Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Lee, Y.G.; Chung, K.C.; Wi, S.G.; Lee, J.C.; Bae, H.J.
    Purification and properties of a chitinase from Penicillium sp. LYG 0704 (2009), Protein Expr. Purif., 65, 244-250.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 79% inhibition at 1 mM Penicillium sp.
Hg2+ 81% inhibition at 1 mM Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 26% activation at 1 mM Penicillium sp.
Mo2+ 22% activation at 1 mM Penicillium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, SDS-PAGE Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Penicillium sp. degradation pattern of chitin, overview ?
-
 ?
chitin + H2O Penicillium sp. LYG 0704 degradation pattern of chitin, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
-
-
Penicillium sp. LYG 0704
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular chitinase 17.6fold by isopropanol precipitation, anion exchange chromatography, and hydrophobic interaction chromatography Penicillium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.3
-
 purified extracellular chitinase Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Penicillium sp. ?
-
 ?
chitin + H2O degradation pattern of chitin, overview Penicillium sp. ?
-
 ?
chitin + H2O
-
 Penicillium sp. LYG 0704 ?
-
 ?
chitin + H2O degradation pattern of chitin, overview Penicillium sp. LYG 0704 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Penicillium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 temperature profile, overview Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Penicillium sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 8 pH profile, overview Penicillium sp.