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Literature summary for 3.2.1.14 extracted from

  • Liu, D.; Cai, J.; Xie, C.; Liu, C.; Chen, Y.
    Purification and partial characterization of a 36-kDa chitinase from Bacillus thuringiensis subsp. colmeri, and its biocontrol potential (2010), Enzyme Microb. Technol., 46, 252-256.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
Bacillus thuringiensis

Cloned(Commentary)

Cloned (Comment) Organism
gene chiA, expression in Escherichia coli strain XL-1 Blue Bacillus thuringiensis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inhibits 57.2% at 10 mM Bacillus thuringiensis
Cr3+ inhibits 12.3% at 10 mM Bacillus thuringiensis
Mn2+ inhibits 7.6% at 10 mM Bacillus thuringiensis
SDS inhibits 9.1% at 10 mM Bacillus thuringiensis
Zn2+ inhibits 63.8% at 10 mM Bacillus thuringiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activates 15% at 10 mM Bacillus thuringiensis
Fe3+ activates 6% at 10 mM Bacillus thuringiensis
Mg2+ activates 6% at 10 mM Bacillus thuringiensis
Na+ activates 6% at 10 mM Bacillus thuringiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
2 * 36000, ChiA, SDS-PAGE Bacillus thuringiensis
72000
-
native PAGE Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
subsp. colmeri
-
Bacillus thuringiensis 15A3
-
subsp. colmeri
-

Purification (Commentary)

Purification (Comment) Organism
extracellular enzyme by gel filtration Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin
-
Bacillus thuringiensis ?
-
?
colloidal chitin
-
Bacillus thuringiensis 15A3 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, ChiA, SDS-PAGE Bacillus thuringiensis

Synonyms

Synonyms Comment Organism
ChiA
-
Bacillus thuringiensis
chitinase A
-
Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus thuringiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Bacillus thuringiensis

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range Bacillus thuringiensis

General Information

General Information Comment Organism
physiological function ChiA significantly inhibits the spore germination of four species of fungi. The IC50 of ChiA on the spore germination of Penicillium glaucum and Sclerotinia fuckelian are 0.0113 mg/ml and 0.0106 mg/ml, respectively. The crude ChiA protein at 12.6 mU reduced the LC50 of the crystal protein of strain 15A3 against the larvae of Spodoptera exigua and Helicoverpa armigera Bacillus thuringiensis