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Literature summary for 3.2.1.14 extracted from

  • Songsiriritthigul, C.; Lapboonrueng, S.; Pechsrichuang, P.; Pesatcha, P.; Yamabhai, M.
    Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste (2010), Biores. Technol., 101, 4096-4103.
    View publication on PubMed
Search for more literature for 3.2.1.14

Application

Application Comment Organism
synthesis recombinant enzyme produced in Escherichia coli can efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50°C and retains its activity up to 3 days under these conditions Bacillus licheniformis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside pH 6.0, 37°C Bacillus licheniformis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bacillus licheniformis 5829
-
periplasm
-
 Bacillus licheniformis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66801, calculated, x * 66000, SDS-PAGE Bacillus licheniformis
66801
-
 x * 66801, calculated, x * 66000, SDS-PAGE Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis B7UB89 isoform ChiA; strain DSM8785
-
Bacillus licheniformis DSM 8785 B7UB89 isoform ChiA; strain DSM8785
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.294
-
 substrate 4-nitrophenyl-chitobiose, pH 6.0, 37°C Bacillus licheniformis

Storage Stability

Storage Stability Organism
-25°C, 20% glycerol, up to one year with more than 80% residual activity Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis DSM 8785 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
colloidal chitin + H2O
-
 Bacillus licheniformis N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
colloidal chitin + H2O
-
 Bacillus licheniformis DSM 8785 N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 66801, calculated, x * 66000, SDS-PAGE Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside pH 6.0, 37°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11
-
 Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.3
-
 4-nitrophenyl-chitobiose pH 6.0, 37°C Bacillus licheniformis