Cloned (Comment) | Organism |
---|---|
gene Chi3-1, expression in Pichia pastoris strain X-33 | Bambusa oldhamii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28300 | - |
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
35700 | - |
x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Bambusa oldhamii | - |
? | - |
? | |
additional information | Bambusa oldhamii | recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bambusa oldhamii | - |
gene Chi3-1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bambusa oldhamii |
Purification (Comment) | Organism |
---|---|
recombinant Chi3 from Pichia pastoris strain X-33 by sequential ammonium sulfate fractionation, hydrophobic interaction chromatography, and Con A affinity chromatography to homogeneity | Bambusa oldhamii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
117 | - |
purified recombinant nonglycosylated Chi3 | Bambusa oldhamii |
289 | - |
purified recombinant glycosylated Chi3 | Bambusa oldhamii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | - |
Bambusa oldhamii | ? | - |
? | |
chitin + H2O | cleavage product analysis, overview | Bambusa oldhamii | ? | - |
? | |
ethylene glycol chitin + H2O | - |
Bambusa oldhamii | ? | - |
? | |
additional information | recombinant glycosylated and nonglycosylated BoCHI3-1 isoforms show antifungal activity against Scolecobasidium longiphorum | Bambusa oldhamii | ? | - |
? | |
additional information | Chi3 acts as an endohydrolase, it cleaves chitin trimers into monomers and dimers and cleaves chitin tetramer to hexamer into a mixture of monomers, dimers, and trimers | Bambusa oldhamii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35700, glycosylated Chi3, SDS-PAGE, x * 28300, nonglycosylated Chi3, SDS-PAGE | Bambusa oldhamii |
Synonyms | Comment | Organism |
---|---|---|
class III chitinase | - |
Bambusa oldhamii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
nonglycosylated enzyme Chi3 | Bambusa oldhamii |
80 | - |
glycosylated enzyme Chi3 | Bambusa oldhamii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
glycosylated enzyme Chi3 | Bambusa oldhamii |
4 | - |
nonglycosylated enzyme Chi3 | Bambusa oldhamii |