Information on EC 3.2.1.14 - chitinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.14
-
RECOMMENDED NAME
GeneOntology No.
chitinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
chitin degradation I (archaea)
-
-
chitin degradation II (Vibrio)
-
-
chitin degradation III (Serratia)
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase
Some chitinases also display the activity defined in EC 3.2.1.17 lysozyme.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-06-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain D1
-
-
Manually annotated by BRENDA team
strain D1
-
-
Manually annotated by BRENDA team
strain SUWA-9
SwissProt
Manually annotated by BRENDA team
10S-24
-
-
Manually annotated by BRENDA team
10S-24
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain O-7
-
-
Manually annotated by BRENDA team
strain O-7
-
-
Manually annotated by BRENDA team
ectomycorrhizal fungus, strain 16-3
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain KF8, isolated from rumen fluid of fistulated cow
-
-
Manually annotated by BRENDA team
strain KF8, isolated from rumen fluid of fistulated cow
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain A26, gene chiA
UniProt
Manually annotated by BRENDA team
strain A26, gene chiA
UniProt
Manually annotated by BRENDA team
S1-13
-
-
Manually annotated by BRENDA team
S1-13
-
-
Manually annotated by BRENDA team
cv. Adamo and Markant
UniProt
Manually annotated by BRENDA team
strain 28-9
-
-
Manually annotated by BRENDA team
NCTU2
-
-
Manually annotated by BRENDA team
strain TKU006, isolated from the soil in Taiwan
-
-
Manually annotated by BRENDA team
isolated from Tamsui mangrove river sediment in Taiwan
-
-
Manually annotated by BRENDA team
strain KA-304
-
-
Manually annotated by BRENDA team
No. 4.1
-
-
Manually annotated by BRENDA team
strain WL-12
-
-
Manually annotated by BRENDA team
strain WL12
-
-
Manually annotated by BRENDA team
gene CHI
UniProt
Manually annotated by BRENDA team
strain DSM13
-
-
Manually annotated by BRENDA team
isoform ChiA; strain DSM8785
UniProt
Manually annotated by BRENDA team
strain MY75
-
-
Manually annotated by BRENDA team
strain SK-1, isolated from soil in Angthong Province, Thailand
-
-
Manually annotated by BRENDA team
isoform ChiS; strain SG2
UniProt
Manually annotated by BRENDA team
isoform ChiS; strain SG2
UniProt
Manually annotated by BRENDA team
BG-11
-
-
Manually annotated by BRENDA team
isolated from a soil sample collected from hot spring of XinJiang Province, China
-
-
Manually annotated by BRENDA team
strain MH-1
-
-
Manually annotated by BRENDA team
NCTU2
-
-
Manually annotated by BRENDA team
isolated from Korean traditional soy sauce
UniProt
Manually annotated by BRENDA team
Wy22
-
-
Manually annotated by BRENDA team
isolated from natto, a traditional Japanese food, 3 isozymes Chi1-3
-
-
Manually annotated by BRENDA team
isolated from leaves and root rhizospheres of plants in Turkey
-
-
Manually annotated by BRENDA team
subsp. colmeri
-
-
Manually annotated by BRENDA team
strain WB7
-
-
Manually annotated by BRENDA team
gene Chi3-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from the Great Indian Desert soils
-
-
Manually annotated by BRENDA team
isolated from the Great Indian Desert soils
-
-
Manually annotated by BRENDA team
cv. Manchar, cold-responsive chitinase gene BiCHT1
UniProt
Manually annotated by BRENDA team
parasitic nematode in Onchocerca volvulus larvae
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
KH2
-
-
Manually annotated by BRENDA team
Calvatia cyanthiformis
puffball
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
diverse strains, isolated from environmental sources, water or a Sphagnum pond, overview
-
-
Manually annotated by BRENDA team
diverse strains, isolated from environmental sources, water or a Sphagnum pond, overview
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 191
-
-
Manually annotated by BRENDA team
gene chi46
UniProt
Manually annotated by BRENDA team
near curvimaculatus, endoparasitic wasp
-
-
Manually annotated by BRENDA team
isozymes ChiA, ChiB, and ChiC
-
-
Manually annotated by BRENDA team
strain C-61
SwissProt
Manually annotated by BRENDA team
gene chi54
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
str. nov.
-
-
Manually annotated by BRENDA team
str. nov.
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain M-21
SwissProt
Manually annotated by BRENDA team
strain M-21
SwissProt
Manually annotated by BRENDA team
isolated from the intestinal tract of Otaria flavescens
-
-
Manually annotated by BRENDA team
gene chi2
UniProt
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
gene SafchiA
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. Galia and Bredor
-
-
Manually annotated by BRENDA team
pumpkin
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. Flakkese SG766 Trophy
-
-
Manually annotated by BRENDA team
diversity of the species
-
-
Manually annotated by BRENDA team
isozyme analysis, diversity of the species
-
-
Manually annotated by BRENDA team
strain NRG4
-
-
Manually annotated by BRENDA team
strain NRG4
-
-
Manually annotated by BRENDA team
jelly fig
-
-
Manually annotated by BRENDA team
several strains, genes chiA, chiB, chiC, and chiD
-
-
Manually annotated by BRENDA team
different strins, genes chiA, chiB, chiC, and chiD
-
-
Manually annotated by BRENDA team
variety topaz
-
-
Manually annotated by BRENDA team
arbiscular mycorrhizal fungus inoculated in roots of grapevine, Vitis amurensis, gene VCH3
-
-
Manually annotated by BRENDA team
hard tick, strain Okayama
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB
UniProt
Manually annotated by BRENDA team
DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB
UniProt
Manually annotated by BRENDA team
ectomycorrhizal fungus, strain 9-12 and 9-1
-
-
Manually annotated by BRENDA team
isolated from subseafloor core sediment at 0.5 mbsf
-
-
Manually annotated by BRENDA team
isolated from subseafloor core sediment at 0.5 mbsf
-
-
Manually annotated by BRENDA team
ssp. lactis, strain IL1403, a derivative of the strain IL594 that is isolated from a cheese starter culture, gene LlChi18A
UniProt
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain Div-A8; strains Clip 11262 and Div-A8
-
-
Manually annotated by BRENDA team
strain Div-A8
-
-
Manually annotated by BRENDA team
diverse strains and serotypes, overview. Strain EGD, two isozymes lmo0105 and lmo1883, encoded by genes chiB and chiA, respectively
-
-
Manually annotated by BRENDA team
LMG 11386
-
-
Manually annotated by BRENDA team
LMG 11386
-
-
Manually annotated by BRENDA team
strain LMG 11389
-
-
Manually annotated by BRENDA team
strain LMG 11389
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
isolated from chitin rich soil of Andhra Pradesh, India
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
V2
-
-
Manually annotated by BRENDA team
V2
-
-
Manually annotated by BRENDA team
CCRC31499
-
-
Manually annotated by BRENDA team
CCRC31499
-
-
Manually annotated by BRENDA team
i.e. Vibrio marinus, gene chi60
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
i.e. Mythimna separata
SwissProt
Manually annotated by BRENDA team
no activity in Listeria grayi
strain LMG 16491
-
-
Manually annotated by BRENDA team
no activity in Listeria grayi LMG 16491
strain LMG 16491
-
-
Manually annotated by BRENDA team
strain OPC-131, gene chiB337
-
-
Manually annotated by BRENDA team
strain OPC-131, gene chiB337
-
-
Manually annotated by BRENDA team
strain F96
-
-
Manually annotated by BRENDA team
strain F96
-
-
Manually annotated by BRENDA team
Orpinomyces sp.
strain KF7, isolated from rumen fluid of fistulated cow
-
-
Manually annotated by BRENDA team
strain KF7, isolated from rumen fluid of fistulated cow
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
produces several different chitinases
UniProt
Manually annotated by BRENDA team
isolated from compost (55°C) in Japan
-
-
Manually annotated by BRENDA team
isolated from compost (55°C) in Japan
-
-
Manually annotated by BRENDA team
C.A. Meyer
UniProt
Manually annotated by BRENDA team
strain Pb01, ATCC MYA 826, gene cts1
SwissProt
Manually annotated by BRENDA team
strain Pb01, ATCC MYA 826, gene cts1
SwissProt
Manually annotated by BRENDA team
strain LYG 0704
-
-
Manually annotated by BRENDA team
strain LYG 0704
-
-
Manually annotated by BRENDA team
silver croaker fish
-
-
Manually annotated by BRENDA team
parsley, treated with fungal elicitor
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
pokeweed
-
-
Manually annotated by BRENDA team
isoform ChiA4
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
hybrid H11-11, gene win6
-
-
Manually annotated by BRENDA team
gene Ptchi19
-
-
Manually annotated by BRENDA team
strain GRC3
-
-
Manually annotated by BRENDA team
isolated from soil in Taiwan
-
-
Manually annotated by BRENDA team
strain TKU015
-
-
Manually annotated by BRENDA team
two class III isozymes
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Salmonella enterica typhimurium
gene SL0018 or chiA
-
-
Manually annotated by BRENDA team
Salmonella enterica typhimurium SL1344
gene SL0018 or chiA
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
reptilia: Phrynosomatidae
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
chitinase- and chitobiase-overproducing mutant SJ101
-
-
Manually annotated by BRENDA team
strain TU09
UniProt
Manually annotated by BRENDA team
strain TU09
UniProt
Manually annotated by BRENDA team
tomato
-
-
Manually annotated by BRENDA team
isolated from the rhizosphere of winter wheat
-
-
Manually annotated by BRENDA team
isolated from the rhizosphere of winter wheat
-
-
Manually annotated by BRENDA team
strain SU-1, two extracellular isozymes
-
-
Manually annotated by BRENDA team
strain SU-1, two extracellular isozymes
-
-
Manually annotated by BRENDA team
WYEC108
-
-
Manually annotated by BRENDA team
strain 2B
-
-
Manually annotated by BRENDA team
fragment; marine strain
UniProt
Manually annotated by BRENDA team
strain J-13-3
-
-
Manually annotated by BRENDA team
M-20
-
-
Manually annotated by BRENDA team
RC 1071
-
-
Manually annotated by BRENDA team
strain P10
-
-
Manually annotated by BRENDA team
strain P10
-
-
Manually annotated by BRENDA team
ectomycorrhizal fungus, strain 15-4, no activity in strain 15-3
-
-
Manually annotated by BRENDA team
ectomycorrhizal fungus, strain 14-7
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
var. levisporus
UniProt
Manually annotated by BRENDA team
strain PC-3-7, gene chi46
-
-
Manually annotated by BRENDA team
strain PC-3-7, gene chi46
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
strain TK-22
-
-
Manually annotated by BRENDA team
precursor protein; strain No. 442
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. Astar and cv. Piestansky
-
-
Manually annotated by BRENDA team
gene VvChi4D
UniProt
Manually annotated by BRENDA team
plant pathogen, strains 9713 and Temecula in Vinis sp., and strains 9a5c, SR3, and SR28 in Citrus sp., secreted serine- and metallo-protease with chitinase activity
-
-
Manually annotated by BRENDA team
gene YlCTS1
-
-
Manually annotated by BRENDA team
gene CHIA
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(GlcNAc)6 + H2O
(GlcNAc)2 + (GlcNAc)3 + (GlcNAc)4
show the reaction diagram
the enzyme prefers the beta-anomer of chito-oligosaccharides as substrate, cleavage of beta-glycosidic linkages from the non-reducing end primarily by disaccharide units
product analysis
-
r
(GlcNAc)7 + H2O
?
show the reaction diagram
-
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-acetamido-2-deoxy-4-O-methyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-acetamido-4-O-cyclohexylmethyl-2-deoxy-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
2-acetamido-2-deoxy-4-O-(2-deoxy-2-acetamido-4-O-isopropyl-beta-D-glucopyranosyl)-1-O-4-methylumbelliferyl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
3,4-dinitrophenyltetra-N-acetyl-beta-D-chitotetraoside + H2O
?
show the reaction diagram
4'''-O-(4-methylumbelliferyl)-chitotetraose + H2O
4-O-(4-methylumbelliferyl)-N-acetyl-D-glucosamine + chitotriose
show the reaction diagram
-
-
-
-
?
4'-O-(4-methylumbelliferyl)-chitobiose + H2O
4-O-(4-methylumbelliferyl)-N-acetyl-D-glucosamine + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-triacetylchitotriose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferone + beta-D-N,N',N''-triacetylchitotriose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
4-methylumbelliferol + beta-N,N',N''-triacetylchitotriose
show the reaction diagram
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-N,N'-diacetylchitobioside
?
show the reaction diagram
-
-
-
-
-
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
4-methylumbelliferol + beta-N,N'-diacetylchitobiose
show the reaction diagram
4-methylumbelliferyl beta-N-acetyl-D-glucosamine + H2O
4-methylumbelliferol + beta-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
4-methylumbelliferyl chitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl chitobioside + H2O
4-methylumbelliferol + chitobiose
show the reaction diagram
4-methylumbelliferyl chitotriose + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-methylumbelliferone + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
4-methylumbelliferyl N,N',N''-beta-D-triacetylchitotrioside + H2O
4-methylumbelliferol + N,N',N''-beta-D-triacetylchitotriose
show the reaction diagram
substrate is prefered over shorter GlcNAc-substrates
-
-
?
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
4-methylumbelliferone + N,N',N''-triacetylchitotriose
show the reaction diagram
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O
?
show the reaction diagram
4-methylumbelliferyl N,N'-diacetylchitotriose + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl tri-N-acetylchitotriose
?
show the reaction diagram
-
-
-
-
-
4-methylumbelliferyl-(4-deoxy)chitobiose + H2O
4-methylumbelliferol + (4-deoxy)chitobiose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-N, N'-diacetylchitobiose + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-N,N',N''-triacetyl-chitotriose + H2O
4-methylumbelliferol + beta-D-N,N',N''-triacetyl-chitotriose
show the reaction diagram
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotriose + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O
?
show the reaction diagram
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O
?
show the reaction diagram
4-methylumbelliferyl-D-N,N'-diacetylchito-bioside + H2O
4-methylumbelliferone + D-N,N'-diacetylchito-bioside
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-deoxychitobioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O
?
show the reaction diagram
4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
?
show the reaction diagram
4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
?
show the reaction diagram
4-methylumbelliferyl-N,N'N''-triacetyl-beta-chitotrioside + H2O
? + 4-methylumbelliferone
show the reaction diagram
4-methylumbelliferyl-tri-N-acetylchitotriose + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-GlcNAc + H2O
4-nitrophenol + N-acetyl-D-glucosamine
show the reaction diagram
-
0.8% of the activity with 4-nitrophenyl-(beta-D-GlcNAc)3
-
-
?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
?
show the reaction diagram
4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
4-nitrophenol + beta-N,N',N'',N'''-tetraacetylchitotetraose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + beta-N,N',N''-triacetylchitotriose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
4-nitrophenol + N,N',N''-triacetylchitotriose
show the reaction diagram
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
4-nitrophenol + beta-N,N'-diacetylchitobiose
show the reaction diagram
-
-
-
?
4-nitrophenyl chitobiose + H2O
4-nitrophenol + chitobiose
show the reaction diagram
4-nitrophenyl chitobiose + H2O
?
show the reaction diagram
4-nitrophenyl chitobioside + H2O
4-nitrophenol + chitobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl chitotetraose + H2O
4-nitrophenol + chitobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl chitotriose + H2O
?
show the reaction diagram
4-nitrophenyl di-N,N'-acetyl-beta-D-chitobiose + H2O
?
show the reaction diagram
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
show the reaction diagram
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
?
show the reaction diagram
4-nitrophenyl N,N'-diacetylchitobiose + H2O
4-nitrophenol + N,N'-diacetylchitobiose
show the reaction diagram
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
show the reaction diagram
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
p-nitrophenol + N-acetyl-D-glucosamine
show the reaction diagram
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
show the reaction diagram
4-nitrophenyl N-acetyl-D-glucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
show the reaction diagram
4-nitrophenyl N-acetylchitobioside + H2O
4-nitrophenol + N-acetylchitobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl N-acetylchitohexaoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl N-acetylchitopentaoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl N-acetylchitotetraoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl N-acetylchitotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl tri-N-acetylchitotrioside + H2O
4-nitrophenol + tri-N-acetylchitotriose
show the reaction diagram
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose + H2O
4-nitrophenol + beta-1,4-N,N'-diacetyl-chitobiose
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-1,4-N,N'-diacetyl-chitobiose + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl-beta-N,N'-diacetylchitobioside + H2O
?
show the reaction diagram
essential role of the two carboxylic acid residues E172 and D170 in the catalytic mechanism of Chit42
-
-
?
4-nitrophenyl-chitobiose + H2O
4-nitrophenol + chitobiose
show the reaction diagram
4-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
4-nitrophenol + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
6-O-hydroxypropylchitin + H2O
?
show the reaction diagram
-
-
-
-
?
acetylchitine + H2O
?
show the reaction diagram
-
-
-
-
?
allyl penta-N-acetyl-chitopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-chitin + H2O
?
show the reaction diagram
alpha-crystal chitin + H2O
di-N-acetyl-chitobiose
show the reaction diagram
alpha-type chitin + H2O
?
show the reaction diagram
alpha-type chitosan + H2O
?
show the reaction diagram
-
98% deacetylated, 18% of the activity with colloidal chitin
-
-
?
ball-milled chitin + H2O
?
show the reaction diagram
-
-
-
-
?
beta-1,4-N-acetylglucosamine polymer + H2O
?
show the reaction diagram
beta-chitin + H2O
?
show the reaction diagram
beta-type chitin + H2O
?
show the reaction diagram
-
best substrate, 148% of the activity with colloidal chitin
-
-
?
beta-type chitosan + H2O
?
show the reaction diagram
-
98% deacetylated, 26% of the activity with colloidal chitin
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl chitin + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl-chitin + H2O
?
show the reaction diagram
carboxymethyl-Chitin-Remazol-Brilliant-Violet + H2O
?
show the reaction diagram
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
?
show the reaction diagram
carboxymethylated-chitin-remazol brilliant violet + H2O
?
show the reaction diagram
carboxymethylchitin-Remazol Brilliant Violet 5R + H2O
?
show the reaction diagram
-
-
-
?
cell wall + H2O
chitin oligosaccharides
show the reaction diagram
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
chitin + H2O
?
show the reaction diagram
chitin + H2O
chitobiose
show the reaction diagram
chitin + H2O
chitobiose + ?
show the reaction diagram
chitin + H2O
chitotriose
show the reaction diagram
chitin + H2O
GlcNAc + ?
show the reaction diagram
-
-
-
?
chitin + H2O
GlcNAc2
show the reaction diagram
chitin + H2O
N,N'-diacetylchitobiose
show the reaction diagram
chitin + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N-acetylglucosamine
show the reaction diagram
chitin + H2O
N,N-diacetylchitobiose + N-acetylD-glucosamine
show the reaction diagram
-
-
-
?
chitin + H2O
N,N-diacetylchitobiose + N-acetylglucosamine
show the reaction diagram
chitin + H2O
N-acetyl-D-glucosamine + ?
show the reaction diagram
chitin + H2O
N-acetylglucosamine + N-acetylchitobiose
show the reaction diagram
chitin azure + H2O
?
show the reaction diagram
-
-
-
-
?
chitin oligosaccharides + H2O
N-acetyl glucosamine
show the reaction diagram
glycol chitin assay method, (GlcNAc)1-6
-
-
?
chitin powder + H2O
?
show the reaction diagram
-
16.6% of the activity with colloidal chitin
-
-
?
chito-oligosaccharides + H2O
?
show the reaction diagram
-
-
-
?
chitobiose + H2O
2 D-glucosamine
show the reaction diagram
chitobiose + H2O
2 N-acetyl-beta-D-glucosamine
show the reaction diagram
chitohexaose + chitobiose
chitooctaose + H2O
show the reaction diagram
-
-
-
-
?
chitohexaose + H2O
2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose
show the reaction diagram
chitohexaose + H2O
?
show the reaction diagram
chitohexaose + H2O
chitobiose
show the reaction diagram
chitohexaose + H2O
chitobiose + chitotetraose + chitotriose
show the reaction diagram
chitohexaose + H2O
chitobiose + chitotriose
show the reaction diagram
chitohexaose + H2O
chitotetraose + chitobiose
show the reaction diagram
-
the enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)6
-
-
?
chitooligosaccharides + H2O
?
show the reaction diagram
chitopentaose + H2O
5 N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
?
chitopentaose + H2O
?
show the reaction diagram
chitopentaose + H2O
chitobiose
show the reaction diagram
-
-
major product
-
?
chitopentaose + H2O
chitobiose + chitotriose
show the reaction diagram
chitopentaose + H2O
chitobiose + chitotriose + D-glucosamine
show the reaction diagram
-
-
-
-
?
chitopentaose + H2O
chitobiose + D-glucosamine
show the reaction diagram
chitopentaose + H2O
chitotetraose + N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
-
?
chitopentaose + H2O
chitotriose + chitobiose + N-acetylglucosamine
show the reaction diagram
chitopentaose + H2O
N-acetylglucosamine + 2 N,N'-diacetylchitobiose
show the reaction diagram
reaction via trimer formation
-
-
?
chitosan
?
show the reaction diagram
-
-
-
-
?
chitosan + H2O
?
show the reaction diagram
chitosan + H2O
chitosan oligosaccharides
show the reaction diagram
chitosan + H2O
glucosamine + chitobiose + chitooligomers with a chain length longer than 6
show the reaction diagram
-
-
-
-
?
chitosan + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N'',N''',N''''-pentaacetylchitopentaose + N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + N,N',N'',N''',N'''',N''''',N''''''-heptaacetylchitoheptaose
show the reaction diagram
chitosan + H2O
oligosaccharides
show the reaction diagram
chitosan 7B + H2O
?
show the reaction diagram
chitotetraose + H2O
2 N-acetylglucosamine + N,N'-diacetylchitobiose
show the reaction diagram
reaction via trimer formation
-
-
?
chitotetraose + H2O
4 N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
?
chitotetraose + H2O
?
show the reaction diagram
-
-
-
?
chitotetraose + H2O
chitobiose
show the reaction diagram
chitotetraose + H2O
chitobiose + chitotriose + -D-glucosamine
show the reaction diagram
chitotetraose + H2O
chitobiose + chitotriose + D-glucosamine
show the reaction diagram
-
-
-
-
?
chitotetraose + H2O
N-acetylglucosamine + chitobiose + chitotriose
show the reaction diagram
chitotriose + H2O
3 N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
?
chitotriose + H2O
?
show the reaction diagram
chitotriose + H2O
chitobiose + D-glucosamine
show the reaction diagram
the rate of beta-(N-acetyl-beta-D-glucosamine)3 hydrolysis is higher than that of alpha-(N-acetyl-beta-D-glucosamine)3. Because enzyme Ra-ChiC is an inverting enzyme, chitotriose is supposed to bind predominantly to -2, -1, and +1 subsites
the products (N-acetyl-beta-D-glucosamine)2 and N-acetyl-beta-D-glucosamine are rich in alpha- and beta-forms, respectively
-
?
chitotriose + H2O
chitobiose + N-acetylglucosamine
show the reaction diagram
chitotriose + H2O
N-acetylglucosamine + chitobiose
show the reaction diagram
chitotriose + H2O
N-acetylglucosamine + N,N'-diacetylchitobiose
show the reaction diagram
-
-
-
?
CM-chitin-RBV + H2O
?
show the reaction diagram
colloid chitin + H2O
?
show the reaction diagram
colloidal chitin
?
show the reaction diagram
colloidal chitin + H2O
?
show the reaction diagram
colloidal chitin + H2O
chitobiose
show the reaction diagram
-
-
major product
-
?
colloidal chitin + H2O
chitobiose + chitotriose + chitotetraose
show the reaction diagram
colloidal chitin + H2O
chitobiose + D-glucosamine
show the reaction diagram
from crab shell
-
-
?
colloidal chitin + H2O
chitotriose + chitobiose + N-acetyl D-glucosamine
show the reaction diagram
-
-
-
-
?
colloidal chitin + H2O
GlcNAc + N,N'-diacetylchitobiose
show the reaction diagram
colloidal chitin + H2O
GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
colloidal chitin + H2O
N,N'-diacetylchitobiose
show the reaction diagram
-
the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity
-
?
colloidal chitin + H2O
N-acetyl-beta-D-glucosamine
show the reaction diagram
colloidal chitin + H2O
N-acetyl-D-glucosamine + ?
show the reaction diagram
-
-
-
-
?
colloidal chitin + H2O
N-acetyl-D-glucosamine + chitobiose
show the reaction diagram
colloidal chitin + H2O
N-acetylglucosamine + N-acetylchitobiose
show the reaction diagram
-
-
-
-
?
crab shell chitin + H2O
?
show the reaction diagram
the enzyme cleaves internal beta-1,4 glycosidic linkages in chitooligosaccharides randomly
-
-
?
crystalline alpha-chitin + H2O
GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)-GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
-
-
-
?
crystalline chitin + H2O
?
show the reaction diagram
deacetylated chitosan + H2O
?
show the reaction diagram
-
-
-
-
?
ethlene glycol chitin + H2O
?
show the reaction diagram
-
-
-
-
?
ethylene glycol chitin + H2O
?
show the reaction diagram
ethylene glycolchitin + H2O
?
show the reaction diagram
-
-
-
-
?
fluorescein isothiocyanate chitin + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta
show the reaction diagram
-
-
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNACbeta(1-4)GlcNAcbeta
show the reaction diagram
-
-
some (GlcNAc)3 is produced through transglycosylation reaction
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
i.e. (GlcNAc)6
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
show the reaction diagram
-
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
2 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
show the reaction diagram
-
i.e. (GlcNAc)6
-
-
?
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNACbeta(1-4)GlcNAcbeta
show the reaction diagram
-
-
product distribution is (GlcNAc)3 >(GlcNAc)4 > (GlcNAc)2 for the wild type enzyme. Some (GlcNAc)5 is produced, without formation of GlcNAc, suggesting that it is not directly produced from the initial substrate (GlcNAc)6 but produced through transglycosylation reaction
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
glycol chitin + H2O
?
show the reaction diagram
glycol chitin + H2O
chitobiose + chitotriose + chitotetraose
show the reaction diagram
-
-
-
-
?
glycol chitin + H2O
chitotriose
show the reaction diagram
glycol chitin + H2O
N,N'-diacetylchitobiose + GlcNAc
show the reaction diagram
-
exochitinase activity that yields p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose, and then when the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is accumulated, the p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose is further cleaved into N,N'-diacetylchitobiose and GlcNAc
-
-
?
glycol chitin + H2O
N-acetylglucosamine
show the reaction diagram
glycol chitodextrin + H2O
?
show the reaction diagram
-
slow
-
-
?
glycol chitosan + H2O
?
show the reaction diagram
glycolchitin + H2O
?
show the reaction diagram
hexa-N-acetyl-chitohexaose + H2O
?
show the reaction diagram
hexa-N-acetyl-chitohexaose + H2O
di-N-acetyl-chitobiose + tri-N-acetyl-chitotriose
show the reaction diagram
lactic acid-treated crab shell + H2O
?
show the reaction diagram
methylumbelliferyl-beta-N',N',N'-triacetyl-chitotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
methylumbelliferyl-beta-N',N'-diacetyl-chitobioside + H2O
?
show the reaction diagram
-
-
-
-
?
methylumbelli