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Literature summary for 3.2.1.14 extracted from

  • Kuo, C.J.; Liao, Y.C.; Yang, J.H.; Huang, L.C.; Chang, C.T.; Sung, H.Y.
    Cloning and characterization of an antifungal class III chitinase from suspension-cultured bamboo (Bambusa oldhamii) cells (2008), J. Agric. Food Chem., 56, 11507-11514.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Chi3-1, expression in Pichia pastoris strain X-33 Bambusa oldhamii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28300
-
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE Bambusa oldhamii
35700
-
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE Bambusa oldhamii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Bambusa oldhamii
-
?
-
?
additional information Bambusa oldhamii recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum ?
-
?

Organism

Organism UniProt Comment Textmining
Bambusa oldhamii
-
gene Chi3-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Bambusa oldhamii

Purification (Commentary)

Purification (Comment) Organism
recombinant Chi3 from Pichia pastoris strain X-33 by sequential ammonium sulfate fractionation, hydrophobic interaction chromatography, and Con A affinity chromatography to homogeneity Bambusa oldhamii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
117
-
purified recombinant nonglycosylated Chi3 Bambusa oldhamii
289
-
purified recombinant glycosylated Chi3 Bambusa oldhamii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
Bambusa oldhamii ?
-
?
chitin + H2O cleavage product analysis, overview Bambusa oldhamii ?
-
?
ethylene glycol chitin + H2O
-
Bambusa oldhamii ?
-
?
additional information recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum Bambusa oldhamii ?
-
?
additional information Chi3 acts as an endohydrolase, it cleaves chitin trimers into monomers and dimers and cleaves chitin tetramer to hexamer into a mixture of monomers, dimers, and trimers Bambusa oldhamii ?
-
?

Subunits

Subunits Comment Organism
? x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE Bambusa oldhamii

Synonyms

Synonyms Comment Organism
class III chitinase
-
Bambusa oldhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
nonglycosylated enzyme Chi3 Bambusa oldhamii
80
-
glycosylated enzyme Chi3 Bambusa oldhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
glycosylated enzyme Chi3 Bambusa oldhamii
4
-
nonglycosylated enzyme Chi3 Bambusa oldhamii