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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of a novel type of chitinase from Vibrio alginolyticus TK-22 (1992), Biosci. Biotechnol. Biochem., 56, 368-369.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Vibrio alginolyticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
gel filtration | Vibrio alginolyticus |
| 66000 | - |
SDS-PAGE | Vibrio alginolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio alginolyticus | - |
- |
- |
| Vibrio alginolyticus TK-22 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 22.5fold to homogeneity | Vibrio alginolyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Vibrio alginolyticus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | colloidal chitin | Vibrio alginolyticus | N,N-diacetylchitobiose + N-acetylglucosamine | also chitotriose, chitotetraose, chitopentaose | ? |  |
| chitin + H2O | colloidal chitin | Vibrio alginolyticus TK-22 | N,N-diacetylchitobiose + N-acetylglucosamine | also chitotriose, chitotetraose, chitopentaose | ? | |
| glycol chitin + H2O | - |
Vibrio alginolyticus | N-acetylglucosamine + ? | - |
? | |
| glycol chitin + H2O | - |
Vibrio alginolyticus TK-22 | N-acetylglucosamine + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Vibrio alginolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable below 40°C for 30 min | Vibrio alginolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
first optimum | Vibrio alginolyticus |
| 9 | - |
second optimum | Vibrio alginolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | stable for 20 hours | Vibrio alginolyticus |
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