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Literature summary for 3.2.1.14 extracted from

  • Watanabe, T.; Kanai, R.; Kawase, T.; Tanabe, T.; Mitsutomi, M.; Sakuda, S.; Miyashita, K.
    Family 19 chitinases of Streptomyces species: characterization and distribution (1999), Microbiology, 145, 3353-3363.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Serratia marcescens
-
Streptomyces griseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans WL12
-
-
-
Serratia marcescens
-
strains 2170 and QMB1466
-
Streptomyces atrovirens
-
-
-
Streptomyces coelicolor
-
-
-
Streptomyces cyanocolor
-
-
-
Streptomyces eurythermus
-
-
-
Streptomyces griseus
-
-
-
Streptomyces griseus HUT6037
-
-
-
Streptomyces ipomoeae
-
-
-
Streptomyces lividans
-
-
-
Streptomyces plicatus
-
-
-
Streptomyces prasinopilosus
-
-
-
Streptomyces scabiei
-
-
-
Streptomyces sp.
-
strains S15, S84, S100, S159
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces griseus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
-
Niallia circulans
0.16
-
powdered prawn shell chitin Streptomyces griseus
0.19
-
squid chitin Streptomyces griseus
0.2
-
chitosan, 100% deacetylated chitin Streptomyces griseus
0.68
-
chitosan, 90% deacetylated chitin Streptomyces griseus
1.84
-
regenerated chitin Streptomyces griseus
8.06
-
chitosan, 80% deacetylated chitin Streptomyces griseus
9.73
-
chitosan, 70% deacetylated chitin Streptomyces griseus
24.5
-
colloidal chitin Streptomyces griseus
255
-
glycol chitin Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
Serratia marcescens N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitin + H2O
-
Niallia circulans N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitin + H2O
-
Streptomyces sp. N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitin + H2O colloidal and regenerated chitin, chitin from suqid and powdered prawn shell Streptomyces griseus N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitin + H2O
-
Niallia circulans WL12 N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitin + H2O colloidal and regenerated chitin, chitin from suqid and powdered prawn shell Streptomyces griseus HUT6037 N,N-diacetylchitobiose + N-acetylglucosamine
-
?
chitosan + H2O chitosan with different degrees of deacetylation Serratia marcescens ?
-
?
chitosan + H2O chitosan with different degrees of deacetylation Streptomyces griseus ?
-
?
chitosan + H2O chitosan with different degrees of deacetylation Streptomyces sp. ?
-
?
chitosan + H2O chitosan with different degrees of deacetylation Streptomyces griseus HUT6037 ?
-
?
glycol chitin + H2O
-
Niallia circulans N-acetylglucosamine + ?
-
?
glycol chitin + H2O
-
Streptomyces griseus N-acetylglucosamine + ?
-
?
glycol chitin + H2O
-
Niallia circulans WL12 N-acetylglucosamine + ?
-
?
glycol chitin + H2O
-
Streptomyces griseus HUT6037 N-acetylglucosamine + ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Streptomyces griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Streptomyces griseus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
-
Streptomyces griseus

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5
-
Streptomyces griseus