitohexaose + H2O
chitobiose + chitotetraose + chitotriose
-
-
-
?
chitohexaose + H2O
chitobiose + chitotriose
-
-
chitobiose and chitotetraose
?
chitohexaose + H2O
chitobiose + chitotriose
-
GBC-a
and chitotetraose
?
chitohexaose + H2O
chitobiose + chitotriose
-
PLC-A and PLC-B
PLC-B N-acetylglucosamine, chitobiose and chitotetraose only
?
chitohexaose + H2O
chitobiose + chitotriose
-
N-acetylglucosamine and chitobiose, both isoenzymes
-
-
?
chitohexaose + H2O
chitobiose + chitotriose
-
N-acetylglucosamine and chitobiose, both isoenzymes
-
-
?
chitooligosaccharide + H2O
?
-
76% activity compared to colloidal chitin
-
-
?
chitooligosaccharide + H2O
?
-
77% activity compared to colloidal chitin
-
-
?
chitooligosaccharides + H2O
?
-
degradation of a 64% acetylated chitosan yields a continuum of oligomers, showing that ChiG operates according to a nonprocessive, endo mode of action
-
-
?
chitooligosaccharides + H2O
?
-
degradation of a 64% acetylated chitosan yields a continuum of oligomers, showing that ChiG operates according to a nonprocessive, endo mode of action
-
-
?
chitooligosaccharides + H2O
?
-
higher than trisaccharides, also reduced chitooligosaccharides more than tetrasaccharides
-
-
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
chitobiose only
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
chitobiose only
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
chitobiose, chitotetraose and trace amounts of chitotriose
?
chitopentaose + H2O
chitobiose + chitotriose
-
PLC-A, PLC-B
PLC-B chitotetraose and N-acetylglucosamine only
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
chitobiose and N-acetylglucosamine, both isoenzymes
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
chitobiose and N-acetylglucosamine, both isoenzymes
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also
?
chitopentaose + H2O
chitobiose + chitotriose
-
-
main product chitobiose, small amounts of chitotriose and N-acetylglucosamine also
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
?
-
chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview
-
-
?
chitosan + H2O
?
-
chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview
-
-
?
chitosan + H2O
?
-
60-95% deacetylated, 24-36% of the activity with colloidal chitin
-
-
?
chitosan + H2O
?
-
60-95% deacetylated, 24-36% of the activity with colloidal chitin
-
-
?
chitosan + H2O
?
-
chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview
-
-
?
chitosan + H2O
?
-
chitosan-succinate is the preferred substrate, the enzyme is also active with CM-chitosan, colloidal chitosan, chtiosan-asparatae and chitosan-glutamate, substrate specificity, overview
-
-
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
?
-
soluble chitin-derivative chitosan, molecular weight analysis of oligomer products, overview
-
-
?
chitosan + H2O
?
-
soluble chitin-derivative chitosan, molecular weight analysis of oligomer products, overview
-
-
?
chitosan + H2O
?
-
75% deacetylated chitosan, 83.8% activity compared to colloidal chitin
-
-
?
chitosan + H2O
?
-
75% deacetylated chitosan, 83.8% activity compared to colloidal chitin
-
-
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
?
-
chitosan with different degrees of deacetylation
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
different percentages of acetylation
-
-
?
chitosan + H2O
chitosan oligosaccharides
11.8% of the activity with colloidal chitin
-
-
?
chitosan + H2O
chitosan oligosaccharides
Thermochaetoides thermophila
17.4% of the activity with colloidal chitin
-
-
?
chitosan + H2O
chitosan oligosaccharides
-
reacetylated
from diacetylchitobiose to tetraacetylchitotetraose
?
chitosan 7B + H2O
?
-
the activity increases with increasing acetylation of the substrate
-
-
?
chitosan 7B + H2O
?
-
the activity increases with increasing acetylation of the substrate
-
-
?
chitotetraose + H2O
2 chitobiose
-
-
enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose
-
?
chitotetraose + H2O
2 chitobiose
-
-
enzyme deletion mutant rapidly hydrolyses chitohexaose and chitotetraose to chitobiose compared to chimera which hydrolyses chitohexaose and chitotetraose to chitotriose and chitobiose
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
chitobiose and chitotetraose
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
scarcely hydrolyzed
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
N-acetylglucosamine only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
PLC-A
chitobiose only
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
-
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
chitobiose, both isoenzymes
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
chitobiose, both isoenzymes
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
-
tritiated
only traces of chitotriose and N-acetylglucosamine
?
chitotriose + H2O
?
-
85% activity compared to colloidal chitin
-
-
?
CM-chitin-RBV + H2O
?
-
i.e carboxymethyl-Chitin-Remazol-Brilliant-Violet, a colloidal substrate
-
-
?
CM-chitin-RBV + H2O
?
BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV
-
-
?
CM-chitin-RBV + H2O
?
BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV
-
-
?
colloidal chitin + H2O
?
-
from crab shell chitin
-
-
?
colloidal chitin + H2O
?
-
CHT1 is specific for chitin substrates
-
-
?
colloidal chitin + H2O
?
the enzyme is twice as active on colloidal chitin than on untreted chitin. It cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
high activity
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
100% activity
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
colloidal chitin + H2O
beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
-
-
?
colloidal chitin + H2O
GlcNAc + N,N'-diacetylchitobiose
-
-
-
-
?
colloidal chitin + H2O
GlcNAcbeta(1-4)GlcNAc + ?
-
major product
-
?
colloidal chitin + H2O
GlcNAcbeta(1-4)GlcNAc + ?
Thermochaetoides thermophila
-
major product
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
-
-
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
-
-
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
-
-
-
?
colloidal chitin + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + N-acetyl-beta-D-glucosamine
-
-
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
-
-
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
Thermochaetoides thermophila
-
-
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
-
-
major product
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
-
the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + ?
-
the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
prepared from commercial chitin
-
-
?
colloidal chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
prepared from commercial chitin
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + ?
from crab shell
isozyme ChiG shows unique activity and completely splits the dimer into monomers
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + ?
-
100% activity
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + ?
-
best substrate
the main product after hydrolysis of colloidal chitin
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + ?
-
100% activity
-
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine + ?
-
100% activity
-
-
?
colloidal chitin + H2O
N-acetyl-D-glucosamine + ?
-
-
-
-
?
colloidal chitin + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
colloidal chitin + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
colloidal chitin + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
main product, minor products (GlcNAc)2 and (GlcNAc)4 are produced in the molar ratio (GlcNAc)3:(GlcNAc)2:(GlcNAc)4 of 1:0.3:0.4
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
-
major product, plus some (GlcNAc)2 and (GlcNAc)4
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
-
?
glycol chitin + H2O
?
-
95% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
?
Hydrogenophilus hirschii KB-DZ44
-
95% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
?
-
92% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
?
-
92% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
?
89.4% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
?
Paenibacillus barengoltzii CAU904
89.4% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
N,N',N''-triacetylchitotriose + ?
-
-
a major product
-
?
glycol chitin + H2O
N,N',N''-triacetylchitotriose + ?
-
-
a major product
-
?
glycol chitin + H2O
N-acetyl-beta-D-glucosamine + ?
89.4% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
N-acetyl-beta-D-glucosamine + ?
-
29.8% activity compared to colloidal chitin
-
-
?
glycol chitin + H2O
N-acetylglucosamine + ?
-
tritiated and unlabeled
-
-
?
glycol chitosan + H2O
?
-
83% of the activity with colloidal chitin
-
-
?
glycol chitosan + H2O
?
-
90% activity compared to colloidal chitin
-
-
?
glycol chitosan + H2O
?
Hydrogenophilus hirschii KB-DZ44
-
90% activity compared to colloidal chitin
-
-
?
glycol chitosan + H2O
?
-
88% activity compared to colloidal chitin
-
-
?
glycol chitosan + H2O
?
-
88% activity compared to colloidal chitin
-
-
?
hexa-N-acetyl-chitohexaose + H2O
?
-
identification of the substrate interaction region of the chitin-binding domain of chitinase C
-
-
?
hexa-N-acetyl-chitohexaose + H2O
?
-
identification of the substrate interaction region of the chitin-binding domain of chitinase C
-
-
?
hexa-N-acetyl-chitohexaose + H2O
di-N-acetyl-chitobiose + tri-N-acetyl-chitotriose
di-N-acetyl-chitobiose is the main product, production of a small amount of tri-N-acetyl-chitotriose
-
-
?
hexa-N-acetyl-chitohexaose + H2O
di-N-acetyl-chitobiose + tri-N-acetyl-chitotriose
di-N-acetyl-chitobiose is the main product, production of a small amount of tri-N-acetyl-chitotriose
-
-
?
lactic acid-treated crab shell + H2O
?
-
13% of the activity with colloidal chitin
-
-
?
lactic acid-treated crab shell + H2O
?
-
18% of the activity with colloidal chitin
-
-
?
laminarin + H2O
?
-
the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin
-
-
?
laminarin + H2O
?
-
the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin
-
-
?
N,N',N'''-triacetyl-beta-D-chitotriose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'''-triacetyl-beta-D-chitotriose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O
3 N,N'-diacetylchitobiose
-
the enzyme hydrolyzes the second beta-1,4-glycosidic linkage from the non-reducing end of chitin oligosaccharide substrates, i.e. N,N',N'',N'''-tetraacetylchitotetraose, N,N',N'',N''',N''''-pentaacetylchitopentaose, or N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose, producing the beta-anomer of (GlcNAc)2
-
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O
3 N,N'-diacetylchitobiose
-
the enzyme hydrolyzes the second beta-1,4-glycosidic linkage from the non-reducing end of chitin oligosaccharide substrates, i.e. N,N',N'',N'''-tetraacetylchitotetraose, N,N',N'',N''',N''''-pentaacetylchitopentaose, or N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose, producing the beta-anomer of (GlcNAc)2, product analysis by MALDI-TOF mass spectrometry
-
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O
3 N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O
3 N,N'-diacetylchitobiose
-
-
major product
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
Q0WT03
the enzyme hydrolyzes from the non-reducing end
equal amounts of N,N'-diacetylchitobiose and N,N',N'',N'''-tetraacetylchitotetraose and a lesser amount of N,N',N''-triacetylchitotriose are produced in the early stage of the reaction
-
?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose
the enzyme hydrolyzes predominantly the second glycosidic linkage from the non-reducing end
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
?
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
?
-
the time course of hydrolysis exhibits distinct hydrolytic patterns between wild-type and mutants W275G and W397F. Wild-type enzyme initially hydrolyzes N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose to N,N',N'',N'''-tetraacetylchitotetraose and N,N'-diacetylchitobiose, and finally N,N'-diacetylchitobiose is formed as a major end product. W275G primarily creates N,N'-diacetylchitobiose to N,N',N'',N''',N''''-pentaacetylchitopentaose intermediates, and later N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose are formed as stable products. W397F initially produces (GlcNAc)1 to N,N',N'',N''',N''''-pentaacetylchitopentaose, and then the N,N',N''-triacetylchitotriose to N,N',N'',N''',N''''-pentaacetylchitopentaose are broken down to (GlcNAc)1 and N,N'-diacetylchitobiose end products
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + ?
ChiC shows rapid disappearance of the polymer and production of a continuum of odd- and even-numbered oligomers. ChiC is a nonprocessive endochitinase
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + ?
the polymer disappears very slowly, while the initially produced oligomers almost exclusively had even-numbered chain lengths in the 212 range. ChiA is a processive chitinase
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + ?
the polymer disappears very slowly, while the initially produced oligomers almost exclusively had even-numbered chain lengths in the 212 range. ChiB is a processive chitinase
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
-
-
OsChia1c almost exclusively generates (GlcNAc)3 and small amounts of (GlcNAc)2 and (GlcNAc)4, OsChia2b hydrolyzes the substrate to predominantely yield (GlcNAc)2
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
-
-
ChiA almost exclusively generates (GlcNAc)2 and (GlcNAc)4, ChiB and ChiC1 generated all three products
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
-
-
ChiA almost exclusively generates (GlcNAc)2 and (GlcNAc)4, ChiB and ChiC1 generated all three products
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
5 N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
5 N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + 4 H2O
5 N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
-
the enzyme liberates disaccharides from the reducing end
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + GlcNAc
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + GlcNAc
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + GlcNAc
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
Q0WT03
the enzyme hydrolyzed N,N',N'',N''',N''''-pentaacetylchitopentaose from the non-reducing end, docking simulation, detailed overview
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
Chi3
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
Chi3
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
the enzyme hydrolyzed N,N',N'',N''',N''''-pentaacetylchitopentaose at the third linkage from the non-reducing end
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
the enzyme hydrolyzes predominantly the second glycosidic linkage from the non-reducing end
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
poorly hydrolyzed by OsChia1c but efficiently hydrolyzed by OsChia2b to predominantely yield (GlcNAc)2
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
hydrolyzed at 27.8% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose. The initial alpha-anomer ratio of N,N',N'',N''',N''''-pentaacetylchitopentaose is 59%. After the hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose, only the alpha-anomer of N,N',N''-triacetylchitotriose is found after the reaction for 1 min
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
major product
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
Chi3
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
Chi3
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
the enzyme hydrolyzes predominantly the second glycosidic linkage from the non-reducing end
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
poorly hydrolyzed by OsChia1c but efficiently hydrolyzed by OsChia2b to predominantely yield (GlcNAc)2
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
-
-
major product
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
-
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
because the products (N-acetyl-beta-D-glucosamine)3 and N-acetyl-beta-D-glucosamine are rich in alpha- and beta-forms, respectively, (N-acetyl-beta-D-glucosamine)4 is supposed to bind to -2, -1, +1, and +2 subsites and -3, -2, -1, and +1 subsites
-
-
?
N,N',N''-triacetylchitotriose + 2 H2O
3 N-acetyl-D-glucosamine
-
-
-
?
N,N',N''-triacetylchitotriose + 2 H2O
3 N-acetyl-D-glucosamine
-
-
-
?
N,N',N''-triacetylchitotriose + 2 H2O
3 N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
-
the enzyme liberates disaccharides from the reducing end
-
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
the rate of beta-(N-acetyl-beta-D-glucosamine)3 hydrolysis is higher than that of alpha-(N-acetyl-beta-D-glucosamine)3. Because enzyme Ra-ChiC is an inverting enzyme, chitotriose is supposed to bind predominantly to -2, -1, and +1 subsites
the products (N-acetyl-beta-D-glucosamine)2 and N-acetyl-beta-D-glucosamine are rich in alpha- and beta-forms, respectively
-
?
N,N',N''-triacetylchitotriose + H2O
N,N'-diacetylchitobiose + N-acetyl-D-glucosamine
the rate of beta-(N-acetyl-beta-D-glucosamine)3 hydrolysis is higher than that of alpha-(N-acetyl-beta-D-glucosamine)3. Because enzyme Ra-ChiC is an inverting enzyme, chitotriose is supposed to bind predominantly to -2, -1, and +1 subsites
the products (N-acetyl-beta-D-glucosamine)2 and N-acetyl-beta-D-glucosamine are rich in alpha- and beta-forms, respectively
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
OsChia2b
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
chitobiose only
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
chitobiose only
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
chitobiose only
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
chitobiose only
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
N-acetylglucosamine only
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
N-acetylglucosamine and chitobiose, both isoenzymes
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
N-acetylglucosamine and chitobiose, both isoenzymes
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
-
3H labelled, only slightly attacked
-
?
N,N'-diacetylchitobiose + H2O
2 acetyl-D-glucosamine
-
-
-
?
N,N'-diacetylchitobiose + H2O
2 acetyl-D-glucosamine
-
high activity
-
-
?
N,N'-diacetylchitobiose + H2O
deacetylated chitooligosaccharides + acetate
-
-
-
?
N,N'-diacetylchitobiose + H2O
deacetylated chitooligosaccharides + acetate
-
-
-
?
N-acetylchito-oligosaccharides + H2O
?
-
endo-type cleavage mode
-
-
?
p-methoxyphenyl TMG-chitotriomycin + H2O
?
a synthetic compound and an enzyme inhibitor specific for insect and fungal b-N-acetylglucosaminidases
-
-
?
p-methoxyphenyl TMG-chitotriomycin + H2O
?
a synthetic compound and an enzyme inhibitor specific for insect and fungal b-N-acetylglucosaminidases
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
isoenzyme HoChiA
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
low activity, isoenzyme HoChiB
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
low activity, isoenzyme HoChiC
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
13% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi535
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
27% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi386
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
-
-
-
?
p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
?
-
30% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose
-
-
?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
N,N',N''-triacetylchitotriose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
N,N',N''-triacetylchitotriose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
N,N',N''-triacetylchitotriose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
chit37 and chit42
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobiose + H2O
p-nitrophenol + N,N'-diacetylchitobiose
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
N,N'-diacetylchitobiose + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
N-acetyl-D-glucosamine + p-nitrophenol
-
-
-
-
?
p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
N-acetyl-D-glucosamine + p-nitrophenol
-
-
-
-
?
partially deacetylated chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
-
higher activity toward chitosan 7B, a 62% deacetylated chitosan, than toward the highly deacetylated chitosan substrates: chitosan EL, 9B, 8B and 10
-
-
?
partially deacetylated chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
-
higher activity toward chitosan 7B, a 62% deacetylated chitosan, than toward the highly deacetylated chitosan substrates: chitosan EL, 9B, 8B and 10
-
-
?
partially N-acetylated chitosan + H2O
?
-
variuos degrees of acetylation
-
-
?
partially N-acetylated chitosan + H2O
?
-
variuos degrees of acetylation
-
-
?
partially N-acetylated chitosan + H2O
?
-
-
-
?
partially N-acetylated chitosan + H2O
?
-
-
-
?
partially N-acetylated chitosan + H2O
?
-
for the class I enzyme OsChia1cDELTAChBD, the reducing end residue is preferentially acetylated, although the specificity is not absolute. The nearest neighbor to the acetylated reducing end residue is specifically acetylated. The nonreducing end residue produced by the class I enzyme is exclusively acetylated, although there is a low but significant preference for deacetylated units at the nearest neighbor to the nonreducing end. The three contiguous subsites (-2), (-1), and (+1) of the class I enzyme are specific to three consecutive GlcNAc residues of the substrate
-
-
?
partially N-acetylated chitosan + H2O
?
-
the reducing end residue of the enzymatic products obtained by the class III enzyme OsChib1a is exclusively acetylated, whereas both acetylated and deacetylated units are found at the nearest neighbor to the reducing end residue. Both acetylated and deacetylated units are also found at the nonreducing end residue and its nearest neighbor of the class III enzyme products. Only subsite (-1) among the contiguous subsites (-2) to (+2) of the class III enzyme is specific to an acetylated residue
-
-
?
partially N-acetylated chitosan + H2O
?
-
chitosan 7B, 8B, 9B and 10B
-
-
?
powdery chitin + H2O
?
15.4% of the activity with colloidal chitin
-
-
?
powdery chitin + H2O
?
Thermochaetoides thermophila
32.2% of the activity with colloidal chitin
-
-
?
powdery chitin + H2O
N,N'-diacetylchitobiose
-
-
-
?
solid beta-chitin + H2O
N,N'-diacetylchitobiose
-
the enzyme hydrolyzes solid beta-chitin producing only N,N'-diacetylchitobiose
-
-
?
solid beta-chitin + H2O
N,N'-diacetylchitobiose
-
the enzyme hydrolyzes solid beta-chitin producing only N,N'-diacetylchitobiose, product analysis by MALDI-TOF mass spectrometry
-
-
?
xylan + H2O
?
-
40% activity compared to colloidal chitin
-
-
?
xylan + H2O
?
-
40% activity compared to colloidal chitin
-
-
?
additional information
?
-
essential role of chitinase in the life cycle of Acanthocheilonema viteae
-
-
?
additional information
?
-
essential role of chitinase in the life cycle of Acanthocheilonema viteae
-
-
?
additional information
?
-
-
essential role of chitinase in the life cycle of Acanthocheilonema viteae
-
-
?
additional information
?
-
no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose
-
-
?
additional information
?
-
-
no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose
-
-
?
additional information
?
-
no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose
-
-
?
additional information
?
-
-
colloidal chitin is a better substrate for the enzyme induction than the mycelia of the saprophytic fungi, overview
-
-
?
additional information
?
-
-
no activity with 4-methylumbelliferyl N-acetylglucosamine
-
-
?
additional information
?
-
Q0WT03
recombinant AtChiC hydrolyzes N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates, mode of action toward the oligosaccharide substrates, overview. The enzyme catalyzes transglycosylation reaction in a low efficiency
-
-
?
additional information
?
-
-
recombinant AtChiC hydrolyzes N-acetylglucosamine oligomers producing dimers from the non-reducing end of the substrates, mode of action toward the oligosaccharide substrates, overview. The enzyme catalyzes transglycosylation reaction in a low efficiency
-
-
?
additional information
?
-
-
the enzyme also performs transglycosylation reactions
-
-
?
additional information
?
-
-
the enzyme also performs transglycosylation reactions
-
-
?
additional information
?
-
ChiA functions as a glycosylphosphatidylinositol-anchored chitinase at the sites where cell wall remodeling and/or cell wall maturation actively take place
-
-
?
additional information
?
-
-
ChiA functions as a glycosylphosphatidylinositol-anchored chitinase at the sites where cell wall remodeling and/or cell wall maturation actively take place
-
-
?
additional information
?
-
ChiA functions as a glycosylphosphatidylinositol-anchored chitinase at the sites where cell wall remodeling and/or cell wall maturation actively take place
-
-
?
additional information
?
-
-
endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc
-
-
?
additional information
?
-
-
endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose
-
-
?
additional information
?
-
-
endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc
-
-
?
additional information
?
-
-
endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose
-
-
?
additional information
?
-
-
processing mechanism, mode of action of the enzyme, overview. The enzyme also shows transglycosylation activity
-
-
?
additional information
?
-
the class I chitinase inhibits the growth of Penicillium roqueforti IBT 5426, overview
-
-
?
additional information
?
-
-
ChiCW exhibits antifungal activity against Alternaria brassicicola, Botrytis elliptica, and Colletotrichum gloeosporioides with an effective concentration of 0.00325 mg/ml, and acts synergistically with fungicides cyprodinil and fludioxonil, and tebuconazole to inhibit fungal conidial germinations, ChiCW used alone does not inhibit conidial germination of Alternaria longipes, detailed overview
-
-
?
additional information
?
-
-
substrate specificity, overview. No activity with carboxymethyl cellulose
-
-
?
additional information
?
-
-
ChiCW exhibits antifungal activity against Alternaria brassicicola, Botrytis elliptica, and Colletotrichum gloeosporioides with an effective concentration of 0.00325 mg/ml, and acts synergistically with fungicides cyprodinil and fludioxonil, and tebuconazole to inhibit fungal conidial germinations, ChiCW used alone does not inhibit conidial germination of Alternaria longipes, detailed overview
-
-
?
additional information
?
-
-
substrate specificity, overview. No activity with carboxymethyl cellulose
-
-
?
additional information
?
-
-
strain MY75 has the ability to inhibit the growth of Gibberella saubinetii and Aspergillus niger, two major pathogenic fungi in agriculture, and to restrain their spore germination completely, due to chitinase activity, overview
-
-
?
additional information
?
-
-
strain MY75 has the ability to inhibit the growth of Gibberella saubinetii and Aspergillus niger, two major pathogenic fungi in agriculture, and to restrain their spore germination completely, due to chitinase activity, overview
-
-
?
additional information
?
-
the enzyme can not hydrolyze glycol chitin or glycol chitosan
-
-
?
additional information
?
-
-
the enzyme can not hydrolyze glycol chitin or glycol chitosan
-
-
?
additional information
?
-
-
the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc
-
-
?
additional information
?
-
Bacillus sp. (in: Bacteria) DAU101
the enzyme can not hydrolyze glycol chitin or glycol chitosan
-
-
?
additional information
?
-
-
the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc
-
-
?
additional information
?
-
recombinant His6-tagged SCChi-1 shows the highest lytic activity on colloidal chitin and acidic chitosan
-
-
?
additional information
?
-
no activity with 4-methylumbelliferyl-(GlcNAc)
-
-
?
additional information
?
-
-
no activity with 4-methylumbelliferyl-(GlcNAc)
-
-
?
additional information
?
-
-
isozyme chiA Nima shows high antibacterial activity against Enterobacter cloacae, Escherichia coli, Staphylococcus aureus and Staphylococcus xylosus
-
-
?
additional information
?
-
-
recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum
-
-
?
additional information
?
-
-
Chi3 acts as an endohydrolase, it cleaves chitin trimers into monomers and dimers and cleaves chitin tetramer to hexamer into a mixture of monomers, dimers, and trimers
-
-
?
additional information
?
-
Chi-h plays a role in the chitin degradation during the molting process
-
-
?
additional information
?
-
-
Chi-h plays a role in the chitin degradation during the molting process
-
-
?
additional information
?
-
CHI1 plays a role in the plant's defense system, CHI1 and its derivative with one chitin-binding domain show anti-fungal activities against phytopathogens, i.e. Colletotrichum truncatum, Colletotrichum acutatum, Botrytis cinerea, and Ascochyta rabiei, and inhibit spore germination of Colletotrichum truncatum, overview. BjCHI1 agglutinates Gram-negative bacteria
-
-
?
additional information
?
-
-
CHI1 plays a role in the plant's defense system, CHI1 and its derivative with one chitin-binding domain show anti-fungal activities against phytopathogens, i.e. Colletotrichum truncatum, Colletotrichum acutatum, Botrytis cinerea, and Ascochyta rabiei, and inhibit spore germination of Colletotrichum truncatum, overview. BjCHI1 agglutinates Gram-negative bacteria
-
-
?
additional information
?
-
the native enzyme is involved in cold acclimation and freezing tolerance, recombinant enzymes do not show anti-freeze activity in the expression systems, overview
-
-
?
additional information
?
-
-
recombinant CpCHI shows antibacterial activity in which 50% of Escherichia coli is inhibited by a 0.0025 mM concentration of the enzyme. Antifungal activity: the spore germination of Alternaria brassicicola can be completely inhibited by a 76 nM level of recombinant CpCHI
-
-
?
additional information
?
-
-
purified enzyme is able to lyse the cell wall of Rhizopus oligosporus, Mucor miehei, Penicillium sp., Streptomyces phaerochromogenes, and to form protoplasts
-
-
?
additional information
?
-
-
purified enzyme is able to lyse the cell wall of Rhizopus oligosporus, Mucor miehei, Penicillium sp., Streptomyces phaerochromogenes, and to form protoplasts
-
-
?
additional information
?
-
may be involved in the biocontrol mechanism of Chaetomium globosum
-
-
?
additional information
?
-
-
may be involved in the biocontrol mechanism of Chaetomium globosum
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
-
no activity with colloidal chitin
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
no activity with colloidal chitin
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
the recombinant isozyme ChiF has no chitinolytic activity
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine
-
-
?
additional information
?
-
-
isozymes ChiA, ChiB, and ChiC degrade squid pen beta-chitin to a higher degree than shrimp shell or crab shell alpha-chitin, isozyme specificity towards soluble substrates, overview. No activity with 4-nitrophenyl-(GlcNAc)3. General sctivity of isozymes in descending order: ChiA, ChiC, ChiB
-
-
?
additional information
?
-
-
chitinase Chi1 plays a role in the infection of nematodes by the fungus
-
-
?
additional information
?
-
not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside
-
-
?
additional information
?
-
-
not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside
-
-
?
additional information
?
-
not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside
-
-
?
additional information
?
-
-
not active toward 4-methylumbelliferyl-N-acetylglucosamine or 4-methylumbelliferyl-beta-D-N-N'-diacetylchitobioside
-
-
?
additional information
?
-
-
no activity with 4-methylumbelliferyl-(GlcNAc)
-
-
?
additional information
?
-
-
the enzyme ChiEn1 does not hydrolyze 4-nitrophenyl N-acetyl-beta-D-glucosaminide, carboxymethyl cellulose, laminarin, chitobiose, chitohexaose, insoluble chitin powder, colloidal chitin, and soluble glycol chitosan
-
-
?
additional information
?
-
-
the enzyme ChiEn1 does not hydrolyze 4-nitrophenyl N-acetyl-beta-D-glucosaminide, carboxymethyl cellulose, laminarin, chitobiose, chitohexaose, insoluble chitin powder, colloidal chitin, and soluble glycol chitosan
-
-
?
additional information
?
-
residue Glu155 is involved in the catalytic reaction
-
-
?
additional information
?
-
-
residue Glu155 is involved in the catalytic reaction
-
-
?
additional information
?
-
-
the class III enzymes of cultivars Galia and Bredor react differently to Fusarium oxysporum f. sp. melonis attack at roots, cultivar Bredor is resistant to the pathogen, while Galia is not, phenotype, overview
-
-
?
additional information
?
-
enzyme exhibits transglycosylation activity
-
-
?
additional information
?
-
-
enzyme exhibits transglycosylation activity
-
-
?
additional information
?
-
-
the enzyme is involved and effective in defense response to pathogens, gene VCH3 of the arbuscular mycorrhizal fungus Glomus versiforme, colonizing in Vitis amurensis, is transcriptionally induced in defense response of the grapevine host against the root-knot nematode Meloidogyne incognita, colonization of grapevine has no effects on fungus growth nor on root growths
-
-
?
additional information
?
-
no activity with 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside, and glycol chitin
-
-
?
additional information
?
-
-
no activity with 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside, and glycol chitin
-
-
?
additional information
?
-
-
the enzyme acts toward chitin polysaccharide chain through a cooperative action of the two endo-splitting catalytic domains, recognizing two contiguous A-units at subsites -2 and -1
-
-
?
additional information
?
-
the isozyme APAP1 may play a role in life span regulation and is indispensable for the hormonally induced diapause termination
-
-
?
additional information
?
-
the isozyme APAP1 may play a role in life span regulation and is indispensable for the hormonally induced diapause termination
-
-
?
additional information
?
-
-
the isozyme APAP1 may play a role in life span regulation and is indispensable for the hormonally induced diapause termination
-
-
?
additional information
?
-
-
BcChi-A is optimized for shorter substrates than class II enzymes
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiA
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiB
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiC
-
-
?
additional information
?
-
AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity
-
-
?
additional information
?
-
-
AMCase is important in cytokine Th2-mediated inflammation and is implicated in asthma and allergic disease, inhibition of AMCase results in increased airway inflammation and airway hyperresponsiveness, the enzyme's transglycosylation activity probably is a mechanism in vivo for feedback regulation of the chitinase activity
-
-
?
additional information
?
-
-
SI-CLP shows no chitinase due to the lack of chitin-binding domain, but cytokine activity, the enzyme directly interacts with stabilin-1, which is involved in intracellular sorting of SI-CLP
-
-
?
additional information
?
-
substrate specificity, overview, AMCase also shows low intrinsic transglycosylation activity, product analysis
-
-
?
additional information
?
-
-
substrate specificity, overview, AMCase also shows low intrinsic transglycosylation activity, product analysis
-
-
?
additional information
?
-
-
acidic mammalian chitinase plays an important role in the pathogenesis of Th2-mediated diseases and antiparasite responses, it regulates epithelial cell apoptosis via a chitinolytic-independent mechanism, AMCase induces phosphorylations by Akt activation, overview
-
-
?
additional information
?
-
AMCase is a mammalian chitinase that is implicated in allergic asthma
-
-
?
additional information
?
-
-
chitinases are hydrolases capable of hydrolyzing the abundant natural polysaccharide chitin, the linear polymer of beta-1,4-linked N-acetylglucosamine, by releasing terminal GlcNAc monomers, in combination with chitinases cleaving internal sites within the chitin chain
-
-
?
additional information
?
-
-
development of a sensitive assay for the detection of chitooligosaccharide reaction products released by chitinases, based on fluorescent detection, following chemical labeling by 2-aminobenzoic acid, overview
-
-
?
additional information
?
-
modelling of (GlcNAc)2 ligand binding at the active site
-
-
?
additional information
?
-
-
modelling of (GlcNAc)2 ligand binding at the active site
-
-
?
additional information
?
-
-
synthesis of three fluorogenic chitobiosyl derivatives, modified at the non-reducing 4'-OH with, either a methyl, an isopropyl or a cyclohexylmethyl substituent, overview. The 4'-capped 4-methylumbelliferyl chitobiosides are hydrolysed, unmodified chitobiosyl-4-methylumbelliferone undergoes transglycosylation. No activity with N-acetylglucosaminyl-4-methylumbelliferone
-
-
?
additional information
?
-
purified chitinase exerts broad-spectrum antifungal activity against Botrytis cinerea (blight of tobacco), Pestalotia theae (leaf spot of tea), Bipolaris oryzae (brown spot of rice), Alternaria sp. (grain discoloration of rice), Curvularia lunata (leaf spot of clover) and Rhizoctonia solani (sheath blight of rice)
-
-
?
additional information
?
-
-
purified chitinase exerts broad-spectrum antifungal activity against Botrytis cinerea (blight of tobacco), Pestalotia theae (leaf spot of tea), Bipolaris oryzae (brown spot of rice), Alternaria sp. (grain discoloration of rice), Curvularia lunata (leaf spot of clover) and Rhizoctonia solani (sheath blight of rice)
-
-
?
additional information
?
-
the class I chitinase inhibits the growth of Penicillium roquefortii, overview
-
-
?
additional information
?
-
the class I chitinase inhibits the growth of Penicillium roquefortii, overview
-
-
?
additional information
?
-
-
the class I chitinase inhibits the growth of Penicillium roquefortii, overview
-
-
?
additional information
?
-
-
no substrate: (GlcNAc)4, (GlcNAc)3, and (GlcNAc)2
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose
-
-
?
additional information
?
-
Hydrogenophilus hirschii KB-DZ44
-
the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose
-
-
?
additional information
?
-
-
colloidal chitin is a better substrate for the enzyme induction than the mycelia of the saprophytic fungi, overview
-
-
?
additional information
?
-
LlChi18A has a shallow substrate-binding groove characteristic of nonprocessive endochitinases. LlChi18A is able to hydrolyse both chitin oligomers and artificial substrates, with no sign of processivity. Substrate specificity and binding preferences, overview
-
-
?
additional information
?
-
-
LlChi18A has a shallow substrate-binding groove characteristic of nonprocessive endochitinases. LlChi18A is able to hydrolyse both chitin oligomers and artificial substrates, with no sign of processivity. Substrate specificity and binding preferences, overview
-
-
?
additional information
?
-
-
Leishmania chitinase is a multifunctional virulence factor that benefits the parasite throughout its entire life cycle and assists its survival in Lutzomyia longipalpis. Specifically, this enzyme enables the parasites to colonize the anterior midgut of the sand fly more quickly, modify the sand fly stomodeal valve and affect its blood feeding, all of which combine to enhance transmission
-
-
?
additional information
?
-
enzyme efficiently degrades cell wall chitin of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici and Phytophthora sojae
-
-
?
additional information
?
-
-
enzyme efficiently degrades cell wall chitin of the phytopathogenic Rhizoctonia solani, Fusarium oxysporum, Sclerotinia sclerotiorum, Valsa sordida, Septoria tritici and Phytophthora sojae
-
-
?
additional information
?
-
isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
additional information
?
-
isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
additional information
?
-
-
isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
additional information
?
-
isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
additional information
?
-
isoform Chi35 is practically inert to N,N',N''-triacetyl-beta-D-chitotriose
-
-
?
additional information
?
-
-
the enzyme exhibits exochitinase and beta-N-acetyl hexosaminidase activities releasing chitobiose and N-acetylglucosamine as end products, respectively
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi386
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi535
-
-
?
additional information
?
-
-
no substrate: flake chitin, chitosan with degree of acetylation 96.9, carboxymethyl cellulose. No exochitinase activity for both isoform Chi-56 and Chi-64
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin
-
-
?
additional information
?
-
the enzyme is not active with synthetic 4-nitrophenyl-linked glycosidic substrates, overview
-
-
?
additional information
?
-
-
the enzyme is not active with synthetic 4-nitrophenyl-linked glycosidic substrates, overview
-
-
?
additional information
?
-
-
simvastatin promotes Th2-type responses through the induction of Ym1 in dendritic cells, mechanism, overview
-
-
?
additional information
?
-
-
Ym1 is a lectin with heparin-binding characteristics and belongs to the chitinase family, but does not show chitinase activity
-
-
?
additional information
?
-
-
AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview
-
-
?
additional information
?
-
-
native AMCase from the BALB/c mouse stomach shows higher chitinolytic activity compared with the hydrolytic effects of the recombinant AMCase protein
-
-
?
additional information
?
-
-
AMCase plays an important role in both the efficient hydrolysis of chitin and antifungal activity, AMCase shows antifungal activity against Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains by inhibition of hyphal growth activity, potency of native and recombinant enzymes, overview
-
-
?
additional information
?
-
-
native AMCase from the BALB/c mouse stomach shows higher chitinolytic activity compared with the hydrolytic effects of the recombinant AMCase protein
-
-
?
additional information
?
-
-
the enzyme exhibits weak activity toward polymeric substrates and significant activity toward (GlcNAc)n (n=4-6). The anomeric form of the products indicate an inverting enzyme
-
-
?
additional information
?
-
-
no activity with N,N',N''-triacetylchitotriose
-
-
?
additional information
?
-
-
the enzyme is a a retaining enzyme. No activity with chitotetraose. Anomeric forms of the reaction products
-
-
?
additional information
?
-
-
when cleaved by a proteolytic enzyme, the 20000 Da product can retain high chitinolytic activity
-
-
?
additional information
?
-
Niallia circulans No. 4.1
-
when cleaved by a proteolytic enzyme, the 20000 Da product can retain high chitinolytic activity
-
-
?
additional information
?
-
NtChitIV plays a role in defense response against pathogen infection
-
-
?
additional information
?
-
-
NtChitIV plays a role in defense response against pathogen infection
-
-
?
additional information
?
-
the enzyme hydrolyzes N-acetylglucosamine oligosaccharides, predominantly the second glycosidic linkage from the non-reducing end. And NtChiV hydrolyzes partially N-acetylated chitosan non-processively. NtChiV does catalyze a transglycosylation reaction, but the extent is negligible over the hydrolysis reaction. Substrate recognition and mode of action, overview
-
-
?
additional information
?
-
-
the enzyme hydrolyzes N-acetylglucosamine oligosaccharides, predominantly the second glycosidic linkage from the non-reducing end. And NtChiV hydrolyzes partially N-acetylated chitosan non-processively. NtChiV does catalyze a transglycosylation reaction, but the extent is negligible over the hydrolysis reaction. Substrate recognition and mode of action, overview
-
-
?
additional information
?
-
-
the enzyme shows antifungal activity against Fusarium moniliforme, overview
-
-
?
additional information
?
-
-
the enzyme shows antifungal activity against Fusarium moniliforme, overview
-
-
?
additional information
?
-
-
the enzyme may play a role in digestion and defense
-
-
?
additional information
?
-
-
the class III enzyme OsChib1a might act toward an endogenous complex carbohydrate containing GlcNAc residue
-
-
?
additional information
?
-
-
the target of the class I enzyme OsChia1cDELTAChBD might be a consecutive GlcNAc sequence probably in the cell wall of fungal pathogen
-
-
?
additional information
?
-
-
OsChia1b shows no activity against N-acetylchitotriose, and crystalline beta-chitin is a poor substrate. OsChia1b substrate binding specificity depends on the N-acetyl groups of the substrate. About 80% of added OsChia1b binds to colloidal chitin, whereas less than 30% of added OsChia1b binds to chitosan 7B, with 70% deacetylated chitin, avicel, and acid-swollen cellulose, less than 10% of OsChia1b binds to beta-1,3-glucan, curdlan
-
-
?
additional information
?
-
the enzyme is inactive towards tri-N-acetyl-chitotriose and insoluble chitin substrates like alpha-chitin, beta-chitin, and colloidal chitin
-
-
?
additional information
?
-
-
the enzyme is inactive towards tri-N-acetyl-chitotriose and insoluble chitin substrates like alpha-chitin, beta-chitin, and colloidal chitin
-
-
?
additional information
?
-
no activity towards beta-D-N,N'-diacetylchitobiose, 4-nitrophenyl N-acetylglucosamine, carboxmethyl cellulose and chitosan
-
-
?
additional information
?
-
-
no activity towards beta-D-N,N'-diacetylchitobiose, 4-nitrophenyl N-acetylglucosamine, carboxmethyl cellulose and chitosan
-
-
?
additional information
?
-
no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside
-
-
?
additional information
?
-
the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose
-
-
?
additional information
?
-
the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside
-
-
?
additional information
?
-
Paenibacillus barengoltzii CAU904
no activity towards beta-D-N,N'-diacetylchitobiose, 4-nitrophenyl N-acetylglucosamine, carboxmethyl cellulose and chitosan
-
-
?
additional information
?
-
Paenibacillus barengoltzii CAU904
no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside
-
-
?
additional information
?
-
Paenibacillus barengoltzii CAU904
the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose
-
-
?
additional information
?
-
Paenibacillus barengoltzii CAU904
the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside
-
-
?
additional information
?
-
thin layer chromatography analysis of the reaction products, substrate specificity, overview. No activity with glycol chitosan
-
-
?
additional information
?
-
-
thin layer chromatography analysis of the reaction products, substrate specificity, overview. No activity with glycol chitosan
-
-
?
additional information
?
-
no activity with powdered chitin, chitosan, beta-1,3-glucan, cellulose and xylan
-
-
?
additional information
?
-
-
the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose
-
-
?
additional information
?
-
-
the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose
-
-
?
additional information
?
-
-
the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme
-
-
?
additional information
?
-
-
exoenzyme, no activity with cellulose and carboxymethyl cellulose
-
-
?
additional information
?
-
-
exoenzyme, no activity with cellulose and carboxymethyl cellulose
-
-
?
additional information
?
-
-
the chitinase shows a wide substrate specificity for degrading alpha-chitin of shrimp and crab shell and beta-chitin of squid pen, substrate specificity silver croaker chitinase preferentially hydrolyzes the 2nd glycosidic link from the non-reducing end of N-acetylglucosaminyl oligomers, no activity with 4-nitrophenyl-GlcNAc, overview
-
-
?
additional information
?
-
-
the enzyme exerts a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii
-
-
?
additional information
?
-
-
4-nitrophenyl-beta-N,N'-diacetylchitobioside shows no measurable activity in whole fly homogenates, substrate concentration of 22 mM in acetate phospate buffer used
-
-
?
additional information
?
-
-
the enzyme plays a role in pathogen defense
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose
-
-
?
additional information
?
-
-
chitinase and beta-1,3-glucanase are involved in inhibition of Rhizoctonia solani but not Phytophthora capsici
-
-
?
additional information
?
-
-
chitinase and beta-1,3-glucanase are involved in inhibition of Rhizoctonia solani but not Phytophthora capsici
-
-
?
additional information
?
-
-
the LysM domains contribute significantly to the antifungal activity of PrChi-A through their binding activity to chitin in the cell wall of fungi
-
-
?
additional information
?
-
-
chitinase is not important for the growth of Pyrococcus furiosus
-
-
?
additional information
?
-
no activity toward 4-nitrophenyl-N-acetylglucosamine and chitooligomers smaller than chitotetraose
-
-
?
additional information
?
-
-
no activity toward 4-nitrophenyl-N-acetylglucosamine and chitooligomers smaller than chitotetraose
-
-
?
additional information
?
-
-
isozyme ChitIII-1 is involved in symbiosis ontogenesis in lateral roots and show enhanced expression in pre-mycorrhizal stage, isozyme ChitIII-1 is not expressed in principal roots, while isozyme ChitIII-2 is not regulated in all roots, overview
-
-
?
additional information
?
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identification of five substrate-binding subsites in the enzyme based on crystal structures, enzyme binding specificity, NMR spectroscopy analysis: two ligands, chitobiose and a peptide-glycan fragment, N-acetylglucosaminyl-(beta-1,4)-N-acetylmuramyl-L-alanyl-D-isoglutamine are used for NMR titration experiments: The mutant enzymes except Glu162 mutants E162A, E162Q, E162D, and E162N do not hydrolyze the N-acetyl-beta-D-glucosamine oligomers at all
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?
additional information
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identification of five substrate-binding subsites in the enzyme based on crystal structures, enzyme binding specificity, NMR spectroscopy analysis: two ligands, chitobiose and a peptide-glycan fragment, N-acetylglucosaminyl-(beta-1,4)-N-acetylmuramyl-L-alanyl-D-isoglutamine are used for NMR titration experiments: The mutant enzymes except Glu162 mutants E162A, E162Q, E162D, and E162N do not hydrolyze the N-acetyl-beta-D-glucosamine oligomers at all
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?
additional information
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partially O-methylated methyl chitobiosides
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?
additional information
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Salmonella enterica typhimurium
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the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids
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?
additional information
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Salmonella enterica typhimurium SL1344
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the purified enzyme hydrolyzes 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside, and carboxymethyl-Chitin-Remazol-Brilliant-Violet, but does not act on 4-nitrophenyl N-acetyl-beta-D-glucosaminide, peptidoglycan or 4-nitrophenyl beta-D-cellobioside. Hydrolysis occurs with the retention of configuration and the enzyme acts on only the beta-anomers of chitooligosaccharide substrates. The enzyme also released N-acetyllactosamine disaccharide from Galbeta1,4GlcNAcbeta-O-(CH2)8CONH(CH2)2NHCO-tetramethylrhodamine, a model substrate for LacNAc terminating glycoproteins and glycolipids
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additional information
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the enzyme hydrolyzes randomly beta-14,-N-acetylglucosamine linkages in the chitin polymer, and preferentially hydrolyzes the second glycosidic link from the nonreducing end of the N-acetylglucosamine oligomers
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additional information
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
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wild-type ChiA and mutant ChiAW167A substrate specificity, product determinations, overview, the enzyme also performs transglycosylation with chito-oligosaccharides as acceptors, overview
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additional information
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wild-type ChiA and mutant ChiAW167A substrate specificity, product determinations, overview, the enzyme also performs transglycosylation with chito-oligosaccharides as acceptors, overview
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additional information
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isozyme ChiA Nima shows high antibacterial activity against Enterobacter cloacae, Escherichia coli, Staphylococcus aureus and Staphylococcus xylosus, overview
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additional information
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aromatic residues in the catalytic center of chitinase A affect processivity, enzyme activity, and biomass converting efficiency. Progressivity of isozymes is dependent on the substrate, the processive mechanism is essential for an efficient conversion of crystalline substrates but comes at a large cost in terms of intrinsic enzyme speed, overview
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?
additional information
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the enzyme is also active with chitooligosachharides from GlnNAc to GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc, overview
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?
additional information
?
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the enzyme is also active with chitooligosachharides from GlnNAc to GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc, overview
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?
additional information
?
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aromatic residues in the catalytic center of chitinase A affect processivity, enzyme activity, and biomass converting efficiency. Progressivity of isozymes is dependent on the substrate, the processive mechanism is essential for an efficient conversion of crystalline substrates but comes at a large cost in terms of intrinsic enzyme speed, overview
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?
additional information
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CHI60 possesses a dual mode of catalysis with both exo- and endo-type activities allowing the enzyme to work efficiently on various substrate types
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additional information
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CHI60 possesses a dual mode of catalysis with both exo- and endo-type activities allowing the enzyme to work efficiently on various substrate types
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?
additional information
?
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the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide
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?
additional information
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the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide
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?
additional information
?
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the enzyme plays a role in pathogen defense
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?
additional information
?
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chitin substrate specificities of recombinant isozymes, overview
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?
additional information
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the enzyme is not capable of releasing 4-methylumbelliferyl from artificial chitooligosaccharides
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?
additional information
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the enzyme is not capable of releasing 4-methylumbelliferyl from artificial chitooligosaccharides
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?
additional information
?
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the enzyme plays a role in pathogen defense
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?
additional information
?
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chitin substrate specificities of recombinant isozymes, overview
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additional information
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substrate specificity, overview
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additional information
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chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide
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?
additional information
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no activity with 4-nitrophenyl-GlcNAc
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?
additional information
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chitinase A is an endo-type enzyme. Chitinase A participates in protoplast formation from Schizophyllum commune mycelia. No activity with N,N'-diacetylchitobiose, N,N',N''-triacetylchitotriose and p-nitrophenyl-beta-D-N-acetylglucosaminide
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additional information
?
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no activity with 4-nitrophenyl-GlcNAc
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?
additional information
?
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no activity with 4-nitrophenyl-GlcNAc
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?
additional information
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the bacterial chitinase specifically activates protease-activated receptor-2 and increases intracellular Ca2+ content and signalling in human airway epithelial NHBE and H-292 cells, which is inhibited by phospholipase A2 inhibitor U73122 and IP3 receptor inhibitor 2-APB, overview
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?
additional information
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the chitinase C shows antifungal activities against Aspergillus niger and Candida albicans. Streptomyces sp. strain DA11 is associated with South China sponge Craniella australiensis and might be involved in chitin degradation and antifungal defense of the sponge via its chitinase activity
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additional information
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colloidal chitin is a better substrate for the enzyme induction than the mycelia of the saprophytic fungi, overview
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?
additional information
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no activity with mycelia of Mucor hiemalis strain Bu 10 and Trichoderma harzianum strain Mn 75
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?
additional information
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colloidal chitin is a better substrate for the enzyme induction than the mycelia of the saprophytic fungi, overview
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?
additional information
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no activity with mycelia of Mucor hiemalis strain Bu 10 and Trichoderma virens strain Z 49
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?
additional information
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TmChi does not hydrolyze chitobiose, and chitotriose
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?
additional information
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no substrate: 4-nitrophenyl-(GlcNAc)
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?
additional information
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no substrate: 4-nitrophenyl-N-acetyl-D-glucosamine
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?
additional information
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no substrate: 4-nitrophenyl-N-acetyl-D-glucosamine
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?
additional information
?
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activity towards colloidal chitin and acetylated chitosan is minimal. The enzyme does not use N,N',N''-triacetyl-beta-D-chitotriose and RNase B as substrates
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?
additional information
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the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide
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?
additional information
?
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the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide
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?
additional information
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Thermobifida fusca ATCC BAA-629
the enzyme shows no activity with 4-nitrophenyl N-acetyl-beta-D-glucosaminide
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?
additional information
?
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Thermochaetoides thermophila
no substrate: 4-nitrophenyl-(GlcNAc)
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?
additional information
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Thermochaetoides thermophila
no substrate: 4-nitrophenyl-N-acetyl-D-glucosamine
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?
additional information
?
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essential role of E172, D170 and E145 in the catalytic mechanism of Chit33
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?
additional information
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essential role of E172, D170 and E145 in the catalytic mechanism of Chit33
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?
additional information
?
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essential role of E172, D170 and E145 in the catalytic mechanism of Chit33
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?
additional information
?
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multiple regulatory proteins are involved in Chi46 expression
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?
additional information
?
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multiple regulatory proteins are involved in Chi46 expression
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?
additional information
?
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the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose, beta-D-N,N',N''-triacetylchitotriose, alpha-chitin and beta-chitin
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?
additional information
?
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the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose, beta-D-N,N',N''-triacetylchitotriose, alpha-chitin and beta-chitin
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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the class I chitinase inhibits the growth of Penicillium roquefortii, overview
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?
additional information
?
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substrate (GlcNAc)6 prefers subsites -2 to +2 over subsites -3 to +2. Substrate (GlcNAc)5 only requires subsites -2 to +2. The binding of the chitooligosaccharides to the enzyme essentially occurs in compulsory fashion
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?
additional information
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no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose, N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose. Activity with N,N',N'',N''',N''''-pentaacetylchitopentaose is 0.1% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose
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?
additional information
?
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no hydrolysis of p-nitrophenyl-N,N'-diacetylchitobiose, N,N'-diacetylchitobiose and N,N',N''-triacetylchitotriose. Activity with N,N',N'',N''',N''''-pentaacetylchitopentaose is 0.1% of the activity with N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose
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?
additional information
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type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity
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?
additional information
?
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type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity
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?
additional information
?
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type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity
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?
additional information
?
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type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity
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?
additional information
?
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the secreted protease of Xylella fastidiosa also exhibits chitinase and beta-1,3-glucanase activity
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?
additional information
?
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substrate specificity: the secreted serine- and metallo-protease of Xylella fastidiosa also exhibits chitinase and beta-1,3-glucanase activity, overview
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?
additional information
?
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enzyme activity on the modified chitopentaose substrate GlcN-GlcNAc4, which contains a non-acetylated glucosamine residue at the nonreducing end, and on the modified chitotetraose substrate TMG-chitotriomycin-pMP, which contains a N-trimethylamino glucosamine (TMG) residue at the non-reducing end, and a p-methyoxyphenyl protecting group at the anomeric position, cleavage product is TMG-GlcNAc2, MALDI-TOF mass spectrometry, overview
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?
additional information
?
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enzyme activity on the modified chitopentaose substrate GlcN-GlcNAc4, which contains a non-acetylated glucosamine residue at the nonreducing end, and on the modified chitotetraose substrate TMG-chitotriomycin-pMP, which contains a N-trimethylamino glucosamine (TMG) residue at the non-reducing end, and a p-methyoxyphenyl protecting group at the anomeric position, cleavage product is TMG-GlcNAc2, MALDI-TOF mass spectrometry, overview
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?
additional information
?
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polyglycine hydrolases cleave peptide bonds in the polyglycine interdomain linker of the enzyme producing a 24 kDa chitinase protein from the C-terminus and several small, cysteine-rich chitin-binding peptides derived from the N-terminus
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?
additional information
?
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enzyme activity on the modified chitopentaose substrate GlcN-GlcNAc4, which contains a non-acetylated glucosamine residue at the nonreducing end, and on the modified chitotetraose substrate TMG-chitotriomycin-pMP, which contains a N-trimethylamino glucosamine (TMG) residue at the non-reducing end, and a p-methyoxyphenyl protecting group at the anomeric position, cleavage product is TMG-GlcNAc2, MALDI-TOF mass spectrometry, overview
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