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Literature summary for 3.2.1.14 extracted from
- Production and characterization of a thermostable chitinase from a new alkalophilic Bacillus sp. BG-11 (2000), J. Appl. Microbiol., 88, 800-808.
General Stability
| General Stability | Organism |
|---|---|
| survives 10 freezings and thawings without loss of activity. Retains 90% and 80% of its activity in presence of trypsin and rennilase respectively | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chitobiose | - |
Bacillus sp. (in: Bacteria) |  |
| chitotriose | - |
Bacillus sp. (in: Bacteria) | |
| D-galactosamine | - |
Bacillus sp. (in: Bacteria) | |
| D-glucosamine | - |
Bacillus sp. (in: Bacteria) | |
| N-acetylglucosamine | - |
Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 54.29 | - |
chitin | in N-acetylglucasmine equivalents | Bacillus sp. (in: Bacteria) | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
BG-11 | - |
| Bacillus sp. (in: Bacteria) BG-11 | - |
BG-11 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 16fold | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 8 weeks: no loss of activity, 10 weeks: 80% of activity remains in presence of NaN3, sodium metabisulfite or NaCl, 25°C, 4 weeks: no loss of activity in presence of NaN3, sodium metabisulfite or NaCl and glycerol, 8 weeks: 75% of activity remains in presence of NaN3, sodium metabisulfite or NaCl and glycerol | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | swollen chitin | Bacillus sp. (in: Bacteria) | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylglucosamine only | ? | |
| chitin + H2O | swollen chitin | Bacillus sp. (in: Bacteria) BG-11 | N,N-diacetylchitobiose + N-acetylglucosamine | N-acetylglucosamine only | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | immobilized chitinase | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half life 90 min at 70°C, immobilized chitinase | Bacillus sp. (in: Bacteria) |
| 80 | - |
half life 70 min at 80°C, immobilized chitinase | Bacillus sp. (in: Bacteria) |
| 90 | - |
half life 60 min at 90°C, immobilized chitinase | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | immobilized chitinase | Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | immobilized chitinase | Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | immobilized chitinase | Bacillus sp. (in: Bacteria) |
| 6 | 9 | free enzyme | Bacillus sp. (in: Bacteria) |
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