Cloned (Comment) | Organism |
---|---|
gene Cht-2, expression in Escherichia coli | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Oryza sativa | chitinase is a glycoside hydrolase that catalyzes the degradation of chitin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
ssp. japonica, cv. Nipponbare, gene Cht-2 | - |
Purification (Comment) | Organism |
---|---|
recombinant OsChia1b from Escherichia coli by ammonium sulfate fractionation and chitin affinity chromatography to homogeneity | Oryza sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
225 | - |
purified OsChia1b, substrate colloidal chitin | Oryza sativa |
253 | - |
purified OsChia1b, substrate glycol chitin | Oryza sativa |
254 | - |
purified OsChia1b, substrate GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc | Oryza sativa |
2211 | - |
purified OsChia1b, substrate WS-chitin | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | chitinase is a glycoside hydrolase that catalyzes the degradation of chitin | Oryza sativa | ? | - |
? | |
chitin + H2O | WS-chitin and colloidal chitin substrate | Oryza sativa | ? | - |
? | |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Oryza sativa | ? | - |
? | |
glycol chitin + H2O | - |
Oryza sativa | ? | - |
? | |
additional information | OsChia1b shows no activity against N-acetylchitotriose, and crystalline beta-chitin is a poor substrate. OsChia1b substrate binding specificity depends on the N-acetyl groups of the substrate. About 80% of added OsChia1b binds to colloidal chitin, whereas less than 30% of added OsChia1b binds to chitosan 7B, with 70% deacetylated chitin, avicel, and acid-swollen cellulose, less than 10% of OsChia1b binds to beta-1,3-glucan, curdlan | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class I chitinase | - |
Oryza sativa |
More | OsChia1b is a class I chitinase of glucohydrolase family 19 | Oryza sativa |
OsChia1b | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Oryza sativa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | - |
Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Oryza sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 12 | - |
Oryza sativa |