KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Annona cherimola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14309 | - |
1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry | Annona cherimola |
14310 | - |
1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry | Annona cherimola |
14500 | - |
native PAGE | Annona cherimola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Annona cherimola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 36.2fold from the mesocarp of fruits by ultrafiltration, ammonium sulfate fractionation, anion exchange chromatography and chromatofocusing | Annona cherimola |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | mesocarp of CO2-treated cherimoya fruit | Annona cherimola | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3458 | - |
purified enzyme, pH 5.0, 37°C | Annona cherimola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O | - |
Annona cherimola | ? | - |
? | |
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O | - |
Annona cherimola | ? | - |
? | |
4-methylumbelliferyl chitobioside + H2O | - |
Annona cherimola | 4-methylumbelliferone + chitobiose | - |
? | |
CM-chitin-RBV + H2O | i.e carboxymethyl-Chitin-Remazol-Brilliant-Violet, a colloidal substrate | Annona cherimola | ? | - |
? | |
additional information | no activity with 4-methylumbelliferyl N-acetylglucosamine | Annona cherimola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14310, SDS-PAGE, 1 * 14309, mass spectrometry | Annona cherimola |
More | peptide mapping by MALDI-TOF mass spectroemtry | Annona cherimola |
Synonyms | Comment | Organism |
---|---|---|
BChi14 | - |
Annona cherimola |
endochitinase | - |
Annona cherimola |
More | the enzyme belongs to the family 19 of glycosyl hydrolases, GH19 | Annona cherimola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Annona cherimola |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 50 | 30% of maximal activity at 5°C, 50% at 50°C | Annona cherimola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
purified enzyme, 30% activity remaining | Annona cherimola |
50 | - |
purified enzyme, stable up to, rapid loss of activity above | Annona cherimola |
60 | - |
purified enzyme, loss of 70% activity | Annona cherimola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.87 | - |
4-methylumbelliferyl chitobioside | pH 5.0, 37°C | Annona cherimola | |
6.93 | - |
4-methylumbelliferyl chitotriose | pH 5.0, 37°C | Annona cherimola | |
366.7 | - |
CM-chitin-RBV | pH 5.0, 37°C | Annona cherimola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Annona cherimola |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the chitinase isolated from cherimoya mesocarp apparently displays moderate enzymatic activity over a broad pH range, in both acidic and alkaline conditions, and with a neutral optimal pH | Annona cherimola |
3 | 9.5 | 80% of maximal activity at pH 3.0 and pH 9.0, 50% at pH 9.5 | Annona cherimola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Annona cherimola | isoelectric focusing | - |
8.26 |
General Information | Comment | Organism |
---|---|---|
physiological function | BChi14 is functionally implicated in the mechanisms underlying chilling tolerance activated by high CO2 concentrations. The purified endochitinase shows very strong cryoprotective activity against freeze-thaw inactivation of lactate dehydrogenase, but the enzyme has no effect on the inhibition of Botrytis cinerea hyphal growth and no antifreeze activity | Annona cherimola |