Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Bacillus thuringiensis |
Cloned (Comment) | Organism |
---|---|
gene chiA, expression in Escherichia coli strain XL-1 Blue | Bacillus thuringiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibits 57.2% at 10 mM | Bacillus thuringiensis | |
Cr3+ | inhibits 12.3% at 10 mM | Bacillus thuringiensis | |
Mn2+ | inhibits 7.6% at 10 mM | Bacillus thuringiensis | |
SDS | inhibits 9.1% at 10 mM | Bacillus thuringiensis | |
Zn2+ | inhibits 63.8% at 10 mM | Bacillus thuringiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates 15% at 10 mM | Bacillus thuringiensis | |
Fe3+ | activates 6% at 10 mM | Bacillus thuringiensis | |
Mg2+ | activates 6% at 10 mM | Bacillus thuringiensis | |
Na+ | activates 6% at 10 mM | Bacillus thuringiensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, ChiA, SDS-PAGE | Bacillus thuringiensis |
72000 | - |
native PAGE | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
subsp. colmeri | - |
Bacillus thuringiensis 15A3 | - |
subsp. colmeri | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme by gel filtration | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin | - |
Bacillus thuringiensis | ? | - |
? | |
colloidal chitin | - |
Bacillus thuringiensis 15A3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, ChiA, SDS-PAGE | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
ChiA | - |
Bacillus thuringiensis |
chitinase A | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus thuringiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range | Bacillus thuringiensis |
General Information | Comment | Organism |
---|---|---|
physiological function | ChiA significantly inhibits the spore germination of four species of fungi. The IC50 of ChiA on the spore germination of Penicillium glaucum and Sclerotinia fuckelian are 0.0113 mg/ml and 0.0106 mg/ml, respectively. The crude ChiA protein at 12.6 mU reduced the LC50 of the crystal protein of strain 15A3 against the larvae of Spodoptera exigua and Helicoverpa armigera | Bacillus thuringiensis |