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Literature summary for 3.2.1.14 extracted from
- Biochemical characterization of chitinase 2 expressed during the autolytic phase of the inky cap, Coprinellus congregatus (2013), J. Microbiol., 51, 189-193.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chi2, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115 | Coprinellus congregatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | pH 4.0, 35°C, recombinant enzyme | Coprinellus congregatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, recombinant enzyme | Coprinellus congregatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinellus congregatus | H9CWQ0 | gene chi2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the enzyme is expressed most at the mature mushroom stage when the mushroom tissues entered the autolytic process, no expression in hyphae | Coprinellus congregatus | - |
| primordium | lower expression level | Coprinellus congregatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Coprinellus congregatus | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, recombinant enzyme | Coprinellus congregatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHI2 | - |
Coprinellus congregatus |
| Chi2 enzyme | - |
Coprinellus congregatus |
| chitinase 2 | - |
Coprinellus congregatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
recombinant enzyme | Coprinellus congregatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 45 | activity range, profile overview | Coprinellus congregatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
broad optimum, recombinant enzyme | Coprinellus congregatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range with high activity, profile overview | Coprinellus congregatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | chitinase 2 ia a chitinase family 18 enzyme | Coprinellus congregatus |
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Release 2023.1 (January 2023)
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