Literature summary for 3.2.1.14 extracted from

Gan, Z.; Yang, J.; Tao, N.; Lou, Z.; Mi, Q.; Meng, Z.; Zhang, K.Q.
Crystallization and preliminary crystallographic analysis of a chitinase from Clonostachys rosea (2009), Acta Crystallogr. Sect. F, 65, 386-388.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of CrChi1 in Escherichia coli strain BL21 (DE3)	Clonostachys rosea

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged CrChi1, hanging drop vapour diffusion method, 0.0005 ml of 20 mg/ml protein in 20 mM Tris-HCl buffer pH 8.0 is mixed with an equal volume of precipitant solution containing containing 0.2 M ammonium dihydrogen phosphate and 20% w/v PEG 3350, pH 4.6., equilibration against 0.2 ml of reservoir solution at 18°C, X-ray diffraction structure determination and analysis at 1.8 A resolution	Clonostachys rosea

Crystallization (Comment)

Organism

purified recombinant His-tagged CrChi1, hanging drop vapour diffusion method, 0.0005 ml of 20 mg/ml protein in 20 mM Tris-HCl buffer pH 8.0 is mixed with an equal volume of precipitant solution containing containing 0.2 M ammonium dihydrogen phosphate and 20% w/v PEG 3350, pH 4.6., equilibration against 0.2 ml of reservoir solution at 18°C, X-ray diffraction structure determination and analysis at 1.8 A resolution

Clonostachys rosea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, recombinant His-tagged enzyme, SDS-PAGE	Clonostachys rosea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Clonostachys rosea	chitinase Chi1 plays a role in the infection of nematodes by the fungus	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Clonostachys rosea	-	a nematophagous fungus	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged CrChi1 from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography	Clonostachys rosea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	chitinase Chi1 plays a role in the infection of nematodes by the fungus	Clonostachys rosea	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000, recombinant His-tagged enzyme, SDS-PAGE	Clonostachys rosea

Synonyms

Synonyms	Comment	Organism
Chi1	-	Clonostachys rosea