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Literature summary for 3.2.1.14 extracted from

  • Songsiriritthigul, C.; Pesatcha, P.; Eijsink, V.G.; Yamabhai, M.
    Directed evolution of a Bacillus chitinase (2009), Biotechnol. J., 4, 501-509.
    View publication on PubMed
Search for more literature for 3.2.1.14

Protein Variants

Protein Variants Comment Organism
E157G/S193D/A216V/P565S complete loss of activity Bacillus licheniformis
N29S/A234V/Q387R/V465A/D480N kcat/Km of the mutant chitinase is 2.7- and 2.3fold higher than the average kcat/Km of wild types at pH 3.0 and 6.0 Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside mutant N29S/A234V/Q387R/V465A/D480N, pH 6.0, 37°C Bacillus licheniformis
0.03
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM8785, pH 6.0, 37°C Bacillus licheniformis
0.06
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside mutant N29S/A234V/Q387R/V465A/D480N, pH 3.0, 37°C Bacillus licheniformis
0.06
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM13, pH 6.0, 37°C Bacillus licheniformis
0.1
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM13, pH 3.0, 37°C Bacillus licheniformis
0.11
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM8785, pH 3.0, 37°C Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis B7UB89 isoform ChiA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 60
-
 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM8785, pH 3.0, 37°C Bacillus licheniformis
0.17
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM13, pH 3.0, 37°C Bacillus licheniformis
0.23
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside mutant N29S/A234V/Q387R/V465A/D480N, pH 3.0, 37°C Bacillus licheniformis
0.31
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM8785, pH 6.0, 37°C Bacillus licheniformis
0.43
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside wild-type, strain DSM13, pH 6.0, 37°C Bacillus licheniformis
0.65
-
 4-nitrophenyl beta-N,N'-diacetylchitobioside mutant N29S/A234V/Q387R/V465A/D480N, pH 6.0, 37°C Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.36
-
 4-nitrophenyl-chitobiose wild-type, strain DSM8785, pH 3.0, 37°C Bacillus licheniformis
1.69
-
 4-nitrophenyl-chitobiose wild-type, strain DSM13, pH 3.0, 37°C Bacillus licheniformis
4.1
-
 4-nitrophenyl-chitobiose mutant N29S/A234V/Q387R/V465A/D480N, pH 3.0, 37°C Bacillus licheniformis
7.55
-
 4-nitrophenyl-chitobiose wild-type, strain DSM13, pH 6.0, 37°C Bacillus licheniformis
10.3
-
 4-nitrophenyl-chitobiose wild-type, strain DSM8785, pH 6.0, 37°C Bacillus licheniformis
19.4
-
 4-nitrophenyl-chitobiose mutant N29S/A234V/Q387R/V465A/D480N, pH 6.0, 37°C Bacillus licheniformis